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Expression of pituitary homeobox 1 gene in human gastric carcinogenesis and its clinicopathological significance
Alternative TitleExpression of pituitary homeobox 1 gene in human gastric carcinogenesis and its clinicopathological significance
Chen YaNan; Chen Hong; Xu Yan; Zhang Xue; Luo Yang
2008
Source PublicationWORLD JOURNAL OF GASTROENTEROLOGY
ISSN1007-9327
Volume14Issue:2Pages:292-297
AbstractAIM: To investigate the effect of pituitary homeobox 1 (PITX1) expression in cases of human gastric cancer on cancer differentiation and progression, and carcinogenesis.
Other AbstractAIM: To investigate the effect of pituitary homeobox 1 (PITX1) expression in cases of human gastric cancer on cancer differentiation and progression, and carcinogenesis.METHODS: Using polyclonal PITX1 antibodies, we studied the expression of PITX1 in normal gastric mucosa, atypical hyperplasia, intestinal metaplasia, and cancer tissue samples from 83 gastric cancer patients by immunohistochemistry. Moreover, semi-reverse transcription polymerase chain reaction (semi-RT-PCR)was performed to detect the mRNA level of PITX1 in three gastric cancer cell lines and a normal gastric epithelial cell line. Subsequently, somatic mutations of the PITX1 gene in 71 gastric cancer patients were analyzed by a combination of denaturing high performance liquid chromatography (DHPLC) and DNA sequencing.RESULTS: Immunohistochemistry showed that PITX1 was strongly or moderately expressed in the parietal cells of normal gastric mucosa (100%), while 55 (66.3%)out of 83 samples of gastric cancers showed decreased PITX1 expression.
KeywordHOMEOBOX GENE CANCER PITX1 TRANSCRIPTION PTX1 SUPPRESSOR REGULATOR CARCINOMA HINDLIMB TUMORS pituitary homeobox 1 gastric cancer immunohistochemistry reverse transcription polymerase chain reaction denaturing high performance liquid chromatography
Indexed ByCSCD
Language英语
CSCD IDCSCD:3317090
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Document Type期刊论文
Identifierhttp://ir.imr.ac.cn/handle/321006/148021
Collection中国科学院金属研究所
Affiliation中国科学院金属研究所
Recommended Citation
GB/T 7714
Chen YaNan,Chen Hong,Xu Yan,et al. Expression of pituitary homeobox 1 gene in human gastric carcinogenesis and its clinicopathological significance[J]. WORLD JOURNAL OF GASTROENTEROLOGY,2008,14(2):292-297.
APA Chen YaNan,Chen Hong,Xu Yan,Zhang Xue,&Luo Yang.(2008).Expression of pituitary homeobox 1 gene in human gastric carcinogenesis and its clinicopathological significance.WORLD JOURNAL OF GASTROENTEROLOGY,14(2),292-297.
MLA Chen YaNan,et al."Expression of pituitary homeobox 1 gene in human gastric carcinogenesis and its clinicopathological significance".WORLD JOURNAL OF GASTROENTEROLOGY 14.2(2008):292-297.
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