Improvement of porous electro-conductive membrane preparation and its application in sample injection pre-concentration in capillary electrophoresis of proteins

IMR OpenIR

	Improvement of porous electro-conductive membrane preparation and its application in sample injection pre-concentration in capillary electrophoresis of proteins
其他题名	Improvement of Porous Electro-conductive Membrane Preparation and Its Application in Sample Injection Pre-concentration in Capillary Electrophoresis of Proteins
	Wang YaRong; Li JingJing; Wu ZhiYong; Fan JunGang; Fang Fang
	2008
发表期刊	CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
ISSN	0253-3820
卷号	36 期号:7 页码:879-884
摘要	The success rate of porous conductive membrane preparation on fused silica capillary was greatly improved by control of voltage bias and electroosmosis effect. Basic buffer media was filled into the column, and a direct curvent voltage was applied between the capillary inlet and the etching reservoir during the etching process. The etching was stopped due to electroosmosis effect when electro-conductivity of the etching membrane occurred, and over etching was automatically avoided. The conductive membrane prepared was successfully used in on-line sample electro injection pre-concentration and capillary electrophoresis separation of model proteins of lysozyme and bovine serum albumin in 0.05 mol/L pH 2.50 Tris-H, PO, with 2% - 4% dextran, and a concentration factor close to the theoretical value was obtained. The difference of concentration factors for basic and acidic proteins was discussed. Detection limits for the model proteins were decreased to 1 x 10(-11) mol/L with a routinely used photometric detector (214 nm wavelegnth) by electro-injection pre-concentration for 30 min.
关键词	ZONE-ELECTROPHORESIS ELECTROKINETIC CHROMATOGRAPHY SENSITIVITY ENHANCEMENT ETCHED JOINT STACKING SYSTEM SEPARATION INTERFACE PEPTIDES capillary electrophoresis porous conductive membrane injection pre-concentration lysozyme bovine serum albumin ultraviolet detection
收录类别	CSCD
语种	英语
CSCD记录号	CSCD:3333224
引用统计	被引频次：1[CSCD] [CSCD记录]
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/146268
专题	中国科学院金属研究所
作者单位	中国科学院金属研究所
推荐引用方式 GB/T 7714	Wang YaRong,Li JingJing,Wu ZhiYong,et al. Improvement of porous electro-conductive membrane preparation and its application in sample injection pre-concentration in capillary electrophoresis of proteins[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2008,36(7):879-884.
APA	Wang YaRong,Li JingJing,Wu ZhiYong,Fan JunGang,&Fang Fang.(2008).Improvement of porous electro-conductive membrane preparation and its application in sample injection pre-concentration in capillary electrophoresis of proteins.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,36(7),879-884.
MLA	Wang YaRong,et al."Improvement of porous electro-conductive membrane preparation and its application in sample injection pre-concentration in capillary electrophoresis of proteins".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 36.7(2008):879-884.