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Stem cell properties and neural differentiation of sheep amniotic epithelial cells
Alternative TitleStem cell properties and neural differentiation of sheep amniotic epithelial cells ☆
Zhu Xuemin1; Wang Xiumei1; Cao Guifang1; Liu Fengjun2; Yang Yinfeng1; Li Xiaonan3; Zhang Yuling2; Mi Yan1; Liu Junping1; Zhang Lingli1
2013
Source PublicationNEURAL REGENERATION RESEARCH
ISSN1673-5374
Volume8Issue:13Pages:1210-1219
AbstractThis study was designed to verify the stem cell properties of sheep amniotic epithelial cells and their capacity for neural differentiation. Immunofluorescence microscopy and reverse transcription-PCR revealed that the sheep amniotic epithelial cells were positive for the embryonic stem cell marker proteins SSEA-1, SSEA-3, SSEA-4, TRA-1-60 and TRA-1-81, and the totipotency-associated genes Oct-4, Sox-2 and Rex-1, but negative for Nanog. Amniotic epithelial cells expressed beta-III-tubulin, glial fibrillary acidic protein, nestin and microtubule-associated protein-2 at 28 days after induction with serum-free neurobasal-A medium containing B-27. Thus, sheep amniotic epithelial cells could differentiate into neurons expressing beta-III-tubulin and microtubule-associated protein-2, and glial-like cells expressing glial fibrillary acidic protein, under specific conditions.
Other AbstractThis study was designed to verify the stem cell properties of sheep amniotic epithelial cells and their capacity for neural differentiation. Immunofluorescence microscopy and reverse transcription-PCR revealed that the sheep amniotic epithelial cells were positive for the embryonic stem cell marker proteins SSEA-1, SSEA-3, SSEA-4, TRA-1-60 and TRA-1-81, and the totipotency-associated genes Oct-4, Sox-2 and Rex-1, but negative for Nanog. Amniotic epithelial cells expressed β-III-tubulin, glial fibrillary acidic protein, nestin and microtubule-associated protein-2 at 28 days after induction with serum-free neurobasal-A medium containing B-27. Thus, sheep amniotic epithelial cells could differentiate into neurons expressing β-III-tubulin and microtubule-associated protein-2, and glial-like cells expressing glial fibrillary acidic protein, under specific conditions.
KeywordPRIMORDIAL GERM-CELLS IN-VITRO PARKINSONS-DISEASE HUMAN BLASTOCYSTS MOUSE EMBRYOS IPS CELL TRANSPLANTATION LINES NEURONS CULTURE neural regeneration stem cells sheep amniotic epithelial cells isolation and culture stem cell characteristics differentiation differentiation potential reverse transcription-PCR immunofluorescence microscopy grants-supported paper neuroregeneration
Indexed ByCSCD
Language英语
Funding Project[National High-Tech Research and Development Program of China (863 Program)]
CSCD IDCSCD:4862026
Citation statistics
Document Type期刊论文
Identifierhttp://ir.imr.ac.cn/handle/321006/145942
Collection中国科学院金属研究所
Affiliation1.Inner Mongolia Agr University, Coll Vet Med, Hohhot 010018, Inner Mongolia, Peoples R China
2.河南大学
3.中国科学院金属研究所
Recommended Citation
GB/T 7714
Zhu Xuemin,Wang Xiumei,Cao Guifang,et al. Stem cell properties and neural differentiation of sheep amniotic epithelial cells[J]. NEURAL REGENERATION RESEARCH,2013,8(13):1210-1219.
APA Zhu Xuemin.,Wang Xiumei.,Cao Guifang.,Liu Fengjun.,Yang Yinfeng.,...&Zhang Lingli.(2013).Stem cell properties and neural differentiation of sheep amniotic epithelial cells.NEURAL REGENERATION RESEARCH,8(13),1210-1219.
MLA Zhu Xuemin,et al."Stem cell properties and neural differentiation of sheep amniotic epithelial cells".NEURAL REGENERATION RESEARCH 8.13(2013):1210-1219.
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