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Bioinformatics analysis and identification of circular RNAs promoting the osteogenic differentiation of human bone marrow mesenchymal stem cells on titanium treated by surface mechanical attrition
Zhu, Shanshan1; Zhu, Yuhe1; Wang, Zhenbo2; Liang, Chen2; Cao, Nanjue3; Yan, Ming1; Gao, Fei1; Liu, Jie4; Wang, Wei1
Corresponding AuthorLiu, Jie(lj6152003@163.com) ; Wang, Wei(wwang75@cmu.edu.cn)
2020-07-13
Source PublicationPEERJ
ISSN2167-8359
Volume8Pages:22
AbstractBackground: To analyze and identify the circular RNAs (circRNAs) involved in promoting the osteogenic differentiation of human bone mesenchymal stem cells (hBMSCs) on titanium by surface mechanical attrition treatment (SMAT). Methods: The experimental material was SMAT titanium and the control material was annealed titanium. Cell Counting Kits-8 (CCK-8) was used to detect the proliferation of hBMSCs, and alkaline phosphatase (ALP) activity and alizarin red staining were used to detect the osteogenic differentiation of hBMSCs on the sample surfaces. The bioinformatics prediction software miwalk3.0 was used to construct competing endogenous RNA (ceRNA) networks by predicting circRNAs with osteogenesis-related messenger RNAs (mRNAs) and microRNAs (miRNAs). The circRNAs located at the key positions in the networks were selected and analyzed by quantitative real-time PCR (QRT-PCR). Results: Compared with annealed titanium, SMAT titanium could promote the proliferation and osteogenic differentiation of hBMSCs. The total number of predicted circRNAs was 51. Among these, 30 circRNAs and 8 miRNAs constituted 6 ceRNA networks. Circ-LTBP2 was selected for verification. QRT-PCR results showed that the expression levels of hsa_circ_0032599, hsa_circ_0032600 and hsa_circ_0032601 were upregulated in the experimental group compared with those in the control group; the differential expression of hsa_circ_0032600 was the most obvious and statistically significant, with a fold change (FC) = 4.25 +/- 1.60, p-values (p) <0.05.
KeywordSurface mechanical attrition treatment Human bone marrow mesenchymal stem cells Biological information prediction Osteogenic differentiation circRNA
Funding OrganizationNational Natural Science Foundation of China ; Liaoning Province, Colleges and Universities Basic Research Project ; Liaoning Provincial Key Research Plan Guidance Project ; Liaoning Provincial Natural Science Foundation Guidance Project ; Shenyang Major Scientific and Technological Innovation Research and Development Plan ; Shenyang Young and Middle-aged Technological Innovation Talent Plan ; Shenyang National Laboratory for Materials Science ; Second Batch of Medical Education Scientific Research Projects of the 13th Five-Year Plan of China Medical University
DOI10.7717/peerj.9292
Indexed BySCI
Language英语
Funding ProjectNational Natural Science Foundation of China[81970980] ; Liaoning Province, Colleges and Universities Basic Research Project[LFWK201717] ; Liaoning Provincial Key Research Plan Guidance Project[2018225078] ; Liaoning Provincial Natural Science Foundation Guidance Project[2019-ZD-0749] ; Shenyang Major Scientific and Technological Innovation Research and Development Plan[19-112-4-027] ; Shenyang Young and Middle-aged Technological Innovation Talent Plan[RC200060] ; Shenyang National Laboratory for Materials Science[2015RP04] ; Second Batch of Medical Education Scientific Research Projects of the 13th Five-Year Plan of China Medical University[YDJK2018017]
WOS Research AreaScience & Technology - Other Topics
WOS SubjectMultidisciplinary Sciences
WOS IDWOS:000547882300001
PublisherPEERJ INC
Citation statistics
Cited Times:3[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.imr.ac.cn/handle/321006/139657
Collection中国科学院金属研究所
Corresponding AuthorLiu, Jie; Wang, Wei
Affiliation1.China Med Univ, Sch & Hosp Stomatol, Liaoning Prov Key Lab Oral Dis, Shenyang, Liaoning, Peoples R China
2.Chinese Acad Sci, Inst Met Res, Shenyang Natl Lab Mat Sci, Shenyang, Liaoning, Peoples R China
3.Zhejiang Univ, Affiliated Hosp 4, Sch Med, Yiwu, Zhejiang, Peoples R China
4.China Med Univ, Dept Sci Expt Ctr 1, Shenyang, Liaoning, Peoples R China
Recommended Citation
GB/T 7714
Zhu, Shanshan,Zhu, Yuhe,Wang, Zhenbo,et al. Bioinformatics analysis and identification of circular RNAs promoting the osteogenic differentiation of human bone marrow mesenchymal stem cells on titanium treated by surface mechanical attrition[J]. PEERJ,2020,8:22.
APA Zhu, Shanshan.,Zhu, Yuhe.,Wang, Zhenbo.,Liang, Chen.,Cao, Nanjue.,...&Wang, Wei.(2020).Bioinformatics analysis and identification of circular RNAs promoting the osteogenic differentiation of human bone marrow mesenchymal stem cells on titanium treated by surface mechanical attrition.PEERJ,8,22.
MLA Zhu, Shanshan,et al."Bioinformatics analysis and identification of circular RNAs promoting the osteogenic differentiation of human bone marrow mesenchymal stem cells on titanium treated by surface mechanical attrition".PEERJ 8(2020):22.
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