Determination of active ingredients of hawthorn by capillary electrophoresis with electrochemical detection

IMR OpenIR

	Determination of active ingredients of hawthorn by capillary electrophoresis with electrochemical detection
其他题名	Determination of Active Ingredients of Hawthorn by Capillary Electrophoresis with Electrochemical Detection
	Tang ZhuXing 1; Zeng YiKun 1; Zhou Yun 1; Zang ShuLiang 1; He PinGang 1; Fang YuZhi 1
	2006
发表期刊	CHINESE JOURNAL OF CHEMISTRY
ISSN	1001-604X
卷号	24 期号:10 页码:1378-1383
摘要	A method based on capillary electrophoresis with electrochemical detection has been developed for the separation and determination of epicatechin, kaempferol, chlorogenic acid, 4-hydroxybenzoic acid, quercetin and protocatechuic acid in hawthorn for the first time. The effects of working electrode potential, pH and concentration of running buffer, separation voltage and injection time on CE-ED were investigated. Under the optimum conditions, the analytes could be separated in a 60 mmol(.)L(-1) borate buffer (pH 8.7) within 21 min. A 300 pm diameter carbon disk electrode has a good response at +0.95 V (vs. SCE) for all analytes. The response was linear over three orders of magnitude with detection limits (S/N=3) ranging from 3 x 10(-8) to 2 x 10(-7) g(.)mL(-1) for the analytes. The method has been successfully applied to the analysis of real sample, with satisfactory results.
其他摘要	A method based on capillary electrophoresis with electrochemical detection has been developed for the separation and determination of epicatechin, kaempferol, chlorogenic acid, 4-hydroxybenzoic acid, quercetin and protocatechuic acid in hawthorn for the first time. The effects of working electrode potential, pH and concentration of running buffer, separation voltage and injection time on CE-ED were investigated. Under the optimum conditions, the analytes could be separated in a 60 mmoloL-1 borate buffer （pH 8.7） within 21 rain. A 300 lam diameter carbon disk electrode has a good response at ＋0.95 V （vs, SCE） for all analytes. The response was linear over three orders of magnitude with detection limits （S/N=3） ranging from 3 × 10~(-8) to 2 × 10~(-7) g·mL~(-1) for the analytes. The method has been successfully applied to the analysis of real sample, with satisfactory results.
关键词	FLAVONOIDS ANTIOXIDANT COMPONENTS WINES capillary electrophoresis electrochemical detection hawthorn
收录类别	CSCD
语种	英语
CSCD记录号	CSCD:2727174
引用统计
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/146115
专题	中国科学院金属研究所
作者单位	1.E China Normal University 2.中国科学院金属研究所 3.辽宁大学
推荐引用方式 GB/T 7714	Tang ZhuXing,Zeng YiKun,Zhou Yun,et al. Determination of active ingredients of hawthorn by capillary electrophoresis with electrochemical detection[J]. CHINESE JOURNAL OF CHEMISTRY,2006,24(10):1378-1383.
APA	Tang ZhuXing,Zeng YiKun,Zhou Yun,Zang ShuLiang,He PinGang,&Fang YuZhi.(2006).Determination of active ingredients of hawthorn by capillary electrophoresis with electrochemical detection.CHINESE JOURNAL OF CHEMISTRY,24(10),1378-1383.
MLA	Tang ZhuXing,et al."Determination of active ingredients of hawthorn by capillary electrophoresis with electrochemical detection".CHINESE JOURNAL OF CHEMISTRY 24.10(2006):1378-1383.