Analysis of expressed sequence tags (ESTs) from a normalized cDNA library and isolation of EST simple sequence repeats from the invasive cotton mealybug phenacoccus solenopsis

IMR OpenIR

	Analysis of expressed sequence tags (ESTs) from a normalized cDNA library and isolation of EST simple sequence repeats from the invasive cotton mealybug phenacoccus solenopsis
其他题名	Analysis of expressed sequence tags (ESTs) from a normalized cDNA library and isolation of EST simple sequence repeats from the invasive cotton mealybug Phenacoccus solenopsis
	Li Hui 1; Lang KunLing 1; Fu HaiBin 2; Shen ChangPeng 1; Wan FangHao 1; Chu Dong 1
	2015
发表期刊	INSECT SCIENCE
ISSN	1672-9609
卷号	22 期号:6 页码:761-767
摘要	The cotton mealybug, Phenacoccus solenopsis Tinsley, is a serious and invasive pest. At present, genetic resources for studying P. solenopsis are limited, and this negatively affects genetic research on the organism and, consequently, translational work to improve management of this pest. In the present study, expressed sequence tags (ESTs) were analyzed from a normalized complementary DNA library of P. solenopsis. In addition, EST-derived microsatellite loci (also known as simple sequence repeats or SSRs) were isolated and characterized. A total of 1107 high-quality ESTs were acquired from the library. Clustering and assembly analysis resulted in 785 unigenes, which were classified functionally into 23 categories according to the Gene Ontology database. Seven EST-based SSR markers were developed in this study and are expected to be useful in characterizing how this invasive species was introduced, as well as providing insights into its genetic microevolution.
其他摘要	The cottonmealybug, Phenacoccus solenopsis Tinsley, is a serious and invasive pest. At present, genetic resources for studying P. solenopsis are limited, and this negatively affects genetic research on the organism and, consequently, translational work to improve management of this pest. In the present study, expressed sequence tags (ESTs) were analyzed from a normalized complementary DNA library of P. solenopsis. In addition, EST-derived microsatellite loci (also known as simple sequence repeats or SSRs) were isolated and characterized. A total of 1107 high-quality ESTs were acquired from the library. Clustering and assembly analysis resulted in 785 unigenes, which were classified functionally into 23 categories according to the Gene Ontology database. Seven EST-based SSR markers were developed in this study and are expected to be useful in characterizing how this invasive species was introduced, as well as providing insights into its genetic microevolution.
关键词	TINSLEY HEMIPTERA PSEUDOCOCCIDAE RNA INTERFERENCE HYPERTREHALOSEMIC HORMONE GENE COCKROACH PEST expressed sequence tags microsatellite marker normalized cDNA library Phenacoccus solenopsis
收录类别	CSCD
语种	英语
资助项目	[National Science and Technology Support Program] ; [Science and Technology Development Planning Program of Qingdao] ; [Taishan Mountain Scholar Constructive Engineering Foundation of Shandong] ; [Shandong Modern Agricultural Technology & Industry System]
CSCD记录号	CSCD:5582479
引用统计	被引频次：3[CSCD] [CSCD记录]
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/147614
专题	中国科学院金属研究所
作者单位	1.黑龙江省环境保护科学研究院 2.中国科学院金属研究所 3.Chinese Acad Agr Sci, Institute Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China Institute Plant Protect State Key Lab Biol Plant Dis & Insect Pests
推荐引用方式 GB/T 7714	Li Hui,Lang KunLing,Fu HaiBin,et al. Analysis of expressed sequence tags (ESTs) from a normalized cDNA library and isolation of EST simple sequence repeats from the invasive cotton mealybug phenacoccus solenopsis[J]. INSECT SCIENCE,2015,22(6):761-767.
APA	Li Hui,Lang KunLing,Fu HaiBin,Shen ChangPeng,Wan FangHao,&Chu Dong.(2015).Analysis of expressed sequence tags (ESTs) from a normalized cDNA library and isolation of EST simple sequence repeats from the invasive cotton mealybug phenacoccus solenopsis.INSECT SCIENCE,22(6),761-767.
MLA	Li Hui,et al."Analysis of expressed sequence tags (ESTs) from a normalized cDNA library and isolation of EST simple sequence repeats from the invasive cotton mealybug phenacoccus solenopsis".INSECT SCIENCE 22.6(2015):761-767.