A rapid approach for isolating a single fungal spore from rice blast diseased leaves

IMR OpenIR

	A rapid approach for isolating a single fungal spore from rice blast diseased leaves
其他题名	A rapid approach for isolating a single fungal spore from rice blast diseased leaves
	Fei Liwang 1; Lu Wenbo 1; Xu Xiaozhou 1; Yan Fengcheng 1; Zhang Liwei 1; Lw Jintao 1; Bai Yuanjun 2; Li Zhenyu 3; Zhao Wensheng 1; Yang Jun 1; Peng Youliang 1
	2019
发表期刊	JOURNAL OF INTEGRATIVE AGRICULTURE
ISSN	2095-3119
卷号	18 期号:6 页码:1415-1418
摘要	Single spore isolation is a fundamental approach in plant pathology and mycology to isolate and identify plant fungal pathogens from diseased samples. However, routine single spore isolation procedure is time-consuming and has a high risk of contamination by other microorganisms. In this study, we developed a rapid approach for isolating a single spore of the fungal pathogen, Pyricularia oryzae, from rice blast diseased leaves in the paddy field with low potential of contamination. First, rice blast leaves with single lesions were selected in the paddy field, and a single lesion was cut out and pressed and dragged gently across the surface of water agar. Next, a germinated single spore with a barely visible piece of agar was cut out of water agar with a dissecting needle under a stereomicroscope at approximately 120-fold magnification. Last, the germinated single spore with water agar was transferred onto oatmeal tomato agar for growth and preservation. Based on our experience, a skilled technician or student can successfully isolate single spore from over 150 independent diseased samples with nearly no contaminations in a single working day. This approach is also suitable for isolating single spore from other fungal disease samples that produce abundant aerial conidia.
其他摘要	Single spore isolation is a fundamental approach in plant pathology and mycology to isolate and identify plant fungal pathogens from diseased samples. However, routine single spore isolation procedure is time-consuming and has a high risk of contamination by other microorganisms. In this study, we developed a rapid approach for isolating a single spore of the fungal pathogen, Pyricularia oryzae, from rice blast diseased leaves in the paddy field with low potential of contamination. First, rice blast leaves with single lesions were selected in the paddy field, and a single lesion was cut out and pressed and dragged gently across the surface of water agar. Next, a germinated single spore with a barely visible piece of agar was cut out of water agar with a dissecting needle under a stereomicroscope at approximately 120-fold magnification. Last, the germinated single spore with water agar was transferred onto oatmeal tomato agar for growth and preservation. Based on our experience, a skilled technician or student can successfully isolate single spore from over 150 independent diseased samples with nearly no contaminations in a single working day. This approach is also suitable for isolating single spore from other fungal disease samples that produce abundant aerial conidia.
关键词	Pyricularia oryzae single spore isolation rice blast field experiment
收录类别	CSCD
语种	英语
资助项目	[earmarked fund for China Agricultural Research System] ; [National Key Research and Development Program of China]
CSCD记录号	CSCD:6517127
引用统计
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/149259
专题	中国科学院金属研究所
作者单位	1.中国科学院亚热带农业生态研究所 2.中国科学院金属研究所 3.Liaoning Acad Agr Sci, Saline Alkali Land Utilizat & Res Institute, Panjin 124010, Peoples R China
推荐引用方式 GB/T 7714	Fei Liwang,Lu Wenbo,Xu Xiaozhou,et al. A rapid approach for isolating a single fungal spore from rice blast diseased leaves[J]. JOURNAL OF INTEGRATIVE AGRICULTURE,2019,18(6):1415-1418.
APA	Fei Liwang.,Lu Wenbo.,Xu Xiaozhou.,Yan Fengcheng.,Zhang Liwei.,...&Peng Youliang.(2019).A rapid approach for isolating a single fungal spore from rice blast diseased leaves.JOURNAL OF INTEGRATIVE AGRICULTURE,18(6),1415-1418.
MLA	Fei Liwang,et al."A rapid approach for isolating a single fungal spore from rice blast diseased leaves".JOURNAL OF INTEGRATIVE AGRICULTURE 18.6(2019):1415-1418.