| Stem cell properties and neural differentiation of sheep amniotic epithelial cells | |
| Alternative Title | Stem cell properties and neural differentiation of sheep amniotic epithelial cells ☆ |
| Zhu Xuemin1; Wang Xiumei1; Cao Guifang1; Liu Fengjun2; Yang Yinfeng1; Li Xiaonan3; Zhang Yuling2; Mi Yan1; Liu Junping1; Zhang Lingli1 | |
| 2013 | |
| Source Publication | NEURAL REGENERATION RESEARCH
 |
| ISSN | 1673-5374 |
| Volume | 8Issue:13Pages:1210-1219 |
| Abstract | This study was designed to verify the stem cell properties of sheep amniotic epithelial cells and their capacity for neural differentiation. Immunofluorescence microscopy and reverse transcription-PCR revealed that the sheep amniotic epithelial cells were positive for the embryonic stem cell marker proteins SSEA-1, SSEA-3, SSEA-4, TRA-1-60 and TRA-1-81, and the totipotency-associated genes Oct-4, Sox-2 and Rex-1, but negative for Nanog. Amniotic epithelial cells expressed beta-III-tubulin, glial fibrillary acidic protein, nestin and microtubule-associated protein-2 at 28 days after induction with serum-free neurobasal-A medium containing B-27. Thus, sheep amniotic epithelial cells could differentiate into neurons expressing beta-III-tubulin and microtubule-associated protein-2, and glial-like cells expressing glial fibrillary acidic protein, under specific conditions. |
| Other Abstract | This study was designed to verify the stem cell properties of sheep amniotic epithelial cells and their capacity for neural differentiation. Immunofluorescence microscopy and reverse transcription-PCR revealed that the sheep amniotic epithelial cells were positive for the embryonic stem cell marker proteins SSEA-1, SSEA-3, SSEA-4, TRA-1-60 and TRA-1-81, and the totipotency-associated genes Oct-4, Sox-2 and Rex-1, but negative for Nanog. Amniotic epithelial cells expressed β-III-tubulin, glial fibrillary acidic protein, nestin and microtubule-associated protein-2 at 28 days after induction with serum-free neurobasal-A medium containing B-27. Thus, sheep amniotic epithelial cells could differentiate into neurons expressing β-III-tubulin and microtubule-associated protein-2, and glial-like cells expressing glial fibrillary acidic protein, under specific conditions. |
| Keyword | PRIMORDIAL GERM-CELLS IN-VITRO PARKINSONS-DISEASE HUMAN BLASTOCYSTS MOUSE EMBRYOS IPS CELL TRANSPLANTATION LINES NEURONS CULTURE neural regeneration stem cells sheep amniotic epithelial cells isolation and culture stem cell characteristics differentiation differentiation potential reverse transcription-PCR immunofluorescence microscopy grants-supported paper neuroregeneration |
| Indexed By | CSCD |
| Language | 英语 |
| Funding Project | [National High-Tech Research and Development Program of China (863 Program)] |
| CSCD ID | CSCD:4862026 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.imr.ac.cn/handle/321006/145942 |
| Collection | 中国科学院金属研究所 |
| Affiliation | 1.Inner Mongolia Agr University, Coll Vet Med, Hohhot 010018, Inner Mongolia, Peoples R China 2.河南大学 3.中国科学院金属研究所 |
| Recommended Citation GB/T 7714 | Zhu Xuemin,Wang Xiumei,Cao Guifang,et al. Stem cell properties and neural differentiation of sheep amniotic epithelial cells[J]. NEURAL REGENERATION RESEARCH,2013,8(13):1210-1219. |
| APA | Zhu Xuemin.,Wang Xiumei.,Cao Guifang.,Liu Fengjun.,Yang Yinfeng.,...&Zhang Lingli.(2013).Stem cell properties and neural differentiation of sheep amniotic epithelial cells.NEURAL REGENERATION RESEARCH,8(13),1210-1219. |
| MLA | Zhu Xuemin,et al."Stem cell properties and neural differentiation of sheep amniotic epithelial cells".NEURAL REGENERATION RESEARCH 8.13(2013):1210-1219. |
| Files in This Item: | There are no files associated with this item. | |||||
Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.
Edit Comment