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microRNA-218 suppresses the proliferation, invasion and promotes apoptosis of pancreatic cancer cells by targeting HMGB1
Alternative TitlemicroRNA-218 suppresses the proliferation, invasion and promotes apoptosis of pancreatic cancer cells by targeting HMGB1
Liu Zhe; Xu Yuanhong; Long Jin; Guo Kejian; Ge Chunlin; Du Ruixia
2015
Source PublicationCHINESE JOURNAL OF CANCER RESEARCH
ISSN1000-9604
Volume27Issue:3Pages:247-257
AbstractObjective: To detect the expression profiles of microRNA-218 (miR-218) in human pancreatic cancer tissue (PCT) and cells and their effects on the biological features of human pancreatic cancer cell line PANC-1 and observe the effect of miR-218 on the expression of the target gene high mobility group box 1 (HMGB1), with an attempt to provide new treatment methods and strategies for pancreatic cancer.
Other AbstractObjective To detect the expression profiles of microRNA-218 (miR-218) in human pancreatic cancer tissue (PCT) and cells and their effects on the biological features of human pancreatic cancer cell line PANC-1 and observe the effect of miR-218 on the expression of the target gene high mobility group box 1 ( HMGB1 ), with an attempt to provide new treatment methods and strategies for pancreatic cancer. Methods The expressions of miR-218 in PCT and normal pancreas tissue as well as in various pancreatic cancer cell lines including AsPC-1, BxPC-3, and PANC-1 were determined with quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR). The change of miR-218 expression in PANC-1 cells was detected using qRT-PCT after the transfection of miR-218 mimic for 48 h. Cell Counting Kit-8 (CCK-8) was applied for detecting the effect of miR-218 on the activity of PANC-1 cells. The effects of miR-218 on the proliferation and apoptosis of PANC-1 cells were analyzed using the flow cytometry. The effect of miR-218 on the migration of PANC-1 cells was detected using the Trans-well migration assay. The HMGB1 was found to be a target gene of miR-218 by luciferase reporter assay, and the effect of miR-218 on the expression of HMGB1 protein in cells were determined using Western blotting. Results As shown by qRT-PCR, the expressions of miR-218 in PCT and in pancreatic cancer cell line significantly decreased when compared with the normal pancreatic tissue (NPT) (P<0.01). Compared with the control group, the miR-218 expression significantly increased in the PANC-1 group after the transfection of miR-218 mimic for 48 h (P<0.01). Growth curve showed that the cell viability significantly dropped after the overexpression of miR-218 in the PANC-1 cells for two days (P<0.05). Flow cytometry showed that the S-phase fraction significantly dropped after the overexpression of miR-218 (P<0.01) and the percentage of apoptotic cells significantly increased (P<0.01). As shown by the Trans-well migration assay, the enhanced miR-218 expression was associated with a significantly lower number of cells that passed through a Transwell chamber (P<0.01). Luciferase reporter assay showed that, compared with the control group, the relative luciferase activity significantly decreased in the miR-218 mimic group (P<0.01). As shown by the Western blotting, compared with the control group, the HMGB1 protein expression significantly decreased in the PANC-1 group after the transfection of miR-218 mimic for 48 h (P<0.01). Conclusions The miR-218 expression decreases in human PCT and cell lines. miR-218 can negatively regulate the HMGB1 protein expression and inhibit the proliferation and invasion of pancreatic cancer cells. A treatment strategy by enhancing the miR-218 expression may benefit the patients with pancreatic cancer.
KeywordGROUP BOX 1 EXPRESSION GENE ADENOCARCINOMA PROGRESSION INHIBITORS BIOMARKERS MANAGEMENT MIGRATION OPTIONS Pancreatic cancer microRNA-218 (miR-218) proliferation apoptosis high mobility group box 1 (HMGB1)
Indexed ByCSCD
Language英语
Funding Project[Liaoning Provincial Department of education science research project] ; [Liaoning province science and technology plan project] ; [The Shenyang Municipal Science and technology project]
CSCD IDCSCD:5467264
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Document Type期刊论文
Identifierhttp://ir.imr.ac.cn/handle/321006/149685
Collection中国科学院金属研究所
Affiliation中国科学院金属研究所
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GB/T 7714
Liu Zhe,Xu Yuanhong,Long Jin,et al. microRNA-218 suppresses the proliferation, invasion and promotes apoptosis of pancreatic cancer cells by targeting HMGB1[J]. CHINESE JOURNAL OF CANCER RESEARCH,2015,27(3):247-257.
APA Liu Zhe,Xu Yuanhong,Long Jin,Guo Kejian,Ge Chunlin,&Du Ruixia.(2015).microRNA-218 suppresses the proliferation, invasion and promotes apoptosis of pancreatic cancer cells by targeting HMGB1.CHINESE JOURNAL OF CANCER RESEARCH,27(3),247-257.
MLA Liu Zhe,et al."microRNA-218 suppresses the proliferation, invasion and promotes apoptosis of pancreatic cancer cells by targeting HMGB1".CHINESE JOURNAL OF CANCER RESEARCH 27.3(2015):247-257.
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