Stem cell properties and neural differentiation of sheep amniotic epithelial cells

IMR OpenIR

	Stem cell properties and neural differentiation of sheep amniotic epithelial cells
其他题名	Stem cell properties and neural differentiation of sheep amniotic epithelial cells ☆
	Zhu Xuemin 1; Wang Xiumei 1; Cao Guifang 1; Liu Fengjun 2; Yang Yinfeng 1; Li Xiaonan 3; Zhang Yuling 2; Mi Yan 1; Liu Junping 1; Zhang Lingli 1
	2013
发表期刊	NEURAL REGENERATION RESEARCH
ISSN	1673-5374
卷号	8 期号:13 页码:1210-1219
摘要	This study was designed to verify the stem cell properties of sheep amniotic epithelial cells and their capacity for neural differentiation. Immunofluorescence microscopy and reverse transcription-PCR revealed that the sheep amniotic epithelial cells were positive for the embryonic stem cell marker proteins SSEA-1, SSEA-3, SSEA-4, TRA-1-60 and TRA-1-81, and the totipotency-associated genes Oct-4, Sox-2 and Rex-1, but negative for Nanog. Amniotic epithelial cells expressed beta-III-tubulin, glial fibrillary acidic protein, nestin and microtubule-associated protein-2 at 28 days after induction with serum-free neurobasal-A medium containing B-27. Thus, sheep amniotic epithelial cells could differentiate into neurons expressing beta-III-tubulin and microtubule-associated protein-2, and glial-like cells expressing glial fibrillary acidic protein, under specific conditions.
其他摘要	This study was designed to verify the stem cell properties of sheep amniotic epithelial cells and their capacity for neural differentiation. Immunofluorescence microscopy and reverse transcription-PCR revealed that the sheep amniotic epithelial cells were positive for the embryonic stem cell marker proteins SSEA-1, SSEA-3, SSEA-4, TRA-1-60 and TRA-1-81, and the totipotency-associated genes Oct-4, Sox-2 and Rex-1, but negative for Nanog. Amniotic epithelial cells expressed β-III-tubulin, glial fibrillary acidic protein, nestin and microtubule-associated protein-2 at 28 days after induction with serum-free neurobasal-A medium containing B-27. Thus, sheep amniotic epithelial cells could differentiate into neurons expressing β-III-tubulin and microtubule-associated protein-2, and glial-like cells expressing glial fibrillary acidic protein, under specific conditions.
关键词	PRIMORDIAL GERM-CELLS IN-VITRO PARKINSONS-DISEASE HUMAN BLASTOCYSTS MOUSE EMBRYOS IPS CELL TRANSPLANTATION LINES NEURONS CULTURE neural regeneration stem cells sheep amniotic epithelial cells isolation and culture stem cell characteristics differentiation differentiation potential reverse transcription-PCR immunofluorescence microscopy grants-supported paper neuroregeneration
收录类别	CSCD
语种	英语
资助项目	[National High-Tech Research and Development Program of China (863 Program)]
CSCD记录号	CSCD:4862026
引用统计
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/145942
专题	中国科学院金属研究所
作者单位	1.Inner Mongolia Agr University, Coll Vet Med, Hohhot 010018, Inner Mongolia, Peoples R China 2.河南大学 3.中国科学院金属研究所
推荐引用方式 GB/T 7714	Zhu Xuemin,Wang Xiumei,Cao Guifang,et al. Stem cell properties and neural differentiation of sheep amniotic epithelial cells[J]. NEURAL REGENERATION RESEARCH,2013,8(13):1210-1219.
APA	Zhu Xuemin.,Wang Xiumei.,Cao Guifang.,Liu Fengjun.,Yang Yinfeng.,...&Zhang Lingli.(2013).Stem cell properties and neural differentiation of sheep amniotic epithelial cells.NEURAL REGENERATION RESEARCH,8(13),1210-1219.
MLA	Zhu Xuemin,et al."Stem cell properties and neural differentiation of sheep amniotic epithelial cells".NEURAL REGENERATION RESEARCH 8.13(2013):1210-1219.