Expression of pituitary homeobox 1 gene in human gastric carcinogenesis and its clinicopathological significance

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	Expression of pituitary homeobox 1 gene in human gastric carcinogenesis and its clinicopathological significance
其他题名	Expression of pituitary homeobox 1 gene in human gastric carcinogenesis and its clinicopathological significance
	Chen YaNan; Chen Hong; Xu Yan; Zhang Xue; Luo Yang
	2008
发表期刊	WORLD JOURNAL OF GASTROENTEROLOGY
ISSN	1007-9327
卷号	14 期号:2 页码:292-297
摘要	AIM: To investigate the effect of pituitary homeobox 1 (PITX1) expression in cases of human gastric cancer on cancer differentiation and progression, and carcinogenesis.
其他摘要	AIM: To investigate the effect of pituitary homeobox 1 (PITX1) expression in cases of human gastric cancer on cancer differentiation and progression, and carcinogenesis.METHODS: Using polyclonal PITX1 antibodies, we studied the expression of PITX1 in normal gastric mucosa, atypical hyperplasia, intestinal metaplasia, and cancer tissue samples from 83 gastric cancer patients by immunohistochemistry. Moreover, semi-reverse transcription polymerase chain reaction (semi-RT-PCR)was performed to detect the mRNA level of PITX1 in three gastric cancer cell lines and a normal gastric epithelial cell line. Subsequently, somatic mutations of the PITX1 gene in 71 gastric cancer patients were analyzed by a combination of denaturing high performance liquid chromatography (DHPLC) and DNA sequencing.RESULTS: Immunohistochemistry showed that PITX1 was strongly or moderately expressed in the parietal cells of normal gastric mucosa (100%), while 55 (66.3%)out of 83 samples of gastric cancers showed decreased PITX1 expression.
关键词	HOMEOBOX GENE CANCER PITX1 TRANSCRIPTION PTX1 SUPPRESSOR REGULATOR CARCINOMA HINDLIMB TUMORS pituitary homeobox 1 gastric cancer immunohistochemistry reverse transcription polymerase chain reaction denaturing high performance liquid chromatography
收录类别	CSCD
语种	英语
CSCD记录号	CSCD:3317090
引用统计
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/148021
专题	中国科学院金属研究所
作者单位	中国科学院金属研究所
推荐引用方式 GB/T 7714	Chen YaNan,Chen Hong,Xu Yan,et al. Expression of pituitary homeobox 1 gene in human gastric carcinogenesis and its clinicopathological significance[J]. WORLD JOURNAL OF GASTROENTEROLOGY,2008,14(2):292-297.
APA	Chen YaNan,Chen Hong,Xu Yan,Zhang Xue,&Luo Yang.(2008).Expression of pituitary homeobox 1 gene in human gastric carcinogenesis and its clinicopathological significance.WORLD JOURNAL OF GASTROENTEROLOGY,14(2),292-297.
MLA	Chen YaNan,et al."Expression of pituitary homeobox 1 gene in human gastric carcinogenesis and its clinicopathological significance".WORLD JOURNAL OF GASTROENTEROLOGY 14.2(2008):292-297.