| Improved osteogenic differentiation of human amniotic mesenchymal stem cells on gradient nanostructured Ti surface | |
| Wang, Wei1; Wang, Zhenbo2; Fu, Yating1; Dunne, Nicholas3; Liang, Chen2; Luo, Xue1; Liu, Keda1; Li, Xiaoming4,5; Pang, Xining1,6; Lu, Ke2 | |
| Corresponding Author | Wang, Zhenbo(zbwang@imr.ac.cn) ; Li, Xiaoming(x.m.li@hotmail.com) ; Pang, Xining(pangxining@126.com) |
| 2020-09-01 | |
| Source Publication | JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
 |
| ISSN | 1549-3296 |
| Volume | 108Issue:9Pages:1824-1833 |
| Abstract | Titanium (Ti) and Ti-based alloys are widely used in the manufacture of dental and orthopedic implants. However, how to improve their osteogenic differentiation ability is still a key issue to be resolved. In this study, gradient nanostructured surface (GNS) samples were prepared by surface mechanical grinding treatment, and coarse-grained (CG) samples were obtained by recrystallization annealing, making sure that the two kinds of specimens had similar roughness. Then, human amniotic mesenchymal stem cells (hAMSCs) were cocultured with the two kinds of Ti to investigate the material effects on the cellular functions. The results demonstrated that the grains with size similar to 56 nm were formed on the surface of the GNS Ti, and the grain size gradually increases from the sample surface to interior. Compared to the CG samples, the GNS ones could make the adhesion effect of the hAMSCs better, and promote the cell proliferation and osteogenic differentiation more significantly, the preliminary mechanism of which might be due to their specific nanostructure, the thicker oxide layer formed on their surface and the enhanced hardness. Our results indicated that the gradient nanostructured Ti materials could enhance both osteogenic differentiation and mechanical properties, which may possess broader applications in bone tissue engineering and clinical implanting. |
| Keyword | gradient nanostructured human amniotic mesenchymal stem cells osteogenic differentiation surface mechanical attrition treatment titanium (Ti) |
| Funding Organization | Liaoning Provincial Natural Science Foundation Guidance Project ; Central Government of Liaoning Province to Guide Local Science and Technology Development Project ; International Joint Research Center of Aerospace Biotechnology and Medical Engineering, Ministry of Science and Technology of China ; 111 Project ; Liaoning Province, Colleges and Universities Basic Research Project ; Liaoning Provincial Key Research Plan Guidance Project ; National Natural Science Foundation of China ; Shenyang Major Scientific and Technological Innovation Research and Development Plan ; Second Batch of Medical Education Scientific Research Projects of the 13th Five-Year Plan of China Medical University |
| DOI | 10.1002/jbm.a.36948 |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | Liaoning Provincial Natural Science Foundation Guidance Project[2019-ZD-0749] ; Central Government of Liaoning Province to Guide Local Science and Technology Development Project[2017108001] ; International Joint Research Center of Aerospace Biotechnology and Medical Engineering, Ministry of Science and Technology of China[B13003] ; 111 Project[B13003] ; Liaoning Province, Colleges and Universities Basic Research Project[LFWK201717] ; Liaoning Provincial Key Research Plan Guidance Project[2018225078] ; National Natural Science Foundation of China[31771042] ; National Natural Science Foundation of China[81970980] ; Shenyang Major Scientific and Technological Innovation Research and Development Plan[19-112-4-027] ; Second Batch of Medical Education Scientific Research Projects of the 13th Five-Year Plan of China Medical University[YDJK2018017] |
| WOS Research Area | Engineering ; Materials Science |
| WOS Subject | Engineering, Biomedical ; Materials Science, Biomaterials |
| WOS ID | WOS:000541582000001 |
| Publisher | WILEY |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.imr.ac.cn/handle/321006/139532 |
| Collection | 中国科学院金属研究所 |
| Corresponding Author | Wang, Zhenbo; Li, Xiaoming; Pang, Xining |
| Affiliation | 1.China Med Univ, Sch & Hosp Stomatol, Liaoning Prov Key Lab Oral Dis, Shenyang 110001, Liaoning, Peoples R China 2.Chinese Acad Sci, Shenyang Natl Lab Mat Sci, Inst Met Res, 72 Wenhua Rd, Shenyang 110016, Liaoning, Peoples R China 3.Dublin City Univ, Sch Mech & Mfg Engn, Ctr Med Engn Res, Dublin, Ireland 4.Beihang Univ, Sch Biol Sci & Med Engn, Key Lab Biomech & Mechanobiol, Minist Educ, Beijing 100083, Peoples R China 5.Beihang Univ, Beijing Adv Innovat Ctr Biomed Engn, Beijing, Peoples R China 6.China Med Univ, Key Lab Cell Biol, Shenyang, Liaoning, Peoples R China |
| Recommended Citation GB/T 7714 | Wang, Wei,Wang, Zhenbo,Fu, Yating,et al. Improved osteogenic differentiation of human amniotic mesenchymal stem cells on gradient nanostructured Ti surface[J]. JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A,2020,108(9):1824-1833. |
| APA | Wang, Wei.,Wang, Zhenbo.,Fu, Yating.,Dunne, Nicholas.,Liang, Chen.,...&Lu, Ke.(2020).Improved osteogenic differentiation of human amniotic mesenchymal stem cells on gradient nanostructured Ti surface.JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A,108(9),1824-1833. |
| MLA | Wang, Wei,et al."Improved osteogenic differentiation of human amniotic mesenchymal stem cells on gradient nanostructured Ti surface".JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A 108.9(2020):1824-1833. |
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