Determination of Total Arsenic and Arsenic Metabolites in Human Liver Hepatocytes

IMR OpenIR

	Determination of Total Arsenic and Arsenic Metabolites in Human Liver Hepatocytes
其他题名	Determination of Total Arsenic and Arsenic Metabolites in Human Liver Hepatocytes
	Cao Xuan 1; Yu JingJing 2; Wang Geng 3; Yu ZhenHua 2; Yang HuangHao 2; Xu YuanYuan 5; Sun GuiFan 5; Wang XiaoRu 2
	2009
发表期刊	CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
ISSN	0253-3820
卷号	37 期号:1 页码:19-24
摘要	The effects of direct sampling and three digestion methods were investigated on the determination of arsenic in Chang liver hepatocytes after ultrasonic disintegration were investigated. The results showed that the efficiency of microwave digestion and obturator digestion was better than cold digestion and direct sampling. The day precision (present as RSD) of microwave digestion and obturator digestion were 2.1% and 1.2% the inter-day precision were 1.2% and 2.0%, respectively. The spike recovery for the total As in the sample is 95.7% - 108.1%. The As detection limits with these four sample treatment methods (including direct sampling) were 0.74 - 0.93 mu g/L. In addition, arsenic speciation in Chang liver hepatocytes was also analyzed using the hyphenated technique of high performance liquid chromatography coupled with inductively coupled plasma-mass spectrometry. The experimental results indicated that dimethylarsinic acid (DMA) and an intermediate metabolite of DMA were found lit Chang liver hepatocytes besides inorganic arsenic (As(III) and As(V)).
其他摘要	The effects of direct sampling and three digestion methods were investigated on the determination of arsenic in chang liver hepatocytes after ultrasonic disintegration were investigated. the results showed that the efficiency of microwave digestion and obturator digestion was better than cold digestion and direct sampling. the day precision (present as rsd) of microwave digestion and obturator digestion were 2.1% and 1.2% the inter-day precision were 1.2% and 2.0%, respectively. the spike recovery for the total as in the sample is 95.7% - 108.1%. the as detection limits with these four sample treatment methods (including direct sampling) were 0.74 - 0.93 mu g/l. in addition, arsenic speciation in chang liver hepatocytes was also analyzed using the hyphenated technique of high performance liquid chromatography coupled with inductively coupled plasma-mass spectrometry. the experimental results indicated that dimethylarsinic acid (dma) and an intermediate metabolite of dma were found lit chang liver hepatocytes besides inorganic arsenic (as(iii) and as(v)).
关键词	INGESTION TOXICITY RATS ACID Cell digestion high performance liquid chromatography-inductively coupled plasma-mass sepetrometry Chang liver hepatocytes arsenic speciation
收录类别	CSCD
语种	英语
CSCD记录号	CSCD:3486776
引用统计	被引频次：6[CSCD] [CSCD记录]
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/142679
专题	中国科学院金属研究所
作者单位	1.陕西省地震局 2.黑龙江省环境保护科学研究院 3.山东大学 4.中国科学院 5.中国科学院金属研究所
推荐引用方式 GB/T 7714	Cao Xuan,Yu JingJing,Wang Geng,et al. Determination of Total Arsenic and Arsenic Metabolites in Human Liver Hepatocytes[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2009,37(1):19-24.
APA	Cao Xuan.,Yu JingJing.,Wang Geng.,Yu ZhenHua.,Yang HuangHao.,...&Wang XiaoRu.(2009).Determination of Total Arsenic and Arsenic Metabolites in Human Liver Hepatocytes.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,37(1),19-24.
MLA	Cao Xuan,et al."Determination of Total Arsenic and Arsenic Metabolites in Human Liver Hepatocytes".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 37.1(2009):19-24.