Effects of transferred NK4 gene on proliferation, migration, invasion and apoptosis of human prostate cancer DU145 cells

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	Effects of transferred NK4 gene on proliferation, migration, invasion and apoptosis of human prostate cancer DU145 cells
其他题名	Effects of transferred NK4 gene on proliferation, migration, invasion and apoptosis of human prostate cancer DU145 cells
	Yue Dan; Wang Yong; Ma Ping; Li YinYan; Chen Hong; Wang Ping; Ren ChangShan
	2010
发表期刊	ASIAN JOURNAL OF ANDROLOGY
ISSN	1008-682X
卷号	12 期号:3 页码:381-389
摘要	We investigated the ability of NK4, an antagonist of human hepatocyte growth factor (HGF), to inhibit the influence of HGF on proliferation, migration, invasion and apoptosis of human prostate cancer cells. Expression vector pBudCE4.1-EGFP-NK4 containing NK4 cDNA was used to transfect human prostate cancer DU145 cells, and the effects of the autocrine NK4 on tumor cell proliferation, migration, invasion and apoptosis were assessed in vitro. In vivo, we subcutaneously implanted DU145 cells, mock-transfected clone (DU145/empty vector) cells and NK4-transfected clone (DU145/NK4) cells into nude mice, and then evaluated tumor growth, cell proliferation and cell apoptosis in vivo. We found that DU145/NK4 cells expressed NK4 protein. In the in vitro study, autocrine NK4 attenuated the HGF-induced tumor cell proliferation, migration and invasion, and stimulated apoptosis. Furthermore, autocrine NK4 effectively inhibited the HGF-induced phosphorylation of c-Met, extracellular signal-regulated kinase-1 (ERK1). and protein kinase B 1/2 (Akt1/2). Histological examination revealed that autocrine NK4 inhibited proliferation and accelerated apoptosis of prostate cancer cells. These results show that genetic modification of DU145 cells with NK4 cDNA yields a significant effect on their proliferation, migration, invasion and apoptosis. Molecular targeting of HGF/c-Met by NK4 could be applied as a novel therapeutic approach to prostate cancer.
其他摘要	We investigated the ability of NK4, an antagonist of human hepatocyte growth factor (HGF), to inhibit the influence of HGF on proliferation, migration, invasion and apoptosis of human prostate cancer cells. Expression vector pBudCE4.1-EGFP-NK4 containing NK4 cDNA was used to transfect human prostate cancer DU145 cells, and the effects of the autocrine NK4 on tumor cell proliferation, migration, invasion and apoptosis were assessed in vitro . In vivo , we subcutaneously implanted DU145 cells, mock-transfected clone (DU145 in vivo . We found that DU145 in vitro study, autocrine NK4 attenuated the HGF-induced tumor cell proliferation, migration and invasion, and stimulated apoptosis. Furthermore, autocrine NK4 effectively inhibited the HGF-induced phosphorylation of c-Met, extracellular signal-regulated kinase-1 (ERK1). and protein kinase B 1
关键词	HEPATOCYTE GROWTH-FACTOR RECEPTOR C-MET NUDE-MICE HGF ANGIOGENESIS METASTASIS ANTAGONIST BENIGN EXPRESSION CARCINOMA hepatocyte growth factor human prostate cancer NK4 DU145 cells
收录类别	CSCD
语种	英语
CSCD记录号	CSCD:3891332
引用统计	被引频次：6[CSCD] [CSCD记录]
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/148566
专题	中国科学院金属研究所
作者单位	中国科学院金属研究所
推荐引用方式 GB/T 7714	Yue Dan,Wang Yong,Ma Ping,et al. Effects of transferred NK4 gene on proliferation, migration, invasion and apoptosis of human prostate cancer DU145 cells[J]. ASIAN JOURNAL OF ANDROLOGY,2010,12(3):381-389.
APA	Yue Dan.,Wang Yong.,Ma Ping.,Li YinYan.,Chen Hong.,...&Ren ChangShan.(2010).Effects of transferred NK4 gene on proliferation, migration, invasion and apoptosis of human prostate cancer DU145 cells.ASIAN JOURNAL OF ANDROLOGY,12(3),381-389.
MLA	Yue Dan,et al."Effects of transferred NK4 gene on proliferation, migration, invasion and apoptosis of human prostate cancer DU145 cells".ASIAN JOURNAL OF ANDROLOGY 12.3(2010):381-389.