Effect of insulin on oxygen free radicals and oxidative phosphorylation in liver mitochondria of diabetic rats

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	Effect of insulin on oxygen free radicals and oxidative phosphorylation in liver mitochondria of diabetic rats
其他题名	Effect of insulin on oxygen free radicals and oxidative phosphorylation in liver mitochondria of diabetic rats
	Huang Q 1; Shao L 1; Jiang H 1; Miao ZC 1; Shi QD 1; Liu SS 1
	2001
发表期刊	ACTA PHARMACOLOGICA SINICA
ISSN	1671-4083
卷号	22 期号:5 页码:455-458
摘要	AIM: To observe the effects of insulin on liver mitochondrial respiratory function, activity of H+-ATPase, and superoxide anion free radicals production in diabetic rats. METHODS: Rats were injected iv with alloxan 40 mg/kg to induce diabetes. The liver mitochondrial respiratory function was assayed by measurement of oxygen consumption using a Clark oxygen electrode. Superoxide anion production was assayed using chemiluminescence method. Activities of HC-ATPase were measured by luciferin-luciferase system and inorganic phosphorus's method. RESULTS: Insulin 1 U/kg sc daily for 9 weeks improved oxidative phosphorylation, respiratory rate state 3 (P 0.05). In the insulin group, synthesis activity of H+-ATPase was obviously increased (P < 0.05) acid hydrolytic activity of H+-ATPase was remarkably decreased (P < 0. 01), compared with the diabetes group. Insulin 1 U/kg for 9 weeks apparently decreased the production of O-2(.-)(P < 0. 01) in liver mitochondria of diabetic rats. CONCLUSION: Insulin can prevent the injury from superoxide anion in liver mitochondria, and improve the function of the liver mitochondria oxidative phosphorylation.
关键词	insulin experimental diabetes mellitus mitochondria free radicals oxidative phosphorylation
收录类别	CSCD
语种	英语
CSCD记录号	CSCD:515825
引用统计	被引频次：1[CSCD] [CSCD记录]
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/146313
专题	中国科学院金属研究所
作者单位	1.中国科学院 2.中国科学院金属研究所
推荐引用方式 GB/T 7714	Huang Q,Shao L,Jiang H,et al. Effect of insulin on oxygen free radicals and oxidative phosphorylation in liver mitochondria of diabetic rats[J]. ACTA PHARMACOLOGICA SINICA,2001,22(5):455-458.
APA	Huang Q,Shao L,Jiang H,Miao ZC,Shi QD,&Liu SS.(2001).Effect of insulin on oxygen free radicals and oxidative phosphorylation in liver mitochondria of diabetic rats.ACTA PHARMACOLOGICA SINICA,22(5),455-458.
MLA	Huang Q,et al."Effect of insulin on oxygen free radicals and oxidative phosphorylation in liver mitochondria of diabetic rats".ACTA PHARMACOLOGICA SINICA 22.5(2001):455-458.