| A rapid approach for isolating a single fungal spore from rice blast diseased leaves | |
| Alternative Title | A rapid approach for isolating a single fungal spore from rice blast diseased leaves |
| Fei Liwang1; Lu Wenbo1; Xu Xiaozhou1; Yan Fengcheng1; Zhang Liwei1; Lw Jintao1; Bai Yuanjun2; Li Zhenyu3; Zhao Wensheng1; Yang Jun1; Peng Youliang1 | |
| 2019 | |
| Source Publication | JOURNAL OF INTEGRATIVE AGRICULTURE
 |
| ISSN | 2095-3119 |
| Volume | 18Issue:6Pages:1415-1418 |
| Abstract | Single spore isolation is a fundamental approach in plant pathology and mycology to isolate and identify plant fungal pathogens from diseased samples. However, routine single spore isolation procedure is time-consuming and has a high risk of contamination by other microorganisms. In this study, we developed a rapid approach for isolating a single spore of the fungal pathogen, Pyricularia oryzae, from rice blast diseased leaves in the paddy field with low potential of contamination. First, rice blast leaves with single lesions were selected in the paddy field, and a single lesion was cut out and pressed and dragged gently across the surface of water agar. Next, a germinated single spore with a barely visible piece of agar was cut out of water agar with a dissecting needle under a stereomicroscope at approximately 120-fold magnification. Last, the germinated single spore with water agar was transferred onto oatmeal tomato agar for growth and preservation. Based on our experience, a skilled technician or student can successfully isolate single spore from over 150 independent diseased samples with nearly no contaminations in a single working day. This approach is also suitable for isolating single spore from other fungal disease samples that produce abundant aerial conidia. |
| Other Abstract | Single spore isolation is a fundamental approach in plant pathology and mycology to isolate and identify plant fungal pathogens from diseased samples. However, routine single spore isolation procedure is time-consuming and has a high risk of contamination by other microorganisms. In this study, we developed a rapid approach for isolating a single spore of the fungal pathogen, Pyricularia oryzae, from rice blast diseased leaves in the paddy field with low potential of contamination. First, rice blast leaves with single lesions were selected in the paddy field, and a single lesion was cut out and pressed and dragged gently across the surface of water agar. Next, a germinated single spore with a barely visible piece of agar was cut out of water agar with a dissecting needle under a stereomicroscope at approximately 120-fold magnification. Last, the germinated single spore with water agar was transferred onto oatmeal tomato agar for growth and preservation. Based on our experience, a skilled technician or student can successfully isolate single spore from over 150 independent diseased samples with nearly no contaminations in a single working day. This approach is also suitable for isolating single spore from other fungal disease samples that produce abundant aerial conidia. |
| Keyword | Pyricularia oryzae single spore isolation rice blast field experiment |
| Indexed By | CSCD |
| Language | 英语 |
| Funding Project | [earmarked fund for China Agricultural Research System] ; [National Key Research and Development Program of China] |
| CSCD ID | CSCD:6517127 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.imr.ac.cn/handle/321006/149259 |
| Collection | 中国科学院金属研究所 |
| Affiliation | 1.中国科学院亚热带农业生态研究所 2.中国科学院金属研究所 3.Liaoning Acad Agr Sci, Saline Alkali Land Utilizat & Res Institute, Panjin 124010, Peoples R China |
| Recommended Citation GB/T 7714 | Fei Liwang,Lu Wenbo,Xu Xiaozhou,et al. A rapid approach for isolating a single fungal spore from rice blast diseased leaves[J]. JOURNAL OF INTEGRATIVE AGRICULTURE,2019,18(6):1415-1418. |
| APA | Fei Liwang.,Lu Wenbo.,Xu Xiaozhou.,Yan Fengcheng.,Zhang Liwei.,...&Peng Youliang.(2019).A rapid approach for isolating a single fungal spore from rice blast diseased leaves.JOURNAL OF INTEGRATIVE AGRICULTURE,18(6),1415-1418. |
| MLA | Fei Liwang,et al."A rapid approach for isolating a single fungal spore from rice blast diseased leaves".JOURNAL OF INTEGRATIVE AGRICULTURE 18.6(2019):1415-1418. |
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