| Regulation of osteogenesis and osteoclastogenesis by zoledronic acid loaded on biodegradable magnesium-strontium alloy | |
| Li, Mei1,2; Wan, Peng3; Wang, Weidan4,5; Yang, Ke4; Zhang, Yu2; Han, Yong1 | |
| Corresponding Author | Wan, Peng(wanpeng@dgut.edu.cn) ; Zhang, Yu(luck_2001@126.com) ; Han, Yong(yonghan@mail.xjtu.edu.cn) |
| 2019-01-30 | |
| Source Publication | SCIENTIFIC REPORTS
 |
| ISSN | 2045-2322 |
| Volume | 9Pages:12 |
| Abstract | Inhibiting osteoclasts and osteoclast precursors to reduce bone resorption is an important strategy to treat osteoclast-related diseases, such as peri-prosthetic osteolysis. In this study, our objective was to study the role of zoledronic acid (ZA), as a highly potent and nitrogen-containing bisphosphonate, in promoting osteogenesis and inhibiting osteoclastogenesis properties of magnesium (Mg)-based implants. ZA was chemically associated with calcium phosphate (CaP) deposited on magnesiumstrontium (Mg-Sr) alloy, which was confirmed by the morphological observation, phase composition and drug releasing via SEM, XRD spectrum and High Performance Liquid Chromatography (HPLC), respectively. The in vitro performances indicated that ZA-CaP bilayer coating Mg-Sr alloy could enhance the proliferation and the osteogenic differentiation as well as the mineralization of pre-osteoblasts, however, induce the apoptosis and inhibit the osteoclast differentiation. We further investigated the possible molecular mechanisms by using Quantitative real-time PCR (qRT-PCR) and Western Blotting, and the results showed that ZA-CaP bilayer coating Mg-Sr alloy could regulate the osteogenesis and osteoclastogenesis through the Estrogen Receptor alpha (ER alpha) and NF-kappa B signaling pathway. Moreover, ZA-CaP bilayer coating Mg-Sr alloy could regulate the cross talk of osteoblast-osteoclast and increase the ratio of OPG: RANKL in the co-culture system through OPG/RANKL/RANK signaling pathway, which promoting the balance of bone remodeling process. Therefore, these promising results suggest the potential clinical applications of ZA pretreated Mg-Sr alloys for bone defect repairs and periprosthetical osteolysis due to the excessive differentitation and maturation of osteoclasts. |
| Funding Organization | National Natural Science Foundation of China ; National Key Research and Development Program of China ; Natural Science Foundation of Guangdong Province, China |
| DOI | 10.1038/s41598-018-37091-8 |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | National Natural Science Foundation of China[51371137] ; National Natural Science Foundation of China[81501859] ; National Natural Science Foundation of China[51631007] ; National Natural Science Foundation of China[31500777] ; National Natural Science Foundation of China[81601884] ; National Key Research and Development Program of China[2017YFB0702604] ; National Key Research and Development Program of China[2016YFB0700803] ; Natural Science Foundation of Guangdong Province, China[2015A030312004] |
| WOS Research Area | Science & Technology - Other Topics |
| WOS Subject | Multidisciplinary Sciences |
| WOS ID | WOS:000457128700010 |
| Publisher | NATURE PUBLISHING GROUP |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.imr.ac.cn/handle/321006/131155 |
| Collection | 中国科学院金属研究所 |
| Corresponding Author | Wan, Peng; Zhang, Yu; Han, Yong |
| Affiliation | 1.Xi An Jiao Tong Univ, State Key Lab Mech Behav Mat, Xian 710049, Shaanxi, Peoples R China 2.Guangdong Gen Hosp, Guangdong Acad Med Sci, Dept Orthoped, Guangzhou 510080, Guangdong, Peoples R China 3.Dongguan Univ Technol, Sch Mech Engn, Dongguan 523808, Peoples R China 4.Chinese Acad Sci, Inst Met Res, Shenyang 110016, Liaoning, Peoples R China 5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| Recommended Citation GB/T 7714 | Li, Mei,Wan, Peng,Wang, Weidan,et al. Regulation of osteogenesis and osteoclastogenesis by zoledronic acid loaded on biodegradable magnesium-strontium alloy[J]. SCIENTIFIC REPORTS,2019,9:12. |
| APA | Li, Mei,Wan, Peng,Wang, Weidan,Yang, Ke,Zhang, Yu,&Han, Yong.(2019).Regulation of osteogenesis and osteoclastogenesis by zoledronic acid loaded on biodegradable magnesium-strontium alloy.SCIENTIFIC REPORTS,9,12. |
| MLA | Li, Mei,et al."Regulation of osteogenesis and osteoclastogenesis by zoledronic acid loaded on biodegradable magnesium-strontium alloy".SCIENTIFIC REPORTS 9(2019):12. |
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