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Analysis of expressed sequence tags (ESTs) from a normalized cDNA library and isolation of EST simple sequence repeats from the invasive cotton mealybug phenacoccus solenopsis
Alternative TitleAnalysis of expressed sequence tags (ESTs) from a normalized cDNA library and isolation of EST simple sequence repeats from the invasive cotton mealybug Phenacoccus solenopsis
Li Hui1; Lang KunLing1; Fu HaiBin2; Shen ChangPeng1; Wan FangHao1; Chu Dong1
2015
Source PublicationINSECT SCIENCE
ISSN1672-9609
Volume22Issue:6Pages:761-767
AbstractThe cotton mealybug, Phenacoccus solenopsis Tinsley, is a serious and invasive pest. At present, genetic resources for studying P. solenopsis are limited, and this negatively affects genetic research on the organism and, consequently, translational work to improve management of this pest. In the present study, expressed sequence tags (ESTs) were analyzed from a normalized complementary DNA library of P. solenopsis. In addition, EST-derived microsatellite loci (also known as simple sequence repeats or SSRs) were isolated and characterized. A total of 1107 high-quality ESTs were acquired from the library. Clustering and assembly analysis resulted in 785 unigenes, which were classified functionally into 23 categories according to the Gene Ontology database. Seven EST-based SSR markers were developed in this study and are expected to be useful in characterizing how this invasive species was introduced, as well as providing insights into its genetic microevolution.
Other AbstractThe cottonmealybug, Phenacoccus solenopsis Tinsley, is a serious and invasive pest. At present, genetic resources for studying P. solenopsis are limited, and this negatively affects genetic research on the organism and, consequently, translational work to improve management of this pest. In the present study, expressed sequence tags (ESTs) were analyzed from a normalized complementary DNA library of P. solenopsis. In addition, EST-derived microsatellite loci (also known as simple sequence repeats or SSRs) were isolated and characterized. A total of 1107 high-quality ESTs were acquired from the library. Clustering and assembly analysis resulted in 785 unigenes, which were classified functionally into 23 categories according to the Gene Ontology database. Seven EST-based SSR markers were developed in this study and are expected to be useful in characterizing how this invasive species was introduced, as well as providing insights into its genetic microevolution.
KeywordTINSLEY HEMIPTERA PSEUDOCOCCIDAE RNA INTERFERENCE HYPERTREHALOSEMIC HORMONE GENE COCKROACH PEST expressed sequence tags microsatellite marker normalized cDNA library Phenacoccus solenopsis
Indexed ByCSCD
Language英语
Funding Project[National Science and Technology Support Program] ; [Science and Technology Development Planning Program of Qingdao] ; [Taishan Mountain Scholar Constructive Engineering Foundation of Shandong] ; [Shandong Modern Agricultural Technology & Industry System]
CSCD IDCSCD:5582479
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Document Type期刊论文
Identifierhttp://ir.imr.ac.cn/handle/321006/147614
Collection中国科学院金属研究所
Affiliation1.黑龙江省环境保护科学研究院
2.中国科学院金属研究所
3.Chinese Acad Agr Sci, Institute Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China Institute Plant Protect State Key Lab Biol Plant Dis & Insect Pests
Recommended Citation
GB/T 7714
Li Hui,Lang KunLing,Fu HaiBin,et al. Analysis of expressed sequence tags (ESTs) from a normalized cDNA library and isolation of EST simple sequence repeats from the invasive cotton mealybug phenacoccus solenopsis[J]. INSECT SCIENCE,2015,22(6):761-767.
APA Li Hui,Lang KunLing,Fu HaiBin,Shen ChangPeng,Wan FangHao,&Chu Dong.(2015).Analysis of expressed sequence tags (ESTs) from a normalized cDNA library and isolation of EST simple sequence repeats from the invasive cotton mealybug phenacoccus solenopsis.INSECT SCIENCE,22(6),761-767.
MLA Li Hui,et al."Analysis of expressed sequence tags (ESTs) from a normalized cDNA library and isolation of EST simple sequence repeats from the invasive cotton mealybug phenacoccus solenopsis".INSECT SCIENCE 22.6(2015):761-767.
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