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Expression of recombinant tryptophan decarboxylase in different subcellular compartments in tobacco plant
Alternative TitleExpression of recombinant tryptophan decarboxylase in different subcellular compartments in tobacco plant
Wang M1; Li QR1; Di Fiore S1; Fischer R1
2002
Source PublicationACTA BOTANICA SINICA
ISSN0577-7496
Volume44Issue:3Pages:314-317
AbstractThe gene encoded for tryptophan decarboxylase (TDC), which is the key enzyme in terpenoil indole alkaloids pathway, was targeted to different subcellular compartments and stably expressed in transgenic tobacco (Nicotiana tabacum L.) plants at the levels detected by Western blot and tryptamine accumulation analysis. It was shown that the TDC was located in subcellular compartments, the chloroplasts and cytosol. The recombinant TDC targeted to chloroplasts and cytosol in tobacco plants was effectively expressed as soluble protein by Western blot analysis and enzymatic assay. The level of tryptamine accumulation in chloroplast was higher than that in cytosol and very low in vacuole and endoplasmic reticulum (ER) to be hardly detected by Western blot analysis. It was indicated that the highest amount of tryptamine was in chloroplasts, lower in endoplasmic reticula and the lowest in vacuoles as compared to those in wild type plants. The TDC targeted to different subcellular compartments of tobacco plants and its expression level were studied by different nucleotide sequences coding signal peptides at 5'-end of tdc gene in order to know the effects of the TDC in compartmentation on its functionality.
Other AbstractThe gene encoded for tryptophan decarboxylase (TDC), which is the key enzyme in terpenoil indole alkaloids pathway, was targeted to different subcellular compartments and stably expressed in transgenic tobacco (Nicotiana tabacum L.) plants at the levels detected by Western blot and tryptamine accumulation analysis. It was shown that the TDC was located in subcellular compartments, the chloroplasts and cytosol. The recombinant TDC targeted to chloroplasts and cytosol in tobacco plants was effectively expressed as soluble protein by Western blot analysis and enzymatic assay. The level of tryptamine accumulation in chloroplast was higher than that in cytosol and very low in vacuole and endoplasmic reticulum (ER) to be hardly detected by Western blot analysis. It was indicated that the highest amount of tryptamine was in chloroplasts, lower in endoplasmic reticula and the lowest in vacuoles as compared to those in wild type plants. The TDC targeted to different subcellular compartments of tobacco plants and its expression level were studied by different nucleotide sequences coding signal peptides at 5'-end of tdc gene in order to know the effects of the TDC in compartmentation on its functionality.
KeywordCATHARANTHUS-ROSEUS ALKALOIDS PROTEINS ER tryptophan decarboxylase alkaloids biosynthetic pathway Nicotiana tabacum subcellular compartment
Indexed ByCSCD
Language英语
CSCD IDCSCD:1365022
Citation statistics
Document Type期刊论文
Identifierhttp://ir.imr.ac.cn/handle/321006/145597
Collection中国科学院金属研究所
Affiliation1.中国科学院
2.中国科学院金属研究所
3.Rhein Westfal TH Aachen
Recommended Citation
GB/T 7714
Wang M,Li QR,Di Fiore S,et al. Expression of recombinant tryptophan decarboxylase in different subcellular compartments in tobacco plant[J]. ACTA BOTANICA SINICA,2002,44(3):314-317.
APA Wang M,Li QR,Di Fiore S,&Fischer R.(2002).Expression of recombinant tryptophan decarboxylase in different subcellular compartments in tobacco plant.ACTA BOTANICA SINICA,44(3),314-317.
MLA Wang M,et al."Expression of recombinant tryptophan decarboxylase in different subcellular compartments in tobacco plant".ACTA BOTANICA SINICA 44.3(2002):314-317.
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