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Preparation of GSH Capped CdSe/CdS Core-Shell QDs and Labeling of Human T-Lymphocyte
Alternative TitlePreparation of GSH Capped CdSe/CdS Core-Shell QDs and Labeling of Human T-Lymphocyte
Dong Wei1; Ge Xin2; Wang Xuanyi2; Xu Shukun1
2010
Source PublicationSPECTROSCOPY AND SPECTRAL ANALYSIS
ISSN1000-0593
Volume30Issue:1Pages:118-122
AbstractTwo kinds of L-glutathione capped highly fluorescent CdSe/CdS core-shell quantum dots (QDs) emitting green and orange fluorescence at 350 nm excitation were firstly prepared by an aqueous approach and used as fluorescent labels, to link mouse anti-human CD3 which was expressed on human T-lymphocyte. UV-Vis absorption and fluorescence emission spectra of the as-prepared CdSe/CdS core-shell QDs were studied. Compared with the CdSe QDs, a remarkable enhancement in the emission intensity and a red shift of emission wavelength of CdSe/CdS core-shell QDs was observed for the two kinds of QDs emitting green and orange fluorescence. The TEM results showed that the as prepared CdSe and CdSe/CdS QDs dispersed well in aqueous solution, and their shape was approximately spherical, and the CdSe/CdS QDs nano particles emitting green fluorescence are of about 5 run in diameter. The two kinds of CdSe/CdS QDs were linked with mouse anti-human CD3 to image human T-lymphocyte. The fluorescent microscopical images of human T-lymphocyte labeled with CdSe/CdS QDs-CD3 and FITC-CD3 demonstrated that the fluorescent CdSe/CdS QDs exhibited much better photo stability and brighter fluorescence than FITC, showing a good application potential in the immuno-labeling of cells.
KeywordQUANTUM DOTS SEMICONDUCTOR NANOCRYSTALS CELLS Aqueous CdSe/CdS core-shell QDs L-glutathione Immunolabeling Human T-lymphocyte
Indexed ByCSCD
Language英语
CSCD IDCSCD:3825007
Citation statistics
Cited Times:3[CSCD]   [CSCD Record]
Document Type期刊论文
Identifierhttp://ir.imr.ac.cn/handle/321006/147307
Collection中国科学院金属研究所
Affiliation1.东北大学
2.中国科学院金属研究所
Recommended Citation
GB/T 7714
Dong Wei,Ge Xin,Wang Xuanyi,et al. Preparation of GSH Capped CdSe/CdS Core-Shell QDs and Labeling of Human T-Lymphocyte[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS,2010,30(1):118-122.
APA Dong Wei,Ge Xin,Wang Xuanyi,&Xu Shukun.(2010).Preparation of GSH Capped CdSe/CdS Core-Shell QDs and Labeling of Human T-Lymphocyte.SPECTROSCOPY AND SPECTRAL ANALYSIS,30(1),118-122.
MLA Dong Wei,et al."Preparation of GSH Capped CdSe/CdS Core-Shell QDs and Labeling of Human T-Lymphocyte".SPECTROSCOPY AND SPECTRAL ANALYSIS 30.1(2010):118-122.
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