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Generation and characterization of c305, a murine neutralizing scFv antibody that can inhibit BLyS binding to its receptor BCMA
Alternative TitleGeneration and Characterization of C305, a Murine Neutralizing scFv Antibody That Can Inhibit BLyS Binding to Its Receptor BCMA
Liu MY1; Han W1; Ding YL1; Zhou TH1; Tian RY1; Yang SL1; Liu H1; Gong Y1
2005
Source PublicationACTA BIOCHIMICA ET BIOPHYSICA SINICA
ISSN1672-9145
Volume37Issue:6Pages:415-420
AbstractB-lymphocyte stimulator (BLyS) is a member of the tumor necrosis factor (TNF) family and a key regulator of B cell response. Neutralizing single-chain fragment variable (scFv) antibody against BLyS binding to its receptor BCMA has the potential to play a prominent role in autoimmune disease therapy. A phage display scFv library constructed on pIII protein of M13 filamentous phage was screened using BLyS. After five rounds of panning, their binding activity was characterized by phage-ELISA. Nucleotide sequencing revealed that at least two different scFv gene fragments (C305 and D416) were obtained. The two different scFv gene fragments were expressed to obtain the soluble scFv antibodies, then the soluble scFv antibodies were characterized by means of competitive ELISA and in vitro neutralization assay. The results indicated that C305 is the neutralizing scFv antibody that can inhibit BLyS binding to its receptor BCMA.
Other AbstractB-lymphocyte stimulator (BLyS) is a member of the tumor necrosis factor (TNF) family and a key regulator of B cell response. Neutralizing single-chain fragment variable (scFv) antibody against BLyS binding to its receptor BCMA has the potential to play a prominent role in autoimmune disease therapy. A phage display scFv library constructed on pIII protein of M13 filamentous phage was screened using BLyS. After five rounds of panning, their binding activity was characterized by phage-ELISA. Nucleotide sequencing revealed that at least two different scFv gene fragments (C305 and D416) were obtained. The two different scFv gene fragments were expressed to obtain the soluble scFv antibodies, then the soluble scFv antibodies were characterized by means of competitive ELISA and in vitro neutralization assay. The results indicated that C305 is the neutralizing scFv antibody that can inhibit BLyS binding to its receptor BCMA.
KeywordB-LYMPHOCYTE STIMULATOR NECROSIS-FACTOR FAMILY AUTOIMMUNE-DISEASE SJOGRENS-SYNDROME CELL MATURATION TNF FAMILY BAFF MEMBER TACI MICE phage display neutralizing scFv antibody BLyS
Indexed ByCSCD
Language英语
CSCD IDCSCD:2147594
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Document Type期刊论文
Identifierhttp://ir.imr.ac.cn/handle/321006/149984
Collection中国科学院金属研究所
Affiliation1.中国科学院
2.暨南大学
3.中国科学院金属研究所
Recommended Citation
GB/T 7714
Liu MY,Han W,Ding YL,et al. Generation and characterization of c305, a murine neutralizing scFv antibody that can inhibit BLyS binding to its receptor BCMA[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2005,37(6):415-420.
APA Liu MY.,Han W.,Ding YL.,Zhou TH.,Tian RY.,...&Gong Y.(2005).Generation and characterization of c305, a murine neutralizing scFv antibody that can inhibit BLyS binding to its receptor BCMA.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,37(6),415-420.
MLA Liu MY,et al."Generation and characterization of c305, a murine neutralizing scFv antibody that can inhibit BLyS binding to its receptor BCMA".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 37.6(2005):415-420.
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