Overexpression of the neuroglobin gene delivered by ultrasound-targeted microbubble destruction protects SH-SY5Y cells against cobalt chloride induced hypoxia

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	Overexpression of the neuroglobin gene delivered by ultrasound-targeted microbubble destruction protects SH-SY5Y cells against cobalt chloride induced hypoxia
其他题名	Overexpression of the neuroglobin gene delivered by ultrasound-targeted microbubble destruction protects SH-SY5Y cells against cobalt chloride induced hypoxia
	Yang Qian 1; Gao Dianwen 1; Nie Qingzhu 1; Cai Zhengang 2; Du Jian 2; Shan Lujuan 3; Liu Yuejian 3
	2011
发表期刊	NEURAL REGENERATION RESEARCH
ISSN	1673-5374
卷号	6 期号:25 页码:1947-1953
摘要	In this study, we examined the effects of neuroglobin gene (Ngb) transfection into SH-SY5Y cells, using ultrasound-targeted microbubble destruction (UTMD), on cobalt chloride-induced hypoxia. With an ultrasound intensity of 0.8 W/cm(2), a 60-second exposure duration, 50% duty cycle, and 20% microbubble concentration, pAcGFP1-C1-Ngb-transfected cells exhibited the highest cell viability and transfection efficiency. The efficiency of plasmid delivery was significantly higher with UTMD than transfection with plasmid alone, transfection with plasmid using microbubbles, or transfection of plasmid by ultrasound. In addition, during cobalt chloride-induced hypoxia, caspase-3 activity in pAcGFP1-C1-Ngb-transfected cells was significantly lower than in untransfected cells. Ngb protein and mRNA expression were significantly higher in cells transfected by UTMD than in cells transfected with the other methods. These results demonstrate that UTMD can very efficiently mediate exogenous gene delivery, and that Ngb overexpression protects cells against cobalt chloride-induced hypoxia.
其他摘要	In this study, we examined the effects of neuroglobin gene (Ngb) transfection into SH-SY5Y cells, using ultrasound-targeted microbubble destruction (UTMD), on cobalt chloride-induced hypoxia. With an ultrasound intensity of 0.8 W/cm2, a 60-second exposure duration, 50% duty cycle, and 20% microbubble concentration, pAcGFP1-C1-Ngb-transfected cells exhibited the highest cell viability and transfection efficiency. The efficiency of plasmid delivery was significantly higher with UTMD than transfection with plasmid alone, transfection with plasmid using microbubbles, or transfection of plasmid by ultrasound. In addition, during cobalt chloride-induced hypoxia, caspase-3 activity in pAcGFP1-C1-Ngb-transfected cells was significantly lower than in untransfected cells. Ngb protein and mRNA expression were significantly higher in cells transfected by UTMD than in cells transfected with the other methods. These results demonstrate that UTMD can very efficiently mediate exogenous gene delivery, and that Ngb overexpression protects cells against cobalt chloride-induced hypoxia
关键词	OXIDATIVE STRESS IN-VITRO EXPRESSION THERAPY BRAIN MICE TRANSCRIPTION ADAPTATION IMPROVES PROGRESS ultrasound-targeted microbubble destruction neuroglobin gene therapy recombinant plasmid SH-SY5Y cells neural regeneration
收录类别	CSCD
语种	英语
CSCD记录号	CSCD:4363499
引用统计
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/149677
专题	中国科学院金属研究所
作者单位	1.中国科学院金属研究所 2.Dalian Med University, Hosp 1, Dept Gen Surg, Dalian 116021, Liaoning Provin, Peoples R China 3.Dalian Med University, Hosp 1, Expt Res Ctr, Dalian 116021, Liaoning Provin, Peoples R China
推荐引用方式 GB/T 7714	Yang Qian,Gao Dianwen,Nie Qingzhu,et al. Overexpression of the neuroglobin gene delivered by ultrasound-targeted microbubble destruction protects SH-SY5Y cells against cobalt chloride induced hypoxia[J]. NEURAL REGENERATION RESEARCH,2011,6(25):1947-1953.
APA	Yang Qian.,Gao Dianwen.,Nie Qingzhu.,Cai Zhengang.,Du Jian.,...&Liu Yuejian.(2011).Overexpression of the neuroglobin gene delivered by ultrasound-targeted microbubble destruction protects SH-SY5Y cells against cobalt chloride induced hypoxia.NEURAL REGENERATION RESEARCH,6(25),1947-1953.
MLA	Yang Qian,et al."Overexpression of the neuroglobin gene delivered by ultrasound-targeted microbubble destruction protects SH-SY5Y cells against cobalt chloride induced hypoxia".NEURAL REGENERATION RESEARCH 6.25(2011):1947-1953.