| Mass Spectrometery Assay for Denitrifying Enzyme Activity Measurement | |
| Alternative Title | Mass spectrometery assay for denitrifying enzyme activity measurement |
| Zhang Lili1; Wu Zhijie1; Song Yuchao2 | |
| 2010 | |
| Source Publication | SPECTROSCOPY AND SPECTRAL ANALYSIS
 |
| ISSN | 1000-0593 |
| Volume | 30Issue:7Pages:2011-2013 |
| Abstract | Nitrification inhibitors exert inhibition function in soil nitrification process (NF4+-N to NO3--N) and are widely applied in order to improve N fertilizer use efficiency. Before the new nitrification inhibitor is used, its effects on denitrification process must be investigated and denitrifying enzyme activity (DEA) is an effective indicator to show this process. In the present paper, a mass spectrometery(MS) method was taken to measure the denitrifying enzyme activity in the new nitrification inhibitor 3, 4-dimethylpyrazole phosphate(DMPP) incubation system. The results showed that the method measured the concentration of N2O in the incubation system accurately and the concentration measured by MS had marked correlation with that measured by gas chromatogram(GC) (MSN2O = -0.45 + 1.03GC(N2O), R-2 = 0.995). At the same time, enrichment of (N2O)-N-15 and N-15(2) was measured to discriminate the nitrate reductase and N2O reductase. Differed with traditional method, the enzymatic inhibitor-acetylene was not appended. The results showed that DMPP had no influence on the denitrifying enzyme activity and indicated that the denitrification process was not influenced by DMPP. |
| Other Abstract | Nitrification inhibitors exert inhibition function in soil nitrification process (nf(4)(+)-n to no(3)(-)-n) and are widely applied in order to improve n fertilizer use efficiency. before the new nitrification inhibitor is used, its effects on denitrification process must be investigated and denitrifying enzyme activity (dea) is an effective indicator to show this process. in the present paper, a mass spectrometery(ms) method was taken to measure the denitrifying enzyme activity in the new nitrification inhibitor 3, 4-dimethylpyrazole phosphate(dmpp) incubation system. the results showed that the method measured the concentration of n(2)o in the incubation system accurately and the concentration measured by ms had marked correlation with that measured by gas chromatogram(gc) (ms(n2o) = -0.45 + 1.03gc(n2o), r(2) = 0.995). at the same time, enrichment of (15)n(2)o and (15)n(2) was measured to discriminate the nitrate reductase and n(2)o reductase. differed with traditional method, the enzymatic inhibitor-acetylene was not appended. the results showed that dmpp had no influence on the denitrifying enzyme activity and indicated that the denitrification process was not influenced by dmpp. |
| Keyword | NITROUS-OXIDE NITRIFICATION DINITROGEN SOILS N-15 Mass spectrometery Denitrifying enzyme activity 3,4-dimethylpyrazole phosphate |
| Indexed By | CSCD |
| Language | 英语 |
| CSCD ID | CSCD:3913888 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.imr.ac.cn/handle/321006/148902 |
| Collection | 中国科学院金属研究所 |
| Affiliation | 1.中国科学院 2.中国科学院金属研究所 |
| Recommended Citation GB/T 7714 | Zhang Lili,Wu Zhijie,Song Yuchao. Mass Spectrometery Assay for Denitrifying Enzyme Activity Measurement[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS,2010,30(7):2011-2013. |
| APA | Zhang Lili,Wu Zhijie,&Song Yuchao.(2010).Mass Spectrometery Assay for Denitrifying Enzyme Activity Measurement.SPECTROSCOPY AND SPECTRAL ANALYSIS,30(7),2011-2013. |
| MLA | Zhang Lili,et al."Mass Spectrometery Assay for Denitrifying Enzyme Activity Measurement".SPECTROSCOPY AND SPECTRAL ANALYSIS 30.7(2010):2011-2013. |
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