| Determination of active ingredients of hawthorn by capillary electrophoresis with electrochemical detection | |
| Alternative Title | Determination of Active Ingredients of Hawthorn by Capillary Electrophoresis with Electrochemical Detection |
| Tang ZhuXing1; Zeng YiKun1; Zhou Yun1; Zang ShuLiang1; He PinGang1; Fang YuZhi1 | |
| 2006 | |
| Source Publication | CHINESE JOURNAL OF CHEMISTRY
 |
| ISSN | 1001-604X |
| Volume | 24Issue:10Pages:1378-1383 |
| Abstract | A method based on capillary electrophoresis with electrochemical detection has been developed for the separation and determination of epicatechin, kaempferol, chlorogenic acid, 4-hydroxybenzoic acid, quercetin and protocatechuic acid in hawthorn for the first time. The effects of working electrode potential, pH and concentration of running buffer, separation voltage and injection time on CE-ED were investigated. Under the optimum conditions, the analytes could be separated in a 60 mmol(.)L(-1) borate buffer (pH 8.7) within 21 min. A 300 pm diameter carbon disk electrode has a good response at +0.95 V (vs. SCE) for all analytes. The response was linear over three orders of magnitude with detection limits (S/N=3) ranging from 3 x 10(-8) to 2 x 10(-7) g(.)mL(-1) for the analytes. The method has been successfully applied to the analysis of real sample, with satisfactory results. |
| Other Abstract | A method based on capillary electrophoresis with electrochemical detection has been developed for the separation and determination of epicatechin, kaempferol, chlorogenic acid, 4-hydroxybenzoic acid, quercetin and protocatechuic acid in hawthorn for the first time. The effects of working electrode potential, pH and concentration of running buffer, separation voltage and injection time on CE-ED were investigated. Under the optimum conditions, the analytes could be separated in a 60 mmoloL-1 borate buffer （pH 8.7） within 21 rain. A 300 lam diameter carbon disk electrode has a good response at ＋0.95 V （vs, SCE） for all analytes. The response was linear over three orders of magnitude with detection limits （S/N=3） ranging from 3 × 10~(-8) to 2 × 10~(-7) g·mL~(-1) for the analytes. The method has been successfully applied to the analysis of real sample, with satisfactory results. |
| Keyword | FLAVONOIDS ANTIOXIDANT COMPONENTS WINES capillary electrophoresis electrochemical detection hawthorn |
| Indexed By | CSCD |
| Language | 英语 |
| CSCD ID | CSCD:2727174 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.imr.ac.cn/handle/321006/146115 |
| Collection | 中国科学院金属研究所 |
| Affiliation | 1.E China Normal University 2.中国科学院金属研究所 3.辽宁大学 |
| Recommended Citation GB/T 7714 | Tang ZhuXing,Zeng YiKun,Zhou Yun,et al. Determination of active ingredients of hawthorn by capillary electrophoresis with electrochemical detection[J]. CHINESE JOURNAL OF CHEMISTRY,2006,24(10):1378-1383. |
| APA | Tang ZhuXing,Zeng YiKun,Zhou Yun,Zang ShuLiang,He PinGang,&Fang YuZhi.(2006).Determination of active ingredients of hawthorn by capillary electrophoresis with electrochemical detection.CHINESE JOURNAL OF CHEMISTRY,24(10),1378-1383. |
| MLA | Tang ZhuXing,et al."Determination of active ingredients of hawthorn by capillary electrophoresis with electrochemical detection".CHINESE JOURNAL OF CHEMISTRY 24.10(2006):1378-1383. |
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