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Overexpression of the neuroglobin gene delivered by ultrasound-targeted microbubble destruction protects SH-SY5Y cells against cobalt chloride induced hypoxia
Alternative TitleOverexpression of the neuroglobin gene delivered by ultrasound-targeted microbubble destruction protects SH-SY5Y cells against cobalt chloride induced hypoxia
Yang Qian1; Gao Dianwen1; Nie Qingzhu1; Cai Zhengang2; Du Jian2; Shan Lujuan3; Liu Yuejian3
2011
Source PublicationNEURAL REGENERATION RESEARCH
ISSN1673-5374
Volume6Issue:25Pages:1947-1953
AbstractIn this study, we examined the effects of neuroglobin gene (Ngb) transfection into SH-SY5Y cells, using ultrasound-targeted microbubble destruction (UTMD), on cobalt chloride-induced hypoxia. With an ultrasound intensity of 0.8 W/cm(2), a 60-second exposure duration, 50% duty cycle, and 20% microbubble concentration, pAcGFP1-C1-Ngb-transfected cells exhibited the highest cell viability and transfection efficiency. The efficiency of plasmid delivery was significantly higher with UTMD than transfection with plasmid alone, transfection with plasmid using microbubbles, or transfection of plasmid by ultrasound. In addition, during cobalt chloride-induced hypoxia, caspase-3 activity in pAcGFP1-C1-Ngb-transfected cells was significantly lower than in untransfected cells. Ngb protein and mRNA expression were significantly higher in cells transfected by UTMD than in cells transfected with the other methods. These results demonstrate that UTMD can very efficiently mediate exogenous gene delivery, and that Ngb overexpression protects cells against cobalt chloride-induced hypoxia.
Other AbstractIn this study, we examined the effects of neuroglobin gene (Ngb) transfection into SH-SY5Y cells, using ultrasound-targeted microbubble destruction (UTMD), on cobalt chloride-induced hypoxia. With an ultrasound intensity of 0.8 W/cm2, a 60-second exposure duration, 50% duty cycle, and 20% microbubble concentration, pAcGFP1-C1-Ngb-transfected cells exhibited the highest cell viability and transfection efficiency. The efficiency of plasmid delivery was significantly higher with UTMD than transfection with plasmid alone, transfection with plasmid using microbubbles, or transfection of plasmid by ultrasound. In addition, during cobalt chloride-induced hypoxia, caspase-3 activity in pAcGFP1-C1-Ngb-transfected cells was significantly lower than in untransfected cells. Ngb protein and mRNA expression were significantly higher in cells transfected by UTMD than in cells transfected with the other methods. These results demonstrate that UTMD can very efficiently mediate exogenous gene delivery, and that Ngb overexpression protects cells against cobalt chloride-induced hypoxia
KeywordOXIDATIVE STRESS IN-VITRO EXPRESSION THERAPY BRAIN MICE TRANSCRIPTION ADAPTATION IMPROVES PROGRESS ultrasound-targeted microbubble destruction neuroglobin gene therapy recombinant plasmid SH-SY5Y cells neural regeneration
Indexed ByCSCD
Language英语
CSCD IDCSCD:4363499
Citation statistics
Document Type期刊论文
Identifierhttp://ir.imr.ac.cn/handle/321006/149677
Collection中国科学院金属研究所
Affiliation1.中国科学院金属研究所
2.Dalian Med University, Hosp 1, Dept Gen Surg, Dalian 116021, Liaoning Provin, Peoples R China
3.Dalian Med University, Hosp 1, Expt Res Ctr, Dalian 116021, Liaoning Provin, Peoples R China
Recommended Citation
GB/T 7714
Yang Qian,Gao Dianwen,Nie Qingzhu,et al. Overexpression of the neuroglobin gene delivered by ultrasound-targeted microbubble destruction protects SH-SY5Y cells against cobalt chloride induced hypoxia[J]. NEURAL REGENERATION RESEARCH,2011,6(25):1947-1953.
APA Yang Qian.,Gao Dianwen.,Nie Qingzhu.,Cai Zhengang.,Du Jian.,...&Liu Yuejian.(2011).Overexpression of the neuroglobin gene delivered by ultrasound-targeted microbubble destruction protects SH-SY5Y cells against cobalt chloride induced hypoxia.NEURAL REGENERATION RESEARCH,6(25),1947-1953.
MLA Yang Qian,et al."Overexpression of the neuroglobin gene delivered by ultrasound-targeted microbubble destruction protects SH-SY5Y cells against cobalt chloride induced hypoxia".NEURAL REGENERATION RESEARCH 6.25(2011):1947-1953.
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