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KAI1 is a potential target for anti-metastasis in pancreatic cancer cells
Alternative TitleKAI1 is a potential target for anti-metastasis in pancreatic cancer cells
Xu JianHua; Guo XiaoZhong; Ren LiNan; Shao LiChun; Liu MinPei
2008
Source PublicationWORLD JOURNAL OF GASTROENTEROLOGY
ISSN1007-9327
Volume14Issue:7Pages:1126-1132
AbstractAIM: To investigate whether KAI1, as a metastasis suppressor gene, is associated with invasive and metastatic ability of pancreatic cancer cells.
Other AbstractAIM:To investigate whether KAI1,as a metastasis suppressor gene,is associated with invasive and metastatic ability of pancreatic cancer cells.METHODS:KAI1 gene was transfected into pancreatic cancer cell line MiaPaCa Ⅱ by liposomes selected with G418.Expression of transfected cells was measured by Western blotting,immunofluorescence and immunocytochemistry.Tumor cell invasion and metastatic ability were detected through gelatinase activity and reconstituted basement membrane (Matrigel) assay,pCMV-KAI1 was directly injected into the heterotopic human pancreatic adenocarcinoma successfully established in the groin of BALB/C nude mice,by subcutaneous injection of MiaPaCa Ⅱ pancreatic cancer cells.The statistical analysis between groups was determined by Student's two tailed t test.RESULTS:By Western blotting,NiaPaCa Ⅱ cells transfected by KAI1 gene indicated KAI1 expression at approximately 29.1 kDa.Cytoplasm staining was positive and uniformly spread in transfected cancer cells,using immunohistochemistry and immunofluorescence.The most obvious difference was present after 30 h (MiaPaca Ⅱ43.6±9.42,pCMV-MiaPaca Ⅱ 44.8±8.56,pCMVKAI1-MiaPaca Ⅱ 22.0±4.69,P<0.05).Gelatinolysisrevealed a wider and clearer band of gelatinolytic activity in non-transfected than in transfected cells (MiaPaCa Ⅱ cells 30.8±0.57,transfected cells 28.1±0.65,P<0.05).In vivo tumor growth rates of KAI1 transfectants with KAI1-Lipofectamine 1.22±0.31 in A group were lower than control 4.61±1.98 and pCMV-KAI 11.67±0.81.Analyses of metastases with and without KAI1 transfection in mice were different in liver and lung between controls 1.62±0.39,0.45±0.09,pCMVKAI 1.01±0.27,0.33±0.09 and KAI1-Lipofectamine 0.99±0.21,0.30±0.09 respectively (P<0.05).CONCLUSION:High expression of KAI1 gene was found in transfected MiaPaCa Ⅱ human pancreatic cancer cells with lower metastatic ability.KAI1 gene plays an important role in inhibiting metastasis of pancreatic cancer after direct injection into pancreatic adenocarcinoma.These results show that the suppressed invasion and motor function of pancreatic cancer cells may be a key reason why the KAI1 gene controls pancreatic cancer cell metastasis.
KeywordMATRIX METALLOPROTEINASES SUPPRESSOR GENE DUCTAL ADENOCARCINOMA PROSTATE-CANCER TUMOR INVASION EXPRESSION PROGNOSIS P53 PROGRESSION INHIBITORS KAI1 pancreatic cancer cell line transfection immunocytochemistry western blotting immunofluorescence gelatinolysis
Indexed ByCSCD
Language英语
CSCD IDCSCD:3317226
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Document Type期刊论文
Identifierhttp://ir.imr.ac.cn/handle/321006/148320
Collection中国科学院金属研究所
Affiliation中国科学院金属研究所
Recommended Citation
GB/T 7714
Xu JianHua,Guo XiaoZhong,Ren LiNan,et al. KAI1 is a potential target for anti-metastasis in pancreatic cancer cells[J]. WORLD JOURNAL OF GASTROENTEROLOGY,2008,14(7):1126-1132.
APA Xu JianHua,Guo XiaoZhong,Ren LiNan,Shao LiChun,&Liu MinPei.(2008).KAI1 is a potential target for anti-metastasis in pancreatic cancer cells.WORLD JOURNAL OF GASTROENTEROLOGY,14(7),1126-1132.
MLA Xu JianHua,et al."KAI1 is a potential target for anti-metastasis in pancreatic cancer cells".WORLD JOURNAL OF GASTROENTEROLOGY 14.7(2008):1126-1132.
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