Biofunctional magnesium coated Ti6Al4V scaffold enhances osteogenesis and angiogenesis in vitro and in vivo for orthopedic application<bold> </bold>

doi:10.1016/j.bioactmat.2020.04.019

IMR OpenIR

	Biofunctional magnesium coated Ti6Al4V scaffold enhances osteogenesis and angiogenesis in vitro and in vivo for orthopedic application
	Gao, Peng 1,2,3; Fan, Bo 1; Yu, Xiaoming 4,5; Liu, Wenwen 1; Wu, Jie 1; Shi, Lei 1; Yang, Di 1; Tan, Lili 4; Wan, Peng 4; Hao, Yulin 4; Li, Shujun 4; Hou, Wentao 4; Yang, Ke 4; Li, Xiaokang 1; Guo, Zheng 1
通讯作者	Yang, Ke(kyang@imr.ac.cn) ; Li, Xiaokang(lxkfmmu@163.com) ; Guo, Zheng(guozheng@fmmu.edu.cn)
	2020-09-01
发表期刊	BIOACTIVE MATERIALS
卷号	5 期号:3 页码:680-693
摘要	The insufficient osteogenesis and osseointegration of porous titanium based scaffold limit its further application. Early angiogenesis is important for scaffold survival. It is necessary to develop a multifunctional surface on titanium scaffold with both osteogenic and angiogenic properties. In this study, a biofunctional magnesium coating is deposited on porous Ti6Al4V scaffold. For osseointegration and osteogenesis analysis, in vitro studies reveal that magnesium-coated Ti6Al4V co-culture with MC3T3-E1 cells can improve cell proliferation, adhesion, extracellular matrix (ECM) mineralization and ALP activity compared with bare Ti6Al4V cocultivation. Additionally, MC3T3-E1 cells cultured with magnesium-coated Ti6Al4V show significantly higher osteogenesis-related genes expression. In vivo studies including fluorochrome labeling, micro-computerized tomography and histological examination of magnesium-coated Ti6Al4V scaffold reveal that new bone regeneration is significantly increased in rabbits after implantation. For angiogenesis studies, magnesium-coated Ti6Al4V improve HUVECs proliferation, adhesion, tube formation, wound-healing and Transwell abilities. HUVECs cultured with magnesium-coated Ti6Al4V display significantly higher angiogenesis-related genes (HIF-1 alpha and VEGF) expression. Microangiography analysis reveal that magnesium-coated Ti6Al4V scaffold can significantly enhance the blood vessel formation. This study enlarges the application scope of magnesium and provides an optional choice to the conventional porous Ti6Al4V scaffold with enhanced osteogenesis and angiogenesis for further orthopedic applications.
关键词	Porous Ti6Al4V scafflod Surface modification Magnesium coating Osteogenesis Angiogenesis
资助者	National Key Research and Development Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of Youth Fund
DOI	10.1016/j.bioactmat.2020.04.019
收录类别	SCI
语种	英语
资助项目	National Key Research and Development Program of China[2017YFC1104901] ; National Natural Science Foundation of China[51871239] ; National Natural Science Foundation of China[51771227] ; National Natural Science Foundation of Youth Fund[51501223]
WOS研究方向	Engineering ; Materials Science
WOS类目	Engineering, Biomedical ; Materials Science, Biomaterials
WOS记录号	WOS:000546576100023
出版者	KEAI PUBLISHING LTD
引用统计	被引频次：119[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/139590
专题	中国科学院金属研究所
通讯作者	Yang, Ke; Li, Xiaokang; Guo, Zheng
作者单位	1.Fourth Mil Med Univ, Xijing Hosp, Dept Orthoped, Xian 710032, Peoples R China 2.Hunan Normal Univ, Dept Joint Surg & Sports Med, Hunan Prov Peoples Hosp, Changsha 410016, Peoples R China 3.Hunan Normal Univ, Affiliated Hosp 1, Changsha 410016, Peoples R China 4.Chinese Acad Sci, Inst Met Res, Shenyang 110016, Peoples R China 5.Shenyang Ligong Univ, Sch Mat Sci & Engn, Shenyang 110159, Peoples R China
推荐引用方式 GB/T 7714	Gao, Peng,Fan, Bo,Yu, Xiaoming,et al. Biofunctional magnesium coated Ti6Al4V scaffold enhances osteogenesis and angiogenesis in vitro and in vivo for orthopedic application [J]. BIOACTIVE MATERIALS,2020,5(3):680-693.
APA	Gao, Peng.,Fan, Bo.,Yu, Xiaoming.,Liu, Wenwen.,Wu, Jie.,...&Guo, Zheng.(2020).Biofunctional magnesium coated Ti6Al4V scaffold enhances osteogenesis and angiogenesis in vitro and in vivo for orthopedic application .BIOACTIVE MATERIALS,5(3),680-693.
MLA	Gao, Peng,et al."Biofunctional magnesium coated Ti6Al4V scaffold enhances osteogenesis and angiogenesis in vitro and in vivo for orthopedic application ".BIOACTIVE MATERIALS 5.3(2020):680-693.