Construction of EGFP-tagged rBCG of E.tenella and distribution in chickens

IMR OpenIR

	Construction of EGFP-tagged rBCG of E.tenella and distribution in chickens
其他题名	Construction of EGFP-tagged rBCG of E.tenella and distribution in chickens
	Wang QiuYue 1; Li JianHua 1; Zhang XiChen 1; Liu ChengWu 2; Cao LiLi 1; Ren KeYan 3; Gong PengTao 1; Cai YaNan 1
	2009
发表期刊	SCIENCE IN CHINA SERIES C-LIFE SCIENCES
ISSN	1006-9305
卷号	52 期号:3 页码:278-283
摘要	Chicken coccidiosis is a major parasitic disease with substantial economic burden to the poultry industry. Enhanced green fluorescent protein (EGFP) tagged recombinant Bacille Calmette-Guerin (rBCG), as a fusion protein with coccidian rhomboid antigen was constructed to track rBCG in vivo in chickens in this study. Immunization of chickens with one dose of rBCG pMV361-Rho/EGFP induced humoral immune response. The colonization of rBCG in liver, spleen, lung, kidney and caecum was observed by laser confocal microscopy. Real-time quantitative RT-PCR showed a rise expression level of rhomboid protein on the 7th day and a peak on the 14th day and disappearance on the 28th day after immunization. These results have significant implications for the development of rBCG vaccines against avian coccidiosis.
其他摘要	Chicken coccidiosis is a major parasitic disease with substantial economic burden to the poultry in-dustry. Enhanced green fluorescent protein (EGFP) tagged recombinant Bacille Calmette-Guerin (rBCG), as a fusion protein with coccidian rhomboid antigen was constructed to track rBCG in vivo in chickens in this study. Immunization of chickens with one dose of rBCG pMV361-Rho/EGFP induced humoral immune response. The colonization of rBCG in liver, spleen, lung, kidney and caecum was observed by laser confocal microscopy. Real-time quantitative RT-PCR showed s rise expression level of rhomboid protein on the 7th day and a peak on the 14th day and disappearance on the 28th day after immunization. These results have significant implications for the development of rBCG vaccines against avian coccidiosis.
关键词	IMMUNE-RESPONSES ANTIGEN DELIVERY EIMERIA-TENELLA VACCINE VECTORS BCG COCCIDIOSIS PROTECTION CHALLENGE IMMUNIZATION TOXOPLASMA coccidiosis rBCG EGFP rhomboid laser confocal microscopy real-time RT-PCR
收录类别	CSCD
语种	英语
CSCD记录号	CSCD:3590941
引用统计
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/141857
专题	中国科学院金属研究所
作者单位	1.吉林大学 2.中国科学院金属研究所 3.中山大学
推荐引用方式 GB/T 7714	Wang QiuYue,Li JianHua,Zhang XiChen,et al. Construction of EGFP-tagged rBCG of E.tenella and distribution in chickens[J]. SCIENCE IN CHINA SERIES C-LIFE SCIENCES,2009,52(3):278-283.
APA	Wang QiuYue.,Li JianHua.,Zhang XiChen.,Liu ChengWu.,Cao LiLi.,...&Cai YaNan.(2009).Construction of EGFP-tagged rBCG of E.tenella and distribution in chickens.SCIENCE IN CHINA SERIES C-LIFE SCIENCES,52(3),278-283.
MLA	Wang QiuYue,et al."Construction of EGFP-tagged rBCG of E.tenella and distribution in chickens".SCIENCE IN CHINA SERIES C-LIFE SCIENCES 52.3(2009):278-283.