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A rapid approach for isolating a single fungal spore from rice blast diseased leaves
Alternative TitleA rapid approach for isolating a single fungal spore from rice blast diseased leaves
Fei Liwang1; Lu Wenbo1; Xu Xiaozhou1; Yan Fengcheng1; Zhang Liwei1; Lw Jintao1; Bai Yuanjun2; Li Zhenyu3; Zhao Wensheng1; Yang Jun1; Peng Youliang1
2019
Source PublicationJOURNAL OF INTEGRATIVE AGRICULTURE
ISSN2095-3119
Volume18Issue:6Pages:1415-1418
AbstractSingle spore isolation is a fundamental approach in plant pathology and mycology to isolate and identify plant fungal pathogens from diseased samples. However, routine single spore isolation procedure is time-consuming and has a high risk of contamination by other microorganisms. In this study, we developed a rapid approach for isolating a single spore of the fungal pathogen, Pyricularia oryzae, from rice blast diseased leaves in the paddy field with low potential of contamination. First, rice blast leaves with single lesions were selected in the paddy field, and a single lesion was cut out and pressed and dragged gently across the surface of water agar. Next, a germinated single spore with a barely visible piece of agar was cut out of water agar with a dissecting needle under a stereomicroscope at approximately 120-fold magnification. Last, the germinated single spore with water agar was transferred onto oatmeal tomato agar for growth and preservation. Based on our experience, a skilled technician or student can successfully isolate single spore from over 150 independent diseased samples with nearly no contaminations in a single working day. This approach is also suitable for isolating single spore from other fungal disease samples that produce abundant aerial conidia.
Other AbstractSingle spore isolation is a fundamental approach in plant pathology and mycology to isolate and identify plant fungal pathogens from diseased samples. However, routine single spore isolation procedure is time-consuming and has a high risk of contamination by other microorganisms. In this study, we developed a rapid approach for isolating a single spore of the fungal pathogen, Pyricularia oryzae, from rice blast diseased leaves in the paddy field with low potential of contamination. First, rice blast leaves with single lesions were selected in the paddy field, and a single lesion was cut out and pressed and dragged gently across the surface of water agar. Next, a germinated single spore with a barely visible piece of agar was cut out of water agar with a dissecting needle under a stereomicroscope at approximately 120-fold magnification. Last, the germinated single spore with water agar was transferred onto oatmeal tomato agar for growth and preservation. Based on our experience, a skilled technician or student can successfully isolate single spore from over 150 independent diseased samples with nearly no contaminations in a single working day. This approach is also suitable for isolating single spore from other fungal disease samples that produce abundant aerial conidia.
KeywordPyricularia oryzae single spore isolation rice blast field experiment
Indexed ByCSCD
Language英语
Funding Project[earmarked fund for China Agricultural Research System] ; [National Key Research and Development Program of China]
CSCD IDCSCD:6517127
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Document Type期刊论文
Identifierhttp://ir.imr.ac.cn/handle/321006/149259
Collection中国科学院金属研究所
Affiliation1.中国科学院亚热带农业生态研究所
2.中国科学院金属研究所
3.Liaoning Acad Agr Sci, Saline Alkali Land Utilizat & Res Institute, Panjin 124010, Peoples R China
Recommended Citation
GB/T 7714
Fei Liwang,Lu Wenbo,Xu Xiaozhou,et al. A rapid approach for isolating a single fungal spore from rice blast diseased leaves[J]. JOURNAL OF INTEGRATIVE AGRICULTURE,2019,18(6):1415-1418.
APA Fei Liwang.,Lu Wenbo.,Xu Xiaozhou.,Yan Fengcheng.,Zhang Liwei.,...&Peng Youliang.(2019).A rapid approach for isolating a single fungal spore from rice blast diseased leaves.JOURNAL OF INTEGRATIVE AGRICULTURE,18(6),1415-1418.
MLA Fei Liwang,et al."A rapid approach for isolating a single fungal spore from rice blast diseased leaves".JOURNAL OF INTEGRATIVE AGRICULTURE 18.6(2019):1415-1418.
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