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Investigation of Interaction Mechanism Between Signaling Molecule Hydrogen Sulfide and L-Lactic Dehydrogenase
Alternative TitleInvestigation of Interaction Mechanism Between Signaling Molecule Hydrogen Sulfide and L-Lactic Dehydrogenase
Zhu YanWen1; Liu Ling1; Sun WeiJie1; Yan TingCai1; Tan DeHong1; Zhang Yao2; Bai Bing1
2018
Source PublicationCHINESE JOURNAL OF ANALYTICAL CHEMISTRY
ISSN0253-3820
Volume46Issue:7Pages:1145-1151
AbstractMolecular mechanisms whereby H2 S influences its targets have been of intriguing interest. In this work, L-lactic dehydrogenase (L-LDH) was used as the protein target, and three kinds of H2 S-donor reagents ( NaHS, Na2S, and polysulfide) were chosen. The interactions of these H2 S-donor reagents with L-LDH were disclosed by molecular fluorescent assays for real-time monitoring of L-LDH activity. The results of the SDSPAGE showed that H2 S might not interact with L-LDH to form disulfide/trisulfide bonding. Circular dichroism spectra assays revealed that H2 S reagents could be likely to react with cysteine thiols to yield sulfurated thiol ( SSH) derivatives in L-LDH, and sulfur-containing PS ( polysulfide) was a stronger protein S-sulfurating agent than the other two sulfides. Matrix assisted laser desorptionionization time-of-flight tandem mass spectrometry ( MALDI-TOF-MS/MS) study showed partial S-sulfuration of the active cysteine sites existed in L-LDH. In conclusion, H25 exerts its biological effects as a gasotransmitter through its reactions with cysteine thiols in proteins by S-sulfuration.
KeywordFRESH GARLIC SCALES H2S EXPLORATION OXIDATION Hydrogen sulfide L-Lactic dehydrogenase Cysteine thiols S-Sulfuration
Indexed ByCSCD
Language英语
Funding Project[National Natural Science Foundation of China] ; [National Key Research Projects of China]
CSCD IDCSCD:6284574
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Document Type期刊论文
Identifierhttp://ir.imr.ac.cn/handle/321006/142163
Collection中国科学院金属研究所
Affiliation1.中国科学院金属研究所
2.沈阳师范大学
Recommended Citation
GB/T 7714
Zhu YanWen,Liu Ling,Sun WeiJie,et al. Investigation of Interaction Mechanism Between Signaling Molecule Hydrogen Sulfide and L-Lactic Dehydrogenase[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2018,46(7):1145-1151.
APA Zhu YanWen.,Liu Ling.,Sun WeiJie.,Yan TingCai.,Tan DeHong.,...&Bai Bing.(2018).Investigation of Interaction Mechanism Between Signaling Molecule Hydrogen Sulfide and L-Lactic Dehydrogenase.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,46(7),1145-1151.
MLA Zhu YanWen,et al."Investigation of Interaction Mechanism Between Signaling Molecule Hydrogen Sulfide and L-Lactic Dehydrogenase".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 46.7(2018):1145-1151.
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