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In vitro susceptibility testing of Aspergillus spp. against voriconazole, itraconazole, posaconazole, amphotericin B and caspofungin
Alternative TitleIn vitro susceptibility testing of Aspergillus spp. against voriconazole, itraconazole, posaconazole, amphotericin B and caspofungin
Shi Junyan1; Xu Yingchun1; Shi Yi2; Lue Huoxiang3; Liu Yong4; Zhao Wangsheng2; Chen Dongmei5; Xi Liyan5; Zhou Xin6; Wang He1; Guo Lina1
2010
Source PublicationCHINESE MEDICAL JOURNAL
ISSN0366-6999
Volume123Issue:19Pages:2706-2709
AbstractBackground During recent years, the incidence of serious infections caused by opportunistic fungi has increased dramatically due to alterations of the immune status of patients with hematological diseases, malignant tumors, transplantations and so forth. Unfortunately, the wide use of triazole antifungal agents to treat these infections has lead to the emergence of Aspergillus spp. resistant to triazoles. The present study was to assess the in vitro activities of five antifungal agents (voriconazole, itraconazole, posaconazole, amphotericin B and caspofungin) against different kinds of Aspergillus spp. that are commonly encountered in the clinical setting.
Other AbstractBackground During recent years, the incidence of serious infections caused by opportunistic fungi has increased dramatically due to alterations of the immune status of patients with hematological diseases, malignant tumors, transplantations and so forth. Unfortunately, the wide use of triazole antifungal agents to treat these infections has lead to the emergence of Aspergillus spp. resistant to triazoles. The present study was to assess the in vitro activities of five antifungal agents (voriconazole, itraconazole, posaconazole, amphotericin B and caspofungin) against different kinds of Aspergillus spp. that are commonly encountered in the clinical setting. Methods The agar-based Etest MIC method was employed. One hundred and seven strains of Aspergillus spp. (5 species) were collected and prepared according to Etest Technique Manuel. Etest MICs were determined with RPMI agar containing 2% glucose and were read after incubation for 48 hours at 35℃. MIC_(50), MIC_(90) and MIC range were acquired by Whonet 5.4 software. Results The MIC_(9o) of caspofungin against A. fumigatus, A. flavus and A. nidulans was 0.094 μg/ml whereas the MIC_(90) against A. niger was 0.19 μg/ml. For these four species, the MIC_(90) of caspofungin was the lowest among the five antifungal agents. For A. terrus, the MIC_(90) of posaconazole was the lowest. For A. fumigatus and A. flavus, the MIC_(90) in order of increasing was caspofungin, posaconazole, voriconazole, itraconazole, and amphotericin B. The MIC of amphotericin B against A. terrus was higher than 32 μg/ml in all 7 strains tested. Conclusions The in vitro antifungal susceptibility test shows the new drug caspofungin, which is a kind of echinocandins, has good activity against the five species of Aspergillus spp. and all the triazoles tested have better in vitro activity than traditional amphotericin B
KeywordMICRODILUTION METHODS FILAMENTOUS FUNGI ETEST FUMIGATUS COMBINATION EFFICACY MODEL MICS aspergillus antifungal agents drug resistance antifungal activity
Indexed ByCSCD
Language英语
Funding Project[Ministry of Health for public profession]
CSCD IDCSCD:4033721
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Document Type期刊论文
Identifierhttp://ir.imr.ac.cn/handle/321006/145123
Collection中国科学院金属研究所
Affiliation1.中国科学院
2.中国科学院南京地理与湖泊研究所
3.Zhejiang Prov Peoples Hosp, Clin Lab, Hangzhou 310014, Zhejiang, Peoples R China
4.中国科学院金属研究所
5.中国科学院广州地球化学研究所
6.Shanghai First Peoples Hosp, Department Resp, Shanghai 200080, Peoples R China
Recommended Citation
GB/T 7714
Shi Junyan,Xu Yingchun,Shi Yi,et al. In vitro susceptibility testing of Aspergillus spp. against voriconazole, itraconazole, posaconazole, amphotericin B and caspofungin[J]. CHINESE MEDICAL JOURNAL,2010,123(19):2706-2709.
APA Shi Junyan.,Xu Yingchun.,Shi Yi.,Lue Huoxiang.,Liu Yong.,...&Guo Lina.(2010).In vitro susceptibility testing of Aspergillus spp. against voriconazole, itraconazole, posaconazole, amphotericin B and caspofungin.CHINESE MEDICAL JOURNAL,123(19),2706-2709.
MLA Shi Junyan,et al."In vitro susceptibility testing of Aspergillus spp. against voriconazole, itraconazole, posaconazole, amphotericin B and caspofungin".CHINESE MEDICAL JOURNAL 123.19(2010):2706-2709.
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