IMR OpenIR
Immunological rejection of acellular heterogeneous nerve transplant for bridging the sciatic nerve in rats
Alternative TitleImmunological rejection of acellular heterogeneous nerve transplant for bridging the sciatic nerve in rats
Jing Zhitao1; Zhang Haiying2; Zhang Xu1; Tong Xiaojie1
2010
Source PublicationNEURAL REGENERATION RESEARCH
ISSN1673-5374
Volume5Issue:9Pages:673-677
AbstractBACKGROUND: Artificial materials composed of acellular heterogeneous nerves can resolve donor shortage problems for the repair of peripheral nerve defects However, it remains unclear whether artificial materials can overcome immunological rejection of heterogeneous nerve grafts and obtain similar effects as allogeneic nerve grafts
Other AbstractBACKGROUND: Artificial materials composed of acellular heterogeneous nerves can resolve donor shortage problems for the repair of peripheral nerve defects. However, it remains unclear whether artificial materials can overcome immunological rejection of heterogeneous nerve grafts and obtain similar effects as allogeneic nerve grafts. OBJECTIVE: To analyze regeneration and immunological rejection of defective sciatic nerves in rats through the use of acellular heterogeneous nerve grafts. DESIGN, TIME AND SETTING: A randomized, controlled study was performed at the Department of Anatomy, China Medical University and the Experimental Center, First Affiliated Hospital, China Medical University between January and December 2008. MATERIALS: TritonX-100 (Sigma, USA) and deoxycholate (Pierce, USA) were used. METHODS: Bilateral sciatic nerves were collected from adult rabbits and treated with TritonX-100 and sodium deoxycholate to prepare acellular sciatic nerves, which were used to bridge 1-cm defective sciatic nerves in adult rats. MAIN OUTCOME MEASURES: The lymphocyte percentage in leukocytes was quantified following hemocyte staining. Neural regeneration and the recovery of motor end plates in the gastrocnemius muscle were observed under optical and electronic microscopy following toluidine blue staining, as well as acetylcholinesterase and succinate dehydrogenase histochemical staining. RESULTS: There was no significant difference in the lymphocyte percentage in leucocytes between transplanted and normal rats (P < 0.05). At 3 months after surgery, the rat toes on the operated side were separated and the rats could walk. In addition, the footplates exhibited an escape response when acupunctured. A large number of regenerated nerve fibers were observed in the transplant group, and acetylcholinesterase-positive motor end plates were visible in fibers of the gastrocnemius muscle. CONCLUSION: Acellular heterogeneous nerve transplants for the repair of defective sciatic nerves in rats promote neural regeneration without significant immunological rejection.
KeywordSCHWANN-CELLS CHEMICAL-EXTRACTION REGENERATION SCAFFOLDS GAP RECONSTRUCTION ALLOGRAFT RECOVERY heterogeneous nerve transplant peripheral nerve defect immunological rejection sciatic nerve neural regeneration
Indexed ByCSCD
Language英语
Funding Project[Science and Technology Research Foundation of Liaoning Provincial Department of Education]
CSCD IDCSCD:3893811
Citation statistics
Cited Times:3[CSCD]   [CSCD Record]
Document Type期刊论文
Identifierhttp://ir.imr.ac.cn/handle/321006/143772
Collection中国科学院金属研究所
Affiliation1.中国科学院金属研究所
2.辽宁大学
Recommended Citation
GB/T 7714
Jing Zhitao,Zhang Haiying,Zhang Xu,et al. Immunological rejection of acellular heterogeneous nerve transplant for bridging the sciatic nerve in rats[J]. NEURAL REGENERATION RESEARCH,2010,5(9):673-677.
APA Jing Zhitao,Zhang Haiying,Zhang Xu,&Tong Xiaojie.(2010).Immunological rejection of acellular heterogeneous nerve transplant for bridging the sciatic nerve in rats.NEURAL REGENERATION RESEARCH,5(9),673-677.
MLA Jing Zhitao,et al."Immunological rejection of acellular heterogeneous nerve transplant for bridging the sciatic nerve in rats".NEURAL REGENERATION RESEARCH 5.9(2010):673-677.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Jing Zhitao]'s Articles
[Zhang Haiying]'s Articles
[Zhang Xu]'s Articles
Baidu academic
Similar articles in Baidu academic
[Jing Zhitao]'s Articles
[Zhang Haiying]'s Articles
[Zhang Xu]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Jing Zhitao]'s Articles
[Zhang Haiying]'s Articles
[Zhang Xu]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.