Functional characterization of an invertase inhibitor gene involved in sucrose metabolism in tomato fruit

IMR OpenIR

	Functional characterization of an invertase inhibitor gene involved in sucrose metabolism in tomato fruit
其他题名	Functional characterization of an invertase inhibitor gene involved in sucrose metabolism in tomato fruit *
	Zhang Ning; Jiang Jing; Yang Yanli; Wang Zhihe
	2015
发表期刊	JOURNAL OF ZHEJIANG UNIVERSITY-SCIENCE B
ISSN	1673-1581
卷号	16 期号:10 页码:845-856
摘要	In this study, we produced tomato plants overexpressing an invertase inhibitor gene (Sly-INH) from tomato, using a simple and efficient transient transformation system. Compared with control plants, the expression of Sly-INH was highly upregulated in Sly-INH overexpressing plants, as indicated by real-time polymerase chain reaction (PCR). Physiological analysis revealed that Sly-INH inhibited the activity of cell wall invertase (CWIN), which increased sugar accumulation in tomato fruit. Furthermore, Sly-INH mediated sucrose metabolism by regulating CWIN activity. Our results suggest that invertase activity is potentially regulated by the Sly-INH inhibitor at the post-translational level, and they demonstrate that the transient transformation system is an effective method for determining the functions of genes in tomato.
其他摘要	In this study, we produced tomato plants overexpressing an invertase inhibitor gene ( Sly-INH ) from tomato, using a simple and efficient transient transformation system. Compared with control plants, the expression of Sly-INH was highly upregulated in Sly-INH overexpressing plants, as indicated by real-time polymerase chain reaction (PCR). Physiological analysis revealed that Sly-INH inhibited the activity of cell wall invertase (CWIN), which increased sugar accumulation in tomato fruit. Furthermore, Sly-INH mediated sucrose metabolism by regulating CWIN activity. Our results suggest that invertase activity is potentially regulated by the Sly-INH inhibitor at the post-translational level, and they demonstrate that the transient transformation system is an effective method for determining the functions of genes in tomato.
关键词	CELL-WALL INVERTASE APOPLASTIC INVERTASE VACUOLAR INVERTASE PLANT DEVELOPMENT CARBOHYDRATE-METABOLISM EXTRACELLULAR INVERTASE SOLANUM-LYCOPERSICON STRUCTURAL INSIGHTS ECTOPIC EXPRESSION CARBON METABOLISM Invertase inhibitor Fruit development Transient transformation system Solanum lycopersicum Overexpression
收录类别	CSCD
语种	英语
资助项目	[National Natural Science Foundation of China] ; [State Key Laboratory of Plant Physiology and Biochemistry Open Project of China]
CSCD记录号	CSCD:5544667
引用统计	被引频次：4[CSCD] [CSCD记录]
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/142722
专题	中国科学院金属研究所
作者单位	中国科学院金属研究所
推荐引用方式 GB/T 7714	Zhang Ning,Jiang Jing,Yang Yanli,et al. Functional characterization of an invertase inhibitor gene involved in sucrose metabolism in tomato fruit[J]. JOURNAL OF ZHEJIANG UNIVERSITY-SCIENCE B,2015,16(10):845-856.
APA	Zhang Ning,Jiang Jing,Yang Yanli,&Wang Zhihe.(2015).Functional characterization of an invertase inhibitor gene involved in sucrose metabolism in tomato fruit.JOURNAL OF ZHEJIANG UNIVERSITY-SCIENCE B,16(10),845-856.
MLA	Zhang Ning,et al."Functional characterization of an invertase inhibitor gene involved in sucrose metabolism in tomato fruit".JOURNAL OF ZHEJIANG UNIVERSITY-SCIENCE B 16.10(2015):845-856.