骨生长因子及骨钙素在新西兰大耳兔体内植入早期镁合金/新骨生成界面的表达变化

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	骨生长因子及骨钙素在新西兰大耳兔体内植入早期镁合金/新骨生成界面的表达变化
其他题名	In vivo expression at early stage of bone growth factors and osteocalcin on the magnesium alloy/bone interface in New Zealand white rabbits
	潘锋 1; 柏树令 1; 张二林 2; 徐丽萍 2; 于国宁 3
	2010
发表期刊	解剖学报
ISSN	0529-1356
卷号	000 期号:004 页码:576-580
摘要	目的观察3种骨生长因子及骨钙素在动物体内植入早期镁合金/新骨生成界面的表达变化. 方法将具有钙磷涂层的Mg-Mn-Zn合金和对照组裸Mg-Mn-Zn合金分别植入新西兰大耳白兔股骨的上、下干骺端,皮下注射钙黄绿素.在植入1、2、3、4周后分别做X线检查、HE染色、荧光观察和骨形态发生蛋白（BMP）、转化生长因子-β1（TGF-β1）、血小板衍生生长因子（PDGF）、骨钙素（BGP）的免疫组织化学染色. 结果 X线检查见植入4周后的实验组与对照组的合金棒均被新生骨质包裹 HE染色及荧光染色未见实验组与对照组之间有明显差异免疫组织化学染色发现,实验组与对照组之间的BMP-2、TGF-β1和PDGF的表达存在明显差异,实验组的表达明显高于对照组. 结论具有钙磷涂层的Mg-Mn-Zn合金比对照组合金更好地促进骨髓腔内移植物界面处新骨生长因子的表达.
其他摘要	Objective To investigate in vivo expression at early stage of three bone growth factors and osteoealcin on magnesium alloy/bone interface in New Zealand white rabbits. Methods The Ca-P coated and the naked Mg-Mn-Zn alloy rods were implanted respectivelys into the left femoral metaphysis of rabbits. Intravital staining was performed using subcutaneous injections of 1% water solution of calcein to observe the newly formed bone. Postoperatively, three rabbits were sacrificed at 1 , 2, 3 and 4 weeks for X-ray examination, fluorescent observation, routine pathological examination and bone morphogenetic protein （BMP） , transforming growth factor β1 （ TGF-β1 ） , platelet-derived growth factor （PDGF） and bone gla protein（BGP） immunohistochemistry. Results X-ray examination showed that both experimental and controlled rods were encircled by new bone, and HE staining and fluorescent observation showed no differences between two groups, but immunohistoehemistry and associated statistical ananlysis displayed obvious differences on average absorbance values of BMP-2, TGF-β1 and PDGF expression. Average absorbance values of experimental group expressed higher than thoese of controlled group. Conclusion Ca-P coated Mg-Mn-Zn promotes the expression of bone growth factors than that of naked Mg-Mn-Zn at the implant/bone interface in marrow of rabbits.
关键词	钙磷涂层Mg-Mn-Zn 骨生长因子骨钙素免疫组织化学兔
收录类别	CSCD
语种	中文
CSCD记录号	CSCD:3966394
引用统计
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/148946
专题	中国科学院金属研究所
作者单位	1.中国医科大学 2.中国科学院金属研究所 3.辽宁省人民医院
推荐引用方式 GB/T 7714	潘锋,柏树令,张二林,等. 骨生长因子及骨钙素在新西兰大耳兔体内植入早期镁合金/新骨生成界面的表达变化[J]. 解剖学报,2010,000(004):576-580.
APA	潘锋,柏树令,张二林,徐丽萍,&于国宁.(2010).骨生长因子及骨钙素在新西兰大耳兔体内植入早期镁合金/新骨生成界面的表达变化.解剖学报,000(004),576-580.
MLA	潘锋,et al."骨生长因子及骨钙素在新西兰大耳兔体内植入早期镁合金/新骨生成界面的表达变化".解剖学报 000.004(2010):576-580.