|Alteration of SlYABBY2b gene expression impairs tomato ovary locule number and endogenous gibberellin content|
|Li Hui; Sun Meihua; Qi Mingfang; Xing Jiao; Xu Tao; Liu Hanting; Li Tianlai
|Source Publication||JOURNAL OF ZHEJIANG UNIVERSITY-SCIENCE B
|Abstract||Tomato is an ideal model species for fleshy fruit development research. SlYABBY2b regulates the ovary locule number, which is increased by gibberellins, in tomato. However, the relationship between SlYABBY2b and endogenous gibberellin is poorly understood. In this study, SlYABBY2b-overexpressing and RNA interference (RNAi) transgenic tomato plants were used to elucidate the mechanism by which SlYABBY2b regulates the ovary locule number and endogenous gibberellin content in tomato. SlYABBY2b-overexpressing plants showed fewer locules and lower gibberellin content than the control plants. Contrasting results were found in the RNAi lines. Therefore, the SlYABBY2b gene negatively regulates tomato ovary locule number and endogenous gibberellin content. Furthermore, the expression of SlYABBY2b gene was remarkably higher than that of the wild type in the apical shoots of gibberellindeficient mutants. This showed that the gibberellins can inhibit the expression of SlYABBY2b gene negative regulation. Further study revealed that SlYABBY2b suppressed the expression of SlGA20ox1 and SlGA3ox2, but increased that of SlGA2ox1 and SlGA2ox5 in the apical shoots of SlYABBY2b-overexpressing plants, thereby reducing gibberellin content. Contrasting results were found in the RNAi lines. Our results showed that the SlYABBY2b gene was located on gibberellin signal transduction pathways, fed back regulation of the synthesis of gibberellin, and felt exogenous gibberellin signal to further regulate the formation of tomato locule.|
|Keyword||REPRESSION OFSHOOT GROWTH
EXTREME FRUIT SIZE
Ovary locule number
|Funding Project||[China Agriculture Research System]
; [Program for Liaoning Innovative Research Team in University]
; [Program for Liaoning Key Laboratory, China]
Li Hui,Sun Meihua,Qi Mingfang,et al. Alteration of SlYABBY2b gene expression impairs tomato ovary locule number and endogenous gibberellin content[J]. JOURNAL OF ZHEJIANG UNIVERSITY-SCIENCE B,2018,19(6):445-457.
Li Hui.,Sun Meihua.,Qi Mingfang.,Xing Jiao.,Xu Tao.,...&Li Tianlai.(2018).Alteration of SlYABBY2b gene expression impairs tomato ovary locule number and endogenous gibberellin content.JOURNAL OF ZHEJIANG UNIVERSITY-SCIENCE B,19(6),445-457.
Li Hui,et al."Alteration of SlYABBY2b gene expression impairs tomato ovary locule number and endogenous gibberellin content".JOURNAL OF ZHEJIANG UNIVERSITY-SCIENCE B 19.6(2018):445-457.
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