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Improvement of porous electro-conductive membrane preparation and its application in sample injection pre-concentration in capillary electrophoresis of proteins
Alternative TitleImprovement of Porous Electro-conductive Membrane Preparation and Its Application in Sample Injection Pre-concentration in Capillary Electrophoresis of Proteins
Wang YaRong; Li JingJing; Wu ZhiYong; Fan JunGang; Fang Fang
2008
Source PublicationCHINESE JOURNAL OF ANALYTICAL CHEMISTRY
ISSN0253-3820
Volume36Issue:7Pages:879-884
AbstractThe success rate of porous conductive membrane preparation on fused silica capillary was greatly improved by control of voltage bias and electroosmosis effect. Basic buffer media was filled into the column, and a direct curvent voltage was applied between the capillary inlet and the etching reservoir during the etching process. The etching was stopped due to electroosmosis effect when electro-conductivity of the etching membrane occurred, and over etching was automatically avoided. The conductive membrane prepared was successfully used in on-line sample electro injection pre-concentration and capillary electrophoresis separation of model proteins of lysozyme and bovine serum albumin in 0.05 mol/L pH 2.50 Tris-H, PO, with 2% - 4% dextran, and a concentration factor close to the theoretical value was obtained. The difference of concentration factors for basic and acidic proteins was discussed. Detection limits for the model proteins were decreased to 1 x 10(-11) mol/L with a routinely used photometric detector (214 nm wavelegnth) by electro-injection pre-concentration for 30 min.
KeywordZONE-ELECTROPHORESIS ELECTROKINETIC CHROMATOGRAPHY SENSITIVITY ENHANCEMENT ETCHED JOINT STACKING SYSTEM SEPARATION INTERFACE PEPTIDES capillary electrophoresis porous conductive membrane injection pre-concentration lysozyme bovine serum albumin ultraviolet detection
Indexed ByCSCD
Language英语
CSCD IDCSCD:3333224
Citation statistics
Cited Times:1[CSCD]   [CSCD Record]
Document Type期刊论文
Identifierhttp://ir.imr.ac.cn/handle/321006/146268
Collection中国科学院金属研究所
Affiliation中国科学院金属研究所
Recommended Citation
GB/T 7714
Wang YaRong,Li JingJing,Wu ZhiYong,et al. Improvement of porous electro-conductive membrane preparation and its application in sample injection pre-concentration in capillary electrophoresis of proteins[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2008,36(7):879-884.
APA Wang YaRong,Li JingJing,Wu ZhiYong,Fan JunGang,&Fang Fang.(2008).Improvement of porous electro-conductive membrane preparation and its application in sample injection pre-concentration in capillary electrophoresis of proteins.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,36(7),879-884.
MLA Wang YaRong,et al."Improvement of porous electro-conductive membrane preparation and its application in sample injection pre-concentration in capillary electrophoresis of proteins".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 36.7(2008):879-884.
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