Alteration of SlYABBY2b gene expression impairs tomato ovary locule number and endogenous gibberellin content

IMR OpenIR

	Alteration of SlYABBY2b gene expression impairs tomato ovary locule number and endogenous gibberellin content
	Li Hui; Sun Meihua; Qi Mingfang; Xing Jiao; Xu Tao; Liu Hanting; Li Tianlai
	2018
发表期刊	JOURNAL OF ZHEJIANG UNIVERSITY-SCIENCE B
ISSN	1673-1581
卷号	19 期号:6 页码:445-457
摘要	Tomato is an ideal model species for fleshy fruit development research. SlYABBY2b regulates the ovary locule number, which is increased by gibberellins, in tomato. However, the relationship between SlYABBY2b and endogenous gibberellin is poorly understood. In this study, SlYABBY2b-overexpressing and RNA interference (RNAi) transgenic tomato plants were used to elucidate the mechanism by which SlYABBY2b regulates the ovary locule number and endogenous gibberellin content in tomato. SlYABBY2b-overexpressing plants showed fewer locules and lower gibberellin content than the control plants. Contrasting results were found in the RNAi lines. Therefore, the SlYABBY2b gene negatively regulates tomato ovary locule number and endogenous gibberellin content. Furthermore, the expression of SlYABBY2b gene was remarkably higher than that of the wild type in the apical shoots of gibberellindeficient mutants. This showed that the gibberellins can inhibit the expression of SlYABBY2b gene negative regulation. Further study revealed that SlYABBY2b suppressed the expression of SlGA20ox1 and SlGA3ox2, but increased that of SlGA2ox1 and SlGA2ox5 in the apical shoots of SlYABBY2b-overexpressing plants, thereby reducing gibberellin content. Contrasting results were found in the RNAi lines. Our results showed that the SlYABBY2b gene was located on gibberellin signal transduction pathways, fed back regulation of the synthesis of gibberellin, and felt exogenous gibberellin signal to further regulate the formation of tomato locule.
关键词	REPRESSION OFSHOOT GROWTH EXTREME FRUIT SIZE TRANSCRIPTIONAL ACTIVATOR MERISTEM SIZE ARABIDOPSIS BIOSYNTHESIS METABOLISM OVEREXPRESSION IDENTIFICATION MALFORMATION SlYABBY2b Gibberellin Gibberellin-deficient mutants Ovary locule number Transgenic plant Gene expression Tomato
收录类别	CSCD
语种	英语
资助项目	[China Agriculture Research System] ; [Program for Liaoning Innovative Research Team in University] ; [Program for Liaoning Key Laboratory, China]
CSCD记录号	CSCD:6272023
引用统计
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/148931
专题	中国科学院金属研究所
作者单位	中国科学院金属研究所
推荐引用方式 GB/T 7714	Li Hui,Sun Meihua,Qi Mingfang,et al. Alteration of SlYABBY2b gene expression impairs tomato ovary locule number and endogenous gibberellin content[J]. JOURNAL OF ZHEJIANG UNIVERSITY-SCIENCE B,2018,19(6):445-457.
APA	Li Hui.,Sun Meihua.,Qi Mingfang.,Xing Jiao.,Xu Tao.,...&Li Tianlai.(2018).Alteration of SlYABBY2b gene expression impairs tomato ovary locule number and endogenous gibberellin content.JOURNAL OF ZHEJIANG UNIVERSITY-SCIENCE B,19(6),445-457.
MLA	Li Hui,et al."Alteration of SlYABBY2b gene expression impairs tomato ovary locule number and endogenous gibberellin content".JOURNAL OF ZHEJIANG UNIVERSITY-SCIENCE B 19.6(2018):445-457.