| Analysis of expressed sequence tags (ESTs) from a normalized cDNA library and isolation of EST simple sequence repeats from the invasive cotton mealybug phenacoccus solenopsis | |
| Alternative Title | Analysis of expressed sequence tags (ESTs) from a normalized cDNA library and isolation of EST simple sequence repeats from the invasive cotton mealybug Phenacoccus solenopsis |
| Li Hui1; Lang KunLing1; Fu HaiBin2; Shen ChangPeng1; Wan FangHao1; Chu Dong1 | |
| 2015 | |
| Source Publication | INSECT SCIENCE
 |
| ISSN | 1672-9609 |
| Volume | 22Issue:6Pages:761-767 |
| Abstract | The cotton mealybug, Phenacoccus solenopsis Tinsley, is a serious and invasive pest. At present, genetic resources for studying P. solenopsis are limited, and this negatively affects genetic research on the organism and, consequently, translational work to improve management of this pest. In the present study, expressed sequence tags (ESTs) were analyzed from a normalized complementary DNA library of P. solenopsis. In addition, EST-derived microsatellite loci (also known as simple sequence repeats or SSRs) were isolated and characterized. A total of 1107 high-quality ESTs were acquired from the library. Clustering and assembly analysis resulted in 785 unigenes, which were classified functionally into 23 categories according to the Gene Ontology database. Seven EST-based SSR markers were developed in this study and are expected to be useful in characterizing how this invasive species was introduced, as well as providing insights into its genetic microevolution. |
| Other Abstract | The cottonmealybug, Phenacoccus solenopsis Tinsley, is a serious and invasive pest. At present, genetic resources for studying P. solenopsis are limited, and this negatively affects genetic research on the organism and, consequently, translational work to improve management of this pest. In the present study, expressed sequence tags (ESTs) were analyzed from a normalized complementary DNA library of P. solenopsis. In addition, EST-derived microsatellite loci (also known as simple sequence repeats or SSRs) were isolated and characterized. A total of 1107 high-quality ESTs were acquired from the library. Clustering and assembly analysis resulted in 785 unigenes, which were classified functionally into 23 categories according to the Gene Ontology database. Seven EST-based SSR markers were developed in this study and are expected to be useful in characterizing how this invasive species was introduced, as well as providing insights into its genetic microevolution. |
| Keyword | TINSLEY HEMIPTERA PSEUDOCOCCIDAE RNA INTERFERENCE HYPERTREHALOSEMIC HORMONE GENE COCKROACH PEST expressed sequence tags microsatellite marker normalized cDNA library Phenacoccus solenopsis |
| Indexed By | CSCD |
| Language | 英语 |
| Funding Project | [National Science and Technology Support Program] ; [Science and Technology Development Planning Program of Qingdao] ; [Taishan Mountain Scholar Constructive Engineering Foundation of Shandong] ; [Shandong Modern Agricultural Technology & Industry System] |
| CSCD ID | CSCD:5582479 |
| Citation statistics |
Cited Times:3[CSCD]
[CSCD Record]
|
| Document Type | 期刊论文 |
| Identifier | http://ir.imr.ac.cn/handle/321006/147614 |
| Collection | 中国科学院金属研究所 |
| Affiliation | 1.黑龙江省环境保护科学研究院 2.中国科学院金属研究所 3.Chinese Acad Agr Sci, Institute Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China Institute Plant Protect State Key Lab Biol Plant Dis & Insect Pests |
| Recommended Citation GB/T 7714 | Li Hui,Lang KunLing,Fu HaiBin,et al. Analysis of expressed sequence tags (ESTs) from a normalized cDNA library and isolation of EST simple sequence repeats from the invasive cotton mealybug phenacoccus solenopsis[J]. INSECT SCIENCE,2015,22(6):761-767. |
| APA | Li Hui,Lang KunLing,Fu HaiBin,Shen ChangPeng,Wan FangHao,&Chu Dong.(2015).Analysis of expressed sequence tags (ESTs) from a normalized cDNA library and isolation of EST simple sequence repeats from the invasive cotton mealybug phenacoccus solenopsis.INSECT SCIENCE,22(6),761-767. |
| MLA | Li Hui,et al."Analysis of expressed sequence tags (ESTs) from a normalized cDNA library and isolation of EST simple sequence repeats from the invasive cotton mealybug phenacoccus solenopsis".INSECT SCIENCE 22.6(2015):761-767. |
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