Tanshinone IIA inhibits endothelin-1 production in TNF-α-induced brain microvascular endothelial cells through suppression of endothelin-converting enzyme-1 synthesis

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	Tanshinone IIA inhibits endothelin-1 production in TNF-α-induced brain microvascular endothelial cells through suppression of endothelin-converting enzyme-1 synthesis
其他题名	Tanshinone IIA inhibits endothelin-1 production in TNF-α-induced brain microvascular endothelial cells through suppression of endothelin-converting enzyme-1 synthesis
	Chao TANG 1; Anhua WU 1; Hongli XUE 2; Yunjie WANG 1
	2007
发表期刊	Acta Pharmacologica Sinica
ISSN	1671-4083
卷号	28 期号:8 页码:1116-1122
摘要	To investigate the effects of tanshinone IIA (Tan IIA) on the regulation of the production of endothelin (ET)-1 (including large ET-1), mRNA levels of ET-1, endothelin-converting enzyme-1 (ECE-1), endothelin-A receptor (ET_A) and endothelin-B receptor (ETB) induced by TNF-α in rat brain microvascular endothelial cells (BMVEC). Methods: The ET-1 release (including large ET-1) into the culture medium was determined by enzyme immunoassay. The levels of ET-1, ECE-1, ET_A, and ETB mRNA were measured by RT-PCR. Endothelin receptor binding was also tested. Results: The induction of ET-1 release by TNF-α from cultured BMVEC was dose-dependently reduced by Tan IIA, but large ET-1 levels progressively increased in response to Tan IIA; the mRNA expression of ET-1 was unaffected. Tan IIA also caused a decrease in ET_A receptor mRNA and ECE-1 expression in a dose-dependent manner. Endothelin receptor binding was unaltered in BMVEC stimulated with TNF-α alone or a combination of TNF-α and Tan IIA. Conclusion: These findings suggest that Tan IIA may inhibit ET-1 production in TNF-α-induced BMVEC through the suppression of ECE-1 synthesis.
其他摘要	To investigate the effects of tanshinone IIA (Tan IIA) on the regulation of the production of endothelin (ET)-1 (including large ET-1), mRNA levels of ET-1, endothelin-converting enzyme-1 (ECE-1), endothelin-A receptor (ET_A) and endothelin-B receptor (ETB) induced by TNF-α in rat brain microvascular endothelial cells (BMVEC). Methods: The ET-1 release (including large ET-1) into the culture medium was determined by enzyme immunoassay. The levels of ET-1, ECE-1, ET_A, and ETB mRNA were measured by RT-PCR. Endothelin receptor binding was also tested. Results: The induction of ET-1 release by TNF-α from cultured BMVEC was dose-dependently reduced by Tan IIA, but large ET-1 levels progressively increased in response to Tan IIA; the mRNA expression of ET-1 was unaffected. Tan IIA also caused a decrease in ET_A receptor mRNA and ECE-1 expression in a dose-dependent manner. Endothelin receptor binding was unaltered in BMVEC stimulated with TNF-α alone or a combination of TNF-α and Tan IIA. Conclusion: These findings suggest that Tan IIA may inhibit ET-1 production in TNF-α-induced BMVEC through the suppression of ECE-1 synthesis.
关键词	tanshinone IIA endothelin-1 TNF-α brain microvascular endothelial cells
收录类别	CSCD
语种	英语
CSCD记录号	CSCD:3088142
引用统计
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/147357
专题	中国科学院金属研究所
作者单位	1.中国医科大学 2.中国科学院金属研究所
推荐引用方式 GB/T 7714	Chao TANG,Anhua WU,Hongli XUE,et al. Tanshinone IIA inhibits endothelin-1 production in TNF-α-induced brain microvascular endothelial cells through suppression of endothelin-converting enzyme-1 synthesis[J]. Acta Pharmacologica Sinica,2007,28(8):1116-1122.
APA	Chao TANG,Anhua WU,Hongli XUE,&Yunjie WANG.(2007).Tanshinone IIA inhibits endothelin-1 production in TNF-α-induced brain microvascular endothelial cells through suppression of endothelin-converting enzyme-1 synthesis.Acta Pharmacologica Sinica,28(8),1116-1122.
MLA	Chao TANG,et al."Tanshinone IIA inhibits endothelin-1 production in TNF-α-induced brain microvascular endothelial cells through suppression of endothelin-converting enzyme-1 synthesis".Acta Pharmacologica Sinica 28.8(2007):1116-1122.