IMR OpenIR
Variation of Potential Nitrification and Ammonia-Oxidizing Bacterial Community with Plant-Growing Period in Apple Orchard Soil
Alternative TitleVariation of Potential Nitrification and Ammonia-Oxidizing Bacterial Community with Plant-Growing Period in Apple Orchard Soil
Liu Lingzhi; Qin Sijun; Lu Deguo; Wang Bingying; Yang Zeyuan
2014
Source PublicationJOURNAL OF INTEGRATIVE AGRICULTURE
ISSN2095-3119
Volume13Issue:2Pages:415-425
AbstractIn this study, we investigated the potential nitrification and community structure of soil-based ammonia-oxidizing bacteria (AOB) in apple orchard soil during different growth periods and explored the effects of environmental factors on nitrification activity and AOB community composition in the soil of a Hanfu apple orchard, using a culture-dependent technique and denaturing gradient gel electrophoresis (DGGE). We observed that nitrification activity and AOB abundance were the highest in November, lower in May, and the lowest in July. The results of statistical analysis indicated that total nitrogen (N) content, NH4+-N content, NO3--N content, and pH showed significant correlations with AOB abundance and nitrification activity in soil, The Shannon-Winner diversity, as well as species richness and evenness indices (determined by PCR-DGGE banding patterns) in soil samples were the highest in September, but the lowest in July, when compared to additional sampled dates. The DGGE fingerprints of soil-based 16S rRNA genes in November were apparently distinct from those observed in May, July, and September, possessing the lowest species richness indices and the highest dominance indices among all four growth periods. Fourteen DGGE bands were excised for sequencing. The resulting analysis indicated that all AOB communities belonged to the beta-Proteobacteria phylum, with the dominant AOB showing high similarity to the Nitrosospira genus, Therefore, soil-based environmental factors, such as pH variation and content of NH4+-N and NO3--N, can substantially influence the abundance of AOB communities in soil, and play a critical role in soil-based nitrification kinetics.
Other AbstractIn this study, we investigated the potential nitriifcation and community structure of soil-based ammonia-oxidizing bacteria (AOB) in apple orchard soil during different growth periods and explored the effects of environmental factors on nitriifcation activity and AOB community composition in the soil of a Hanfu apple orchard, using a culture-dependent technique and denaturing gradient gel electrophoresis (DGGE). We observed that nitrification activity and AOB abundance were the highest in November, lower in May, and the lowest in July. The results of statistical analysis indicated that total nitrogen (N) content, NH4+-N content, NO3--N content, and pH showed signiifcant correlations with AOB abundance and nitriifcation activity in soil. The Shannon-Winner diversity, as well as species richness and evenness indices (determined by PCR-DGGE banding patterns) in soil samples were the highest in September, but the lowest in July, when compared to additional sampled dates. The DGGE ifngerprints of soil-based 16S rRNA genes in November were apparently distinct from those observed in May, July, and September, possessing the lowest species richness indices and the highest dominance indices among all four growth periods. Fourteen DGGE bands were excised for sequencing. The resulting analysis indicated that all AOB communities belonged to the β-Proteobacteriaphylum, with the dominant AOB showing high similarity to theNitrosospira genus. Therefore, soil-based environmental factors, such as pH variation and content of NH4+-N and NO3--N, can substantially inlfuence the abundance of AOB communities in soil, and play a critical role in soil-based nitriifcation kinetics.
KeywordGRADIENT GEL-ELECTROPHORESIS CONIFEROUS FOREST SOILS 16S RIBOSOMAL-RNA NITRIFYING BACTERIA LOW PH PCR DIVERSITY ACID POPULATIONS ARCHAEA apple orchard soil ammonia-oxidizing bacteria potential nitrification community structure PCR-denaturing gradient gel electrophoresis
Indexed ByCSCD
Language英语
Funding Project[National Natural Science Foundation of China] ; [Postdoctoral Science Foundation of China] ; [China Agricultural Research System] ; [Shenyang Municipal Science and Technology Research Projects, China]
CSCD IDCSCD:5048474
Citation statistics
Document Type期刊论文
Identifierhttp://ir.imr.ac.cn/handle/321006/142394
Collection中国科学院金属研究所
Affiliation中国科学院金属研究所
Recommended Citation
GB/T 7714
Liu Lingzhi,Qin Sijun,Lu Deguo,et al. Variation of Potential Nitrification and Ammonia-Oxidizing Bacterial Community with Plant-Growing Period in Apple Orchard Soil[J]. JOURNAL OF INTEGRATIVE AGRICULTURE,2014,13(2):415-425.
APA Liu Lingzhi,Qin Sijun,Lu Deguo,Wang Bingying,&Yang Zeyuan.(2014).Variation of Potential Nitrification and Ammonia-Oxidizing Bacterial Community with Plant-Growing Period in Apple Orchard Soil.JOURNAL OF INTEGRATIVE AGRICULTURE,13(2),415-425.
MLA Liu Lingzhi,et al."Variation of Potential Nitrification and Ammonia-Oxidizing Bacterial Community with Plant-Growing Period in Apple Orchard Soil".JOURNAL OF INTEGRATIVE AGRICULTURE 13.2(2014):415-425.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Liu Lingzhi]'s Articles
[Qin Sijun]'s Articles
[Lu Deguo]'s Articles
Baidu academic
Similar articles in Baidu academic
[Liu Lingzhi]'s Articles
[Qin Sijun]'s Articles
[Lu Deguo]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Liu Lingzhi]'s Articles
[Qin Sijun]'s Articles
[Lu Deguo]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.