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Tumor necrosis factor alpha increases intestinal permeability in mice with fulminant hepatic failure
Alternative TitleTumor necrosis factor alpha increases intestinal permeability in mice with fulminant hepatic failure
Li GuoZhen; Wang ZhaoHan; Cui Wei; Fu JinLong; Wang YuRong; Liu Pei
2012
Source PublicationWORLD JOURNAL OF GASTROENTEROLOGY
ISSN1007-9327
Volume18Issue:36Pages:5042-5050
AbstractAIM: To determine the effect of tumor necrosis factor alpha (TNF-alpha) on intestinal permeability (IP) in mice with fulminant hepatic failure (FHF), and the expression of tight junction proteins.
Other AbstractAIM:To determine the effect of tumor necrosis factor alpha(TNF-α) on intestinal permeability(IP) in mice with fulminant hepatic failure(FHF),and the expression of tight junction proteins.METHODS:We selected D-lactate as an index of IP,induced FHF using D-galactosamine/lipopolysaccharide and D-galactosamine/TNF-α,assessed the results using an enzymatic-spectrophotometric method,transmission electron microscopy,immunohistochemistry,Western blotting and real-time quantitative polymerase chain reaction.The effect of the administration of antiTNF-α immunoglobulin G(IgG) antibody,before the administration of D-galactosamine/lipopolysaccharide,on TNF-α was also assessed.RESULTS:IP was significantly increased in the mouse model of FHF 6 h after injection(13.57 ± 1.70 mg/L,13.02 ± 1.97 mg/L vs 3.76 ± 0.67 mg/L,P = 0.001).Electron microscopic analysis revealed tight junction(TJ) disruptions,epithelial cell swelling,and atrophy of intestinal villi.Expression of occludin and claudin-1 mRNA was significantly decreased in both FHF models(occludin:0.57 ± 0.159 fold vs baseline,P = 0.000;claudin-1:0.3067 ± 0.1291 fold vs baseline,P = 0.003),as were the distribution density of proteins in the intestinal mucosa and the levels of occludin and claudin-1 protein(occludin:0.61 ± 0.0473 fold vs baseline,P = 0.000;claudin-1:0.6633 ± 0.0328 fold vs baseline,P = 0.000).Prophylactic treatment with antiTNF-α IgG antibody prevented changes in IP(4.50 ± 0.97 mg/L vs 3.76 ± 0.67 mg/L,P = 0.791),intestinal tissue ultrastructure,and the mRNA levels of occludin and claudin-1 expression(occludin:0.8865 ± 0.0274 fold vs baseline,P = 0.505;claudin-1:0.85 ± 0.1437 fold vs baseline,P = 0.1),and in the protein levels(occludin:0.9467 ± 0.0285 fold vs baseline,P > 0.05;claudin-1:0.9533 ± 0.0186 fold vs baseline,P = 0.148).CONCLUSION:Increased in IP stemmed from the downregulation of the TJ proteins occludin and claudin-1,and destruction of the TJ in the colon,which were induced by TNF-α in FHF mice.
KeywordTIGHT JUNCTION PERMEABILITY ALCOHOLIC LIVER-DISEASE ENDOTOXIN OCCLUDIN RAT ACTIVATION PROTEIN PLASMA LIPOPOLYSACCHARIDES D(-)-LACTATE Tumor necrosis factor alpha Fulminant hepatic failure Intestinal permeability Occludin Claudin-1
Indexed ByCSCD
Language英语
Funding Project[National Ministry of Health of China]
CSCD IDCSCD:4653799
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Document Type期刊论文
Identifierhttp://ir.imr.ac.cn/handle/321006/147912
Collection中国科学院金属研究所
Affiliation中国科学院金属研究所
Recommended Citation
GB/T 7714
Li GuoZhen,Wang ZhaoHan,Cui Wei,et al. Tumor necrosis factor alpha increases intestinal permeability in mice with fulminant hepatic failure[J]. WORLD JOURNAL OF GASTROENTEROLOGY,2012,18(36):5042-5050.
APA Li GuoZhen,Wang ZhaoHan,Cui Wei,Fu JinLong,Wang YuRong,&Liu Pei.(2012).Tumor necrosis factor alpha increases intestinal permeability in mice with fulminant hepatic failure.WORLD JOURNAL OF GASTROENTEROLOGY,18(36),5042-5050.
MLA Li GuoZhen,et al."Tumor necrosis factor alpha increases intestinal permeability in mice with fulminant hepatic failure".WORLD JOURNAL OF GASTROENTEROLOGY 18.36(2012):5042-5050.
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