Mass Spectrometery Assay for Denitrifying Enzyme Activity Measurement

IMR OpenIR

	Mass Spectrometery Assay for Denitrifying Enzyme Activity Measurement
其他题名	Mass spectrometery assay for denitrifying enzyme activity measurement
	Zhang Lili 1; Wu Zhijie 1; Song Yuchao 2
	2010
发表期刊	SPECTROSCOPY AND SPECTRAL ANALYSIS
ISSN	1000-0593
卷号	30 期号:7 页码:2011-2013
摘要	Nitrification inhibitors exert inhibition function in soil nitrification process (NF4+-N to NO3--N) and are widely applied in order to improve N fertilizer use efficiency. Before the new nitrification inhibitor is used, its effects on denitrification process must be investigated and denitrifying enzyme activity (DEA) is an effective indicator to show this process. In the present paper, a mass spectrometery(MS) method was taken to measure the denitrifying enzyme activity in the new nitrification inhibitor 3, 4-dimethylpyrazole phosphate(DMPP) incubation system. The results showed that the method measured the concentration of N2O in the incubation system accurately and the concentration measured by MS had marked correlation with that measured by gas chromatogram(GC) (MSN2O = -0.45 + 1.03GC(N2O), R-2 = 0.995). At the same time, enrichment of (N2O)-N-15 and N-15(2) was measured to discriminate the nitrate reductase and N2O reductase. Differed with traditional method, the enzymatic inhibitor-acetylene was not appended. The results showed that DMPP had no influence on the denitrifying enzyme activity and indicated that the denitrification process was not influenced by DMPP.
其他摘要	Nitrification inhibitors exert inhibition function in soil nitrification process (nf(4)(+)-n to no(3)(-)-n) and are widely applied in order to improve n fertilizer use efficiency. before the new nitrification inhibitor is used, its effects on denitrification process must be investigated and denitrifying enzyme activity (dea) is an effective indicator to show this process. in the present paper, a mass spectrometery(ms) method was taken to measure the denitrifying enzyme activity in the new nitrification inhibitor 3, 4-dimethylpyrazole phosphate(dmpp) incubation system. the results showed that the method measured the concentration of n(2)o in the incubation system accurately and the concentration measured by ms had marked correlation with that measured by gas chromatogram(gc) (ms(n2o) = -0.45 + 1.03gc(n2o), r(2) = 0.995). at the same time, enrichment of (15)n(2)o and (15)n(2) was measured to discriminate the nitrate reductase and n(2)o reductase. differed with traditional method, the enzymatic inhibitor-acetylene was not appended. the results showed that dmpp had no influence on the denitrifying enzyme activity and indicated that the denitrification process was not influenced by dmpp.
关键词	NITROUS-OXIDE NITRIFICATION DINITROGEN SOILS N-15 Mass spectrometery Denitrifying enzyme activity 3,4-dimethylpyrazole phosphate
收录类别	CSCD
语种	英语
CSCD记录号	CSCD:3913888
引用统计
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/148902
专题	中国科学院金属研究所
作者单位	1.中国科学院 2.中国科学院金属研究所
推荐引用方式 GB/T 7714	Zhang Lili,Wu Zhijie,Song Yuchao. Mass Spectrometery Assay for Denitrifying Enzyme Activity Measurement[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS,2010,30(7):2011-2013.
APA	Zhang Lili,Wu Zhijie,&Song Yuchao.(2010).Mass Spectrometery Assay for Denitrifying Enzyme Activity Measurement.SPECTROSCOPY AND SPECTRAL ANALYSIS,30(7),2011-2013.
MLA	Zhang Lili,et al."Mass Spectrometery Assay for Denitrifying Enzyme Activity Measurement".SPECTROSCOPY AND SPECTRAL ANALYSIS 30.7(2010):2011-2013.