Effect of allitridi on inducing mitotic arrest in human gastric cell line SGC-7901 and its possible mechanism

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	Effect of allitridi on inducing mitotic arrest in human gastric cell line SGC-7901 and its possible mechanism
其他题名	Effect of allitridi on inducing mitotic arrest in human gastric cell line SGC-7901 and its possible mechanism
	Chen Tiejun; Ha Minwen; Gong Yuehua; Xu Qian; Yuan Yuan
	2008
发表期刊	CHINESE JOURNAL OF CANCER RESEARCH
ISSN	1000-9604
卷号	20 期号:2 页码:126-132
摘要	Objective: To learn the effect of allitridi on inducing mitotic arrest in human gastric cell line SGC-7901 and its possible mechanisms. Methods: We treated SGC-7901 cells with allitridi, and observed the proliferation inhibitory rate with MTT colometric assay, changes of cell cycle using flow cytometry and Switzerland-Giemsa's staining, and morphologic changes of the microtubule structure and location changes of cyclin B(1) expression using immunofluorescence and confocal laser scanning microscope. Furthermore, the expression of cyclin B(1) was analyzed quantitatively using Leica confocal software. Results: SGC-7901 cells were inhibited after exposure to allitridi and the IC(50) was 7.2 mu g/ml for 24 h, 20 mu g/ml for 72 h. When the cells were treated with allitridi at concentrations of 3, 6, and 9 mu g/ml for 24 h respectively, there was a declining tendency in the percentage of G(0)/G(1) cell but an increasing tendency in G(2)/M cell in the allitridi treated group compared with that of control (P < 0.01). When cells were treated allitridi at concentration of 6 mu g/ml for 24 h, its mitotic index was much higher (P < 0.01) than that of control, suggesting that allitridi caused arrest of gastric cancer cells in M phase. The cells were treated with allitridi became more shrunken and nepheloid, in which the microtubule networks disappeared, while the control cell exhibited an intact microtubule network. Contrasting with normal existence mainly in the cytoplasm, the cyclin B(1) was expressed more significantly and concentrated in the nucleus after exposure to allitridi. Fluorescence intensity of cyclin B1 protein in cells treated with allitridi was much more higher than that of control (P < 0.001). Conclusion: Allitridi can induce arrest of SGC-7901 cells in M phase, probably through enhancing microtubule depolymerization by elevating the expression of cyclin B(1).
其他摘要	Objective:To learn the effect of allitridi on inducing mitotic arrest in human gastric cell line SGC-7901 and its possible mechanisms.Methods:We treated SGC-7901 cells with allitridi,and observed the proliferation inhibitory rate with MTT colometric assay,changes of cell cycle using flow cytometry and Switzerland-Giemsa's staining,and morphologic changes of the microtubule structure and location changes of cyclin B_1 expression using immunofluorescence and confocal laser scanning microscope.Furthermore,the expression of cyclin B_1 was analyzed quantitatively using Leica confocal software.Results: SGC-7901 cells were inhibited after exposure to allitridi and the IC_(50)was 7.2μg/ml for 24h,20μg/ml for 72h.When the cells were treated with allitridi at concentrations of 3,6,and 9μg/ml for 24h respectively,there was a declining tendency in the percentage of G_0/G_1 cell but an increasing tendency in G_2/M cell in the allitridi treated group compared with that of control (P<0.01).When cells were treated allitridi at concentration of 6μg/ml for 24h,its mitotic index was much higher(P<0.01) than that of control,suggesting that allitridi caused arrest of gastric cancer cells in M phase.The cells were treated with allitridi became more shrunken and nepheloid,in which the microtubule networks disappeared,while the control cell exhibited an intact microtubule network.Contrasting with normal existence mainly in the cytoplasm,the cyclin B_1 was expressed more significantly and concentrated in the nucleus after exposure to allitridi.Fluorescence intensity of cyclin B1 protein in cells treated with allitridi was much more higher than that of control(P<0.001).Conclusion:Allitridi can induce arrest of SGC-7901 cells in M phase,probably through enhancing microtubule depolymerization by elevating the expression of cyclin B_1.
关键词	APOPTOSIS EXPRESSION CARCINOMA CANCER allitridi stomach neoplasms cell cycle P34 cdc2/cyclin B(1) microtubule
收录类别	CSCD
语种	英语
CSCD记录号	CSCD:3299654
引用统计
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/149796
专题	中国科学院金属研究所
作者单位	中国科学院金属研究所
推荐引用方式 GB/T 7714	Chen Tiejun,Ha Minwen,Gong Yuehua,et al. Effect of allitridi on inducing mitotic arrest in human gastric cell line SGC-7901 and its possible mechanism[J]. CHINESE JOURNAL OF CANCER RESEARCH,2008,20(2):126-132.
APA	Chen Tiejun,Ha Minwen,Gong Yuehua,Xu Qian,&Yuan Yuan.(2008).Effect of allitridi on inducing mitotic arrest in human gastric cell line SGC-7901 and its possible mechanism.CHINESE JOURNAL OF CANCER RESEARCH,20(2),126-132.
MLA	Chen Tiejun,et al."Effect of allitridi on inducing mitotic arrest in human gastric cell line SGC-7901 and its possible mechanism".CHINESE JOURNAL OF CANCER RESEARCH 20.2(2008):126-132.