Penicillium and patulin distribution in pears contaminated with Penicillium expansum. Determination of patulin in pears by UHPLCMS/MS

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	Penicillium and patulin distribution in pears contaminated with Penicillium expansum. Determination of patulin in pears by UHPLCMS/MS
其他题名	Penicillium and patulin distribution in pears contaminated with Penicillium expansum. Determination of patulin in pears by UHPLC-MS/MS
	Wei Dongmei 1; Xu Jun 1; Dong Fengshou 1; Liu Xingang 1; Wu Xiaohu 1; Zheng Yongquan 1
	2017
发表期刊	JOURNAL OF INTEGRATIVE AGRICULTURE
ISSN	2095-3119
卷号	16 期号:7 页码:1645-1651
摘要	The danger of mycotoxin contamination entering the food supply through post -harvest infection is of perennial concern to food safety experts. To explore the distribution of Penicillium expansum and diffusion of its mycotoxin, patulin, in blue mold -damaged pears, Pyrus bretschneideri Rehd. cv. Yali obtained from markets and orchards in China were artificially inoculated with P. expansum and assayed for patulin accumulation and degree of fungal colonization. The inoculated pears were incubated until the lesions were 5, 10, 20, or 30 mm in diameter. We sampled tissue at a range of distances from the lesion, measured the spread of Penicillium by plate colony-counting methods, and used UHPLC-MS/MS to detect and quantify the patulin concentration. More P. expansum colony-forming units were isolated from pears with a higher degree of decay. Farther from the lesion, the fewer P. expansum colonies were observed, and the lower the patulin content detected. We found a significant difference in the patulin content between samples due to lesion size, and also in tissue sampled 10 mm away from the lesion. In consideration of this finding, to ensure food safety, we recommend that when a blue mold rot lesion on pear is 5, 10, or 20 mm in diameter, 20, 30, and 40 mm beyond the lesion should be removed, respectively. If a lesion surpasses 30 mm in diameter, the whole pear should be thrown away.
其他摘要	The danger of mycotoxin contamination entering the food supply through post-harvest infection is of perennial concern to food safety experts. To explore the distribution of Penicillium expansum and diffusion of its mycotoxin, patulin, in blue mold-damaged pears, Pyrus bretschneideri Rehd. cv. Yali obtained from markets and orchards in China were artificially inoculated with P. expansum and assayed for patulin accumulation and degree of fungal colonization. The inoculated pears were incubated until the lesions were 5, 10, 20, or 30 mm in diameter. We sampled tissue at a range of distances from the lesion, measured the spread of Penicillium by plate colony-counting methods, and used UHPLC-MS/MS to detect and quantify the patulin concentration. More P. expansum colony-forming units were isolated from pears with a higher degree of decay. Farther from the lesion, the fewer P. expansum colonies were observed, and the lower the patulin content detected. We found a significant difference in the patulin content between samples due to lesion size, and also in tissue sampled 10 mm away from the lesion. In consideration of this finding, to ensure food safety, we recommend that when a blue mold rot lesion on pear is 5, 10, or 20 mm in diameter, 20, 30, and 40 mm beyond the lesion should be removed, respectively. If a lesion surpasses 30 mm in diameter, the whole pear should be thrown away.
关键词	MASS-SPECTROMETRY APPLE JUICE PRODUCTS DIFFUSION FRUITS FUNGI MODEL pear Penicillium expansum patulin food safety UHPLC-MS/MS
收录类别	CSCD
语种	英语
资助项目	[Quality and Safety Risk Assessment for Agro-products of China]
CSCD记录号	CSCD:6034706
引用统计
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/143644
专题	中国科学院金属研究所
作者单位	1.中国科学院亚热带农业生态研究所 2.中国科学院金属研究所
推荐引用方式 GB/T 7714	Wei Dongmei,Xu Jun,Dong Fengshou,et al. Penicillium and patulin distribution in pears contaminated with Penicillium expansum. Determination of patulin in pears by UHPLCMS/MS[J]. JOURNAL OF INTEGRATIVE AGRICULTURE,2017,16(7):1645-1651.
APA	Wei Dongmei,Xu Jun,Dong Fengshou,Liu Xingang,Wu Xiaohu,&Zheng Yongquan.(2017).Penicillium and patulin distribution in pears contaminated with Penicillium expansum. Determination of patulin in pears by UHPLCMS/MS.JOURNAL OF INTEGRATIVE AGRICULTURE,16(7),1645-1651.
MLA	Wei Dongmei,et al."Penicillium and patulin distribution in pears contaminated with Penicillium expansum. Determination of patulin in pears by UHPLCMS/MS".JOURNAL OF INTEGRATIVE AGRICULTURE 16.7(2017):1645-1651.