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Functional characterization of an invertase inhibitor gene involved in sucrose metabolism in tomato fruit
Alternative TitleFunctional characterization of an invertase inhibitor gene involved in sucrose metabolism in tomato fruit *
Zhang Ning; Jiang Jing; Yang Yanli; Wang Zhihe
2015
Source PublicationJOURNAL OF ZHEJIANG UNIVERSITY-SCIENCE B
ISSN1673-1581
Volume16Issue:10Pages:845-856
AbstractIn this study, we produced tomato plants overexpressing an invertase inhibitor gene (Sly-INH) from tomato, using a simple and efficient transient transformation system. Compared with control plants, the expression of Sly-INH was highly upregulated in Sly-INH overexpressing plants, as indicated by real-time polymerase chain reaction (PCR). Physiological analysis revealed that Sly-INH inhibited the activity of cell wall invertase (CWIN), which increased sugar accumulation in tomato fruit. Furthermore, Sly-INH mediated sucrose metabolism by regulating CWIN activity. Our results suggest that invertase activity is potentially regulated by the Sly-INH inhibitor at the post-translational level, and they demonstrate that the transient transformation system is an effective method for determining the functions of genes in tomato.
Other AbstractIn this study, we produced tomato plants overexpressing an invertase inhibitor gene ( Sly-INH ) from tomato, using a simple and efficient transient transformation system. Compared with control plants, the expression of Sly-INH was highly upregulated in Sly-INH overexpressing plants, as indicated by real-time polymerase chain reaction (PCR). Physiological analysis revealed that Sly-INH inhibited the activity of cell wall invertase (CWIN), which increased sugar accumulation in tomato fruit. Furthermore, Sly-INH mediated sucrose metabolism by regulating CWIN activity. Our results suggest that invertase activity is potentially regulated by the Sly-INH inhibitor at the post-translational level, and they demonstrate that the transient transformation system is an effective method for determining the functions of genes in tomato.
KeywordCELL-WALL INVERTASE APOPLASTIC INVERTASE VACUOLAR INVERTASE PLANT DEVELOPMENT CARBOHYDRATE-METABOLISM EXTRACELLULAR INVERTASE SOLANUM-LYCOPERSICON STRUCTURAL INSIGHTS ECTOPIC EXPRESSION CARBON METABOLISM Invertase inhibitor Fruit development Transient transformation system Solanum lycopersicum Overexpression
Indexed ByCSCD
Language英语
Funding Project[National Natural Science Foundation of China] ; [State Key Laboratory of Plant Physiology and Biochemistry Open Project of China]
CSCD IDCSCD:5544667
Citation statistics
Cited Times:2[CSCD]   [CSCD Record]
Document Type期刊论文
Identifierhttp://ir.imr.ac.cn/handle/321006/142722
Collection中国科学院金属研究所
Affiliation中国科学院金属研究所
Recommended Citation
GB/T 7714
Zhang Ning,Jiang Jing,Yang Yanli,et al. Functional characterization of an invertase inhibitor gene involved in sucrose metabolism in tomato fruit[J]. JOURNAL OF ZHEJIANG UNIVERSITY-SCIENCE B,2015,16(10):845-856.
APA Zhang Ning,Jiang Jing,Yang Yanli,&Wang Zhihe.(2015).Functional characterization of an invertase inhibitor gene involved in sucrose metabolism in tomato fruit.JOURNAL OF ZHEJIANG UNIVERSITY-SCIENCE B,16(10),845-856.
MLA Zhang Ning,et al."Functional characterization of an invertase inhibitor gene involved in sucrose metabolism in tomato fruit".JOURNAL OF ZHEJIANG UNIVERSITY-SCIENCE B 16.10(2015):845-856.
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