Regulation of osteogenesis and osteoclastogenesis by zoledronic acid loaded on biodegradable magnesium-strontium alloy

doi:10.1038/s41598-018-37091-8

IMR OpenIR

	Regulation of osteogenesis and osteoclastogenesis by zoledronic acid loaded on biodegradable magnesium-strontium alloy
	Li, Mei 1,2; Wan, Peng 3; Wang, Weidan 4,5; Yang, Ke 4; Zhang, Yu 2; Han, Yong 1
通讯作者	Wan, Peng(wanpeng@dgut.edu.cn) ; Zhang, Yu(luck_2001@126.com) ; Han, Yong(yonghan@mail.xjtu.edu.cn)
	2019-01-30
发表期刊	SCIENTIFIC REPORTS
ISSN	2045-2322
卷号	9 页码:12
摘要	Inhibiting osteoclasts and osteoclast precursors to reduce bone resorption is an important strategy to treat osteoclast-related diseases, such as peri-prosthetic osteolysis. In this study, our objective was to study the role of zoledronic acid (ZA), as a highly potent and nitrogen-containing bisphosphonate, in promoting osteogenesis and inhibiting osteoclastogenesis properties of magnesium (Mg)-based implants. ZA was chemically associated with calcium phosphate (CaP) deposited on magnesiumstrontium (Mg-Sr) alloy, which was confirmed by the morphological observation, phase composition and drug releasing via SEM, XRD spectrum and High Performance Liquid Chromatography (HPLC), respectively. The in vitro performances indicated that ZA-CaP bilayer coating Mg-Sr alloy could enhance the proliferation and the osteogenic differentiation as well as the mineralization of pre-osteoblasts, however, induce the apoptosis and inhibit the osteoclast differentiation. We further investigated the possible molecular mechanisms by using Quantitative real-time PCR (qRT-PCR) and Western Blotting, and the results showed that ZA-CaP bilayer coating Mg-Sr alloy could regulate the osteogenesis and osteoclastogenesis through the Estrogen Receptor alpha (ER alpha) and NF-kappa B signaling pathway. Moreover, ZA-CaP bilayer coating Mg-Sr alloy could regulate the cross talk of osteoblast-osteoclast and increase the ratio of OPG: RANKL in the co-culture system through OPG/RANKL/RANK signaling pathway, which promoting the balance of bone remodeling process. Therefore, these promising results suggest the potential clinical applications of ZA pretreated Mg-Sr alloys for bone defect repairs and periprosthetical osteolysis due to the excessive differentitation and maturation of osteoclasts.
资助者	National Natural Science Foundation of China ; National Key Research and Development Program of China ; Natural Science Foundation of Guangdong Province, China
DOI	10.1038/s41598-018-37091-8
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[51371137] ; National Natural Science Foundation of China[81501859] ; National Natural Science Foundation of China[51631007] ; National Natural Science Foundation of China[31500777] ; National Natural Science Foundation of China[81601884] ; National Key Research and Development Program of China[2017YFB0702604] ; National Key Research and Development Program of China[2016YFB0700803] ; Natural Science Foundation of Guangdong Province, China[2015A030312004]
WOS研究方向	Science & Technology - Other Topics
WOS类目	Multidisciplinary Sciences
WOS记录号	WOS:000457128700010
出版者	NATURE PUBLISHING GROUP
引用统计	被引频次：62[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/131160
专题	中国科学院金属研究所
通讯作者	Wan, Peng; Zhang, Yu; Han, Yong
作者单位	1.Xi An Jiao Tong Univ, State Key Lab Mech Behav Mat, Xian 710049, Shaanxi, Peoples R China 2.Guangdong Gen Hosp, Guangdong Acad Med Sci, Dept Orthoped, Guangzhou 510080, Guangdong, Peoples R China 3.Dongguan Univ Technol, Sch Mech Engn, Dongguan 523808, Peoples R China 4.Chinese Acad Sci, Inst Met Res, Shenyang 110016, Liaoning, Peoples R China 5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Li, Mei,Wan, Peng,Wang, Weidan,et al. Regulation of osteogenesis and osteoclastogenesis by zoledronic acid loaded on biodegradable magnesium-strontium alloy[J]. SCIENTIFIC REPORTS,2019,9:12.
APA	Li, Mei,Wan, Peng,Wang, Weidan,Yang, Ke,Zhang, Yu,&Han, Yong.(2019).Regulation of osteogenesis and osteoclastogenesis by zoledronic acid loaded on biodegradable magnesium-strontium alloy.SCIENTIFIC REPORTS,9,12.
MLA	Li, Mei,et al."Regulation of osteogenesis and osteoclastogenesis by zoledronic acid loaded on biodegradable magnesium-strontium alloy".SCIENTIFIC REPORTS 9(2019):12.