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Determination of Total Arsenic and Arsenic Metabolites in Human Liver Hepatocytes
Alternative TitleDetermination of Total Arsenic and Arsenic Metabolites in Human Liver Hepatocytes
Cao Xuan1; Yu JingJing2; Wang Geng3; Yu ZhenHua2; Yang HuangHao2; Xu YuanYuan5; Sun GuiFan5; Wang XiaoRu2
2009
Source PublicationCHINESE JOURNAL OF ANALYTICAL CHEMISTRY
ISSN0253-3820
Volume37Issue:1Pages:19-24
AbstractThe effects of direct sampling and three digestion methods were investigated on the determination of arsenic in Chang liver hepatocytes after ultrasonic disintegration were investigated. The results showed that the efficiency of microwave digestion and obturator digestion was better than cold digestion and direct sampling. The day precision (present as RSD) of microwave digestion and obturator digestion were 2.1% and 1.2% the inter-day precision were 1.2% and 2.0%, respectively. The spike recovery for the total As in the sample is 95.7% - 108.1%. The As detection limits with these four sample treatment methods (including direct sampling) were 0.74 - 0.93 mu g/L. In addition, arsenic speciation in Chang liver hepatocytes was also analyzed using the hyphenated technique of high performance liquid chromatography coupled with inductively coupled plasma-mass spectrometry. The experimental results indicated that dimethylarsinic acid (DMA) and an intermediate metabolite of DMA were found lit Chang liver hepatocytes besides inorganic arsenic (As(III) and As(V)).
Other AbstractThe effects of direct sampling and three digestion methods were investigated on the determination of arsenic in chang liver hepatocytes after ultrasonic disintegration were investigated. the results showed that the efficiency of microwave digestion and obturator digestion was better than cold digestion and direct sampling. the day precision (present as rsd) of microwave digestion and obturator digestion were 2.1% and 1.2% the inter-day precision were 1.2% and 2.0%, respectively. the spike recovery for the total as in the sample is 95.7% - 108.1%. the as detection limits with these four sample treatment methods (including direct sampling) were 0.74 - 0.93 mu g/l. in addition, arsenic speciation in chang liver hepatocytes was also analyzed using the hyphenated technique of high performance liquid chromatography coupled with inductively coupled plasma-mass spectrometry. the experimental results indicated that dimethylarsinic acid (dma) and an intermediate metabolite of dma were found lit chang liver hepatocytes besides inorganic arsenic (as(iii) and as(v)).
KeywordINGESTION TOXICITY RATS ACID Cell digestion high performance liquid chromatography-inductively coupled plasma-mass sepetrometry Chang liver hepatocytes arsenic speciation
Indexed ByCSCD
Language英语
CSCD IDCSCD:3486776
Citation statistics
Document Type期刊论文
Identifierhttp://ir.imr.ac.cn/handle/321006/142679
Collection中国科学院金属研究所
Affiliation1.陕西省地震局
2.黑龙江省环境保护科学研究院
3.山东大学
4.中国科学院
5.中国科学院金属研究所
Recommended Citation
GB/T 7714
Cao Xuan,Yu JingJing,Wang Geng,et al. Determination of Total Arsenic and Arsenic Metabolites in Human Liver Hepatocytes[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2009,37(1):19-24.
APA Cao Xuan.,Yu JingJing.,Wang Geng.,Yu ZhenHua.,Yang HuangHao.,...&Wang XiaoRu.(2009).Determination of Total Arsenic and Arsenic Metabolites in Human Liver Hepatocytes.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,37(1),19-24.
MLA Cao Xuan,et al."Determination of Total Arsenic and Arsenic Metabolites in Human Liver Hepatocytes".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 37.1(2009):19-24.
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