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LC-MS/MS Method for the Quantitation of Cefotetan in Human Plasma and Its Application to Pharmacokinetic Study
Alternative TitleLC-MS/MS Method for the Quantitation of Cefotetan in Human Plasma and Its Application to Pharmacokinetic Study
Shi Meiyun1; Yin Lei1; Cai Lanlan1; Wang Can1; Liu Xidong1; Zhao Sen1; Sun Yantong1; Fawcett J Paul3; Zhao Limei4; Yang Yan1; Gu Jingkai1
2014
Source PublicationCHEMICAL RESEARCH IN CHINESE UNIVERSITIES
ISSN1005-9040
Volume30Issue:6Pages:900-904
AbstractA rapid and sensitive liquid chromatography-tandem mass spectrometry(LC-MS/MS) method for the determination of cefotetan in human plasma was developed and validated. After the protein precipitation of sample with acetonitrile, the analyte and internal standard(IS), tramadol, were separated on a Zorbax XDB C-8 column using acetonitrile/1%(volume fraction) formic acid(volume ratio 35:65, pH=2.5) as mobile phase at a flow rate of 1.0 mL/min with a 1:1 split. The detection was performed by electrospray ionization with positive ion mode, followed by multiple reaction monitoring of the transitions for cefotetan at m/z 576.3 -> 460.2(quantifier) and m/z 576.3 -> 432.2(qualifier) and for IS at m/z 264.1 -> 58.1. Cefotetan and IS were eluted at 1.86 and 1.87 min, respectively. The assay was linear over the concentration range of 0.1 -> 100 mu g/mL for 20 mu L of human plasma only with intra-and inter-day precisions(expressed as the relative standard deviation) of less than 6.62% and accuracies(as relative error) of +/- 1.31%. The method was applied to the pharmacokinetic study of a 1-h intravenous infusion of 1.0 g of cefotetan disodium for human volunteers(n=6).
Other AbstractA rapid and sensitive liquid chromatography-tandem mass spectrometry(LC-MS/MS) method for the determination of cefotetan in human plasma was developed and validated. After the protein precipitation of sample with acetonitrile, the analyte and internal standard(IS), tramadol, were separated on a Zorbax XDB C_8 column using acetonitrile/1%(volume fraction) formic acid(volume ratio 35:65, pH=2.5) as mobile phase at a flow rate of 1.0 mL/min with a 1:1 split. The detection was performed by electrospray ionization with positive ion mode, followed by multiple reaction monitoring of the transitions for cefotetan at m/z 576.3→460.2(quantifier) and m/z 576.3→432.2(qualifier) and for IS at m/z 264.1→58.1. Cefotetan and IS were eluted at 1.86 and 1.87 min, respectively. The assay was linear over the concentration range of 0.1―100 μg/mL for 20 μL of human plasma only with intra- and inter-day precisions(expressed as the relative standard deviation) of less than 6.62% and accuracies(as relative error) of ±1.31%. The method was applied to the pharmacokinetic study of a 1-h intravenous infusion of 1.0 g of cefotetan disodium for human volunteers(n=6).
KeywordHEALTHY CHINESE VOLUNTEERS DISODIUM SINGLE INJECTION Cefotetan Liquid chromatography-tandem mass spectrometry(LC-MS/MS) Pharmacokinetics Human plasma
Indexed ByCSCD
Language英语
Funding Project[National Natural Science Foundation of China] ; [Preject of China Equipment and Education Resources System(CERS)]
CSCD IDCSCD:5301737
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Document Type期刊论文
Identifierhttp://ir.imr.ac.cn/handle/321006/145676
Collection中国科学院金属研究所
Affiliation1.吉林大学
2.中山大学
3.University Otago, Sch Pharm, Dunedin 9054, New Zealand
4.中国科学院金属研究所
Recommended Citation
GB/T 7714
Shi Meiyun,Yin Lei,Cai Lanlan,et al. LC-MS/MS Method for the Quantitation of Cefotetan in Human Plasma and Its Application to Pharmacokinetic Study[J]. CHEMICAL RESEARCH IN CHINESE UNIVERSITIES,2014,30(6):900-904.
APA Shi Meiyun.,Yin Lei.,Cai Lanlan.,Wang Can.,Liu Xidong.,...&Gu Jingkai.(2014).LC-MS/MS Method for the Quantitation of Cefotetan in Human Plasma and Its Application to Pharmacokinetic Study.CHEMICAL RESEARCH IN CHINESE UNIVERSITIES,30(6),900-904.
MLA Shi Meiyun,et al."LC-MS/MS Method for the Quantitation of Cefotetan in Human Plasma and Its Application to Pharmacokinetic Study".CHEMICAL RESEARCH IN CHINESE UNIVERSITIES 30.6(2014):900-904.
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