In vitro susceptibility testing of Aspergillus spp. against voriconazole, itraconazole, posaconazole, amphotericin B and caspofungin

IMR OpenIR

	In vitro susceptibility testing of Aspergillus spp. against voriconazole, itraconazole, posaconazole, amphotericin B and caspofungin
其他题名	In vitro susceptibility testing of Aspergillus spp. against voriconazole, itraconazole, posaconazole, amphotericin B and caspofungin
	Shi Junyan 1; Xu Yingchun 1; Shi Yi 2; Lue Huoxiang 3; Liu Yong 4; Zhao Wangsheng 2; Chen Dongmei 5; Xi Liyan 5; Zhou Xin 6; Wang He 1; Guo Lina 1
	2010
发表期刊	CHINESE MEDICAL JOURNAL
ISSN	0366-6999
卷号	123 期号:19 页码:2706-2709
摘要	Background During recent years, the incidence of serious infections caused by opportunistic fungi has increased dramatically due to alterations of the immune status of patients with hematological diseases, malignant tumors, transplantations and so forth. Unfortunately, the wide use of triazole antifungal agents to treat these infections has lead to the emergence of Aspergillus spp. resistant to triazoles. The present study was to assess the in vitro activities of five antifungal agents (voriconazole, itraconazole, posaconazole, amphotericin B and caspofungin) against different kinds of Aspergillus spp. that are commonly encountered in the clinical setting.
其他摘要	Background During recent years, the incidence of serious infections caused by opportunistic fungi has increased dramatically due to alterations of the immune status of patients with hematological diseases, malignant tumors, transplantations and so forth. Unfortunately, the wide use of triazole antifungal agents to treat these infections has lead to the emergence of Aspergillus spp. resistant to triazoles. The present study was to assess the in vitro activities of five antifungal agents (voriconazole, itraconazole, posaconazole, amphotericin B and caspofungin) against different kinds of Aspergillus spp. that are commonly encountered in the clinical setting. Methods The agar-based Etest MIC method was employed. One hundred and seven strains of Aspergillus spp. (5 species) were collected and prepared according to Etest Technique Manuel. Etest MICs were determined with RPMI agar containing 2% glucose and were read after incubation for 48 hours at 35℃. MIC_(50), MIC_(90) and MIC range were acquired by Whonet 5.4 software. Results The MIC_(9o) of caspofungin against A. fumigatus, A. flavus and A. nidulans was 0.094 μg/ml whereas the MIC_(90) against A. niger was 0.19 μg/ml. For these four species, the MIC_(90) of caspofungin was the lowest among the five antifungal agents. For A. terrus, the MIC_(90) of posaconazole was the lowest. For A. fumigatus and A. flavus, the MIC_(90) in order of increasing was caspofungin, posaconazole, voriconazole, itraconazole, and amphotericin B. The MIC of amphotericin B against A. terrus was higher than 32 μg/ml in all 7 strains tested. Conclusions The in vitro antifungal susceptibility test shows the new drug caspofungin, which is a kind of echinocandins, has good activity against the five species of Aspergillus spp. and all the triazoles tested have better in vitro activity than traditional amphotericin B
关键词	MICRODILUTION METHODS FILAMENTOUS FUNGI ETEST FUMIGATUS COMBINATION EFFICACY MODEL MICS aspergillus antifungal agents drug resistance antifungal activity
收录类别	CSCD
语种	英语
资助项目	[Ministry of Health for public profession]
CSCD记录号	CSCD:4033721
引用统计
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/145123
专题	中国科学院金属研究所
作者单位	1.中国科学院 2.中国科学院南京地理与湖泊研究所 3.Zhejiang Prov Peoples Hosp, Clin Lab, Hangzhou 310014, Zhejiang, Peoples R China 4.中国科学院金属研究所 5.中国科学院广州地球化学研究所 6.Shanghai First Peoples Hosp, Department Resp, Shanghai 200080, Peoples R China
推荐引用方式 GB/T 7714	Shi Junyan,Xu Yingchun,Shi Yi,et al. In vitro susceptibility testing of Aspergillus spp. against voriconazole, itraconazole, posaconazole, amphotericin B and caspofungin[J]. CHINESE MEDICAL JOURNAL,2010,123(19):2706-2709.
APA	Shi Junyan.,Xu Yingchun.,Shi Yi.,Lue Huoxiang.,Liu Yong.,...&Guo Lina.(2010).In vitro susceptibility testing of Aspergillus spp. against voriconazole, itraconazole, posaconazole, amphotericin B and caspofungin.CHINESE MEDICAL JOURNAL,123(19),2706-2709.
MLA	Shi Junyan,et al."In vitro susceptibility testing of Aspergillus spp. against voriconazole, itraconazole, posaconazole, amphotericin B and caspofungin".CHINESE MEDICAL JOURNAL 123.19(2010):2706-2709.