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Osteogenesis stimulation by copper-containing 316L stainless steel via activation of akt cell signaling pathway and Runx2 upregulation
Yuan, Yonghui1,2; Jin, Shujing3; Qi, Xun1; Chen, Xudong4,5; Zhang, Wei4,5; Yang, Ke3; Zhong, Hongshan1
Corresponding AuthorYang, Ke(kyang@imr.ac.cn) ; Zhong, Hongshan(hszhong@cmu.edu.cn)
2019-11-01
Source PublicationJOURNAL OF MATERIALS SCIENCE & TECHNOLOGY
ISSN1005-0302
Volume35Issue:11Pages:2727-2733
AbstractAs a metallic orthopedic implant, 316L stainless steel (316L SS) is used extensively for its good resistance to corrosion and mechanical properties. However, it takes a long time to achieve osseointegration between 316 L SS and adjacent tissues due to its bio-inert characteristic. Hence, the aim is to improve the bio-adaption of 316 L SS. A good approach is to add elements to materials to improve their osteogenic capabilities by the appropriate release of ions. Hence copper-containing 316 L stainless steel (316L-Cu SS) was investigated in this work, where Cu is an essential trace element that can stimulates osteogenesis. It was found that 316L-Cu SS was bio-safe and did not affect the proliferation of co-cultured osteoblasts in comparison with 316 L SS. It increased cell apoptosis on day 1 but inhibited it on day 3, which cooperates with new bone formation processes. Osteoblasts extend themselves more quickly and in a better manner on the surface of 316L-Cu SS, wheneven more pseudopodia are present. Furthermore, the gene expression of alkaline phosphatase, collagen I and runt-related transcription factor 2 (Runx2) in osteoblasts cultured with 316L-Cu SS was significantly enhanced. Runx2 protein expression increased, and osteogenesis was stimulated by 316L-Cu SS via an Akt cell signaling pathway. In conclusion, 316L-Cu SS stimulates osteogenesis through activation of the Akt cell signaling pathway and the upregulation of Runx2. Thus, 316L-Cu SS is a promising material that may be used in surgical implants to stimulate osteogenesis. (C) 2019 Published by Elsevier Ltd on behalf of The editorial office of Journal of Materials Science & Technology.
KeywordOsteogenesis Cu addition Stainless steel Cell signaling pathway Bone
Funding OrganizationNational Key Research and Development Program of China ; National Natural Science Foundation ; Science and Technology Plan of Shenyang
DOI10.1016/j.jmst.2019.04.028
Indexed BySCI
Language英语
Funding ProjectNational Key Research and Development Program of China[2018YFC1106600] ; National Natural Science Foundation[81571778] ; National Natural Science Foundation[51631009] ; Science and Technology Plan of Shenyang[17-230-9-42] ; Science and Technology Plan of Shenyang[18-014-4-28]
WOS Research AreaMaterials Science ; Metallurgy & Metallurgical Engineering
WOS SubjectMaterials Science, Multidisciplinary ; Metallurgy & Metallurgical Engineering
WOS IDWOS:000497965900034
PublisherJOURNAL MATER SCI TECHNOL
Citation statistics
Cited Times:6[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.imr.ac.cn/handle/321006/136203
Collection中国科学院金属研究所
Corresponding AuthorYang, Ke; Zhong, Hongshan
Affiliation1.China Med Univ, Affiliated Hosp 1, Dept Radiol, Key Lab Diagnost Imaging & Intervent Radiol Liaon, Shenyang 110001, Liaoning, Peoples R China
2.China Med Univ, Liaoning Canc Hosp & Inst, Canc Hosp, Res & Acad Dept, Shenyang 110042, Liaoning, Peoples R China
3.Chinese Acad Sci, Inst Met Res, Shenyang 110016, Liaoning, Peoples R China
4.Southern Univ Sci & Technol, Affiliated Hosp 1, Shenzhen Peoples Hosp, Shenzhen 518020, Guangdong, Peoples R China
5.Shenzhen Intervent Med Engn Res & Dev Ctr, Shenzhen 518020, Guangdong, Peoples R China
Recommended Citation
GB/T 7714
Yuan, Yonghui,Jin, Shujing,Qi, Xun,et al. Osteogenesis stimulation by copper-containing 316L stainless steel via activation of akt cell signaling pathway and Runx2 upregulation[J]. JOURNAL OF MATERIALS SCIENCE & TECHNOLOGY,2019,35(11):2727-2733.
APA Yuan, Yonghui.,Jin, Shujing.,Qi, Xun.,Chen, Xudong.,Zhang, Wei.,...&Zhong, Hongshan.(2019).Osteogenesis stimulation by copper-containing 316L stainless steel via activation of akt cell signaling pathway and Runx2 upregulation.JOURNAL OF MATERIALS SCIENCE & TECHNOLOGY,35(11),2727-2733.
MLA Yuan, Yonghui,et al."Osteogenesis stimulation by copper-containing 316L stainless steel via activation of akt cell signaling pathway and Runx2 upregulation".JOURNAL OF MATERIALS SCIENCE & TECHNOLOGY 35.11(2019):2727-2733.
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