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Tanshinone IIA inhibits endothelin-1 production in TNF-α-induced brain microvascular endothelial cells through suppression of endothelin-converting enzyme-1 synthesis
Alternative TitleTanshinone IIA inhibits endothelin-1 production in TNF-α-induced brain microvascular endothelial cells through suppression of endothelin-converting enzyme-1 synthesis
Chao TANG1; Anhua WU1; Hongli XUE2; Yunjie WANG1
2007
Source PublicationActa Pharmacologica Sinica
ISSN1671-4083
Volume28Issue:8Pages:1116-1122
AbstractTo investigate the effects of tanshinone IIA (Tan IIA) on the regulation of the production of endothelin (ET)-1 (including large ET-1), mRNA levels of ET-1, endothelin-converting enzyme-1 (ECE-1), endothelin-A receptor (ET_A) and endothelin-B receptor (ETB) induced by TNF-α in rat brain microvascular endothelial cells (BMVEC). Methods: The ET-1 release (including large ET-1) into the culture medium was determined by enzyme immunoassay. The levels of ET-1, ECE-1, ET_A, and ETB mRNA were measured by RT-PCR. Endothelin receptor binding was also tested. Results: The induction of ET-1 release by TNF-α from cultured BMVEC was dose-dependently reduced by Tan IIA, but large ET-1 levels progressively increased in response to Tan IIA; the mRNA expression of ET-1 was unaffected. Tan IIA also caused a decrease in ET_A receptor mRNA and ECE-1 expression in a dose-dependent manner. Endothelin receptor binding was unaltered in BMVEC stimulated with TNF-α alone or a combination of TNF-α and Tan IIA. Conclusion: These findings suggest that Tan IIA may inhibit ET-1 production in TNF-α-induced BMVEC through the suppression of ECE-1 synthesis.
Other AbstractTo investigate the effects of tanshinone IIA (Tan IIA) on the regulation of the production of endothelin (ET)-1 (including large ET-1), mRNA levels of ET-1, endothelin-converting enzyme-1 (ECE-1), endothelin-A receptor (ET_A) and endothelin-B receptor (ETB) induced by TNF-α in rat brain microvascular endothelial cells (BMVEC). Methods: The ET-1 release (including large ET-1) into the culture medium was determined by enzyme immunoassay. The levels of ET-1, ECE-1, ET_A, and ETB mRNA were measured by RT-PCR. Endothelin receptor binding was also tested. Results: The induction of ET-1 release by TNF-α from cultured BMVEC was dose-dependently reduced by Tan IIA, but large ET-1 levels progressively increased in response to Tan IIA; the mRNA expression of ET-1 was unaffected. Tan IIA also caused a decrease in ET_A receptor mRNA and ECE-1 expression in a dose-dependent manner. Endothelin receptor binding was unaltered in BMVEC stimulated with TNF-α alone or a combination of TNF-α and Tan IIA. Conclusion: These findings suggest that Tan IIA may inhibit ET-1 production in TNF-α-induced BMVEC through the suppression of ECE-1 synthesis.
Keywordtanshinone IIA endothelin-1 TNF-α brain microvascular endothelial cells
Indexed ByCSCD
Language英语
CSCD IDCSCD:3088142
Citation statistics
Document Type期刊论文
Identifierhttp://ir.imr.ac.cn/handle/321006/147357
Collection中国科学院金属研究所
Affiliation1.中国医科大学
2.中国科学院金属研究所
Recommended Citation
GB/T 7714
Chao TANG,Anhua WU,Hongli XUE,et al. Tanshinone IIA inhibits endothelin-1 production in TNF-α-induced brain microvascular endothelial cells through suppression of endothelin-converting enzyme-1 synthesis[J]. Acta Pharmacologica Sinica,2007,28(8):1116-1122.
APA Chao TANG,Anhua WU,Hongli XUE,&Yunjie WANG.(2007).Tanshinone IIA inhibits endothelin-1 production in TNF-α-induced brain microvascular endothelial cells through suppression of endothelin-converting enzyme-1 synthesis.Acta Pharmacologica Sinica,28(8),1116-1122.
MLA Chao TANG,et al."Tanshinone IIA inhibits endothelin-1 production in TNF-α-induced brain microvascular endothelial cells through suppression of endothelin-converting enzyme-1 synthesis".Acta Pharmacologica Sinica 28.8(2007):1116-1122.
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