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镁合金表面沉积β-磷酸三钙涂层的单细胞凝胶电泳实验
Alternative TitleSingle cell gel electrophoresis of a magnesium alloy coated with β-tricalcium phosphate
郝玉全1; 谭丽丽2; 颜廷亭2; 阎秀林1; 杨柯2; 艾红军1
2009
Source Publication中华口腔医学杂志
ISSN1002-0098
Volume44.0Issue:010Pages:626-628
Abstract目的研究表面沉积β-磷酸三钙涂层的镁合金对人外周淋巴细胞DNA的影响,以期为涂层镁合金的临床应用提供依据。方法采用化学沉积法在镁合金表面沉积β-磷酸三钙涂层(镁合金涂层组),同时设镁合金组、阴性对照组(纯钛)和阳性对照组(质量浓度为0.5mg/L的博来霉素);进行单细胞凝胶电泳实验,每组观察60个细胞,计算4组尾矩和尾部DNA百分含量,进行单因素方差分析和SNK检验。结果镁合金组尾矩为(0.52±0.12),尾部DNA百分含量为(6.82±1.81)%;镁合金涂层组尾矩为(0.51±0.12),尾部DNA百分含量为(6.89±1.93)%;镁合金组与镁合金涂层组相比,差异均无统计学意义(P〉0.05)。阴性对照组尾矩为(0.47±0.14),尾部DNA百分含量为(6.29±1.64)%;阳性对照组尾矩为(5.17±1.23),尾部DNA百分含量为(22.09±4.51)%。结论沉积涂层后的镁合金对人外周血淋巴细胞DNA的损伤程度未增加。
Other AbstractObjective To evaluate the genotoxicity of a magnesium alloy coated with β-tricalcium phosphate(β-TCP). Methods Four groups were designed. In the first group, AZ31B magnesium alloy surface was coated with β-TCP using chemical bath deposition, and in the second group magnesium alloy was tested. The other two groups were negative control (pure titanium ) and positive control groups (0.5mg/L bleomycin). Single cell gel electrophoresis was adopted to investigate genotoxicity of the alloy samples in different groups, and 60 cells from each group were analysed. Tail moment and tail DNA percentage were used as reliable indicators to show DNA damage in lymphocytes induced by every testing sample. Student- Newman-Keuls(SNK) test was used to compare results from 4 groups. Results There were no significant differences in tail moment and tail DNA percentage between magnesium alloy group (0.52±0.12), (6.82±1.81)% and magnesium alloy coated with β-TCP group(0.51±0.12), (6.89±1.93)% ,P 〉0.05. Tail moment and tail DNA percentage in negative group were (0.47±0.14) and (6.29±1.64)%, and tail moment and tail DNA percentage in positive group were (5.17±1.23) and (22.09±4.51)%. Conclusions No significant increase was found in DNA damage in lymphocytes induced by magnesium alloy coated with β-TCP.
Keyword 磷酸钙类 彗星试验
Indexed ByCSCD
Language中文
CSCD IDCSCD:3674382
Citation statistics
Document Type期刊论文
Identifierhttp://ir.imr.ac.cn/handle/321006/145914
Collection中国科学院金属研究所
Affiliation1.中国医科大学
2.中国科学院金属研究所
Recommended Citation
GB/T 7714
郝玉全,谭丽丽,颜廷亭,等. 镁合金表面沉积β-磷酸三钙涂层的单细胞凝胶电泳实验[J]. 中华口腔医学杂志,2009,44.0(010):626-628.
APA 郝玉全,谭丽丽,颜廷亭,阎秀林,杨柯,&艾红军.(2009).镁合金表面沉积β-磷酸三钙涂层的单细胞凝胶电泳实验.中华口腔医学杂志,44.0(010),626-628.
MLA 郝玉全,et al."镁合金表面沉积β-磷酸三钙涂层的单细胞凝胶电泳实验".中华口腔医学杂志 44.0.010(2009):626-628.
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