| Expression, purification, and characterization of a thermophilic neutral protease from Bacillus stearothermophilus in Bacillus subtilis | |
| Alternative Title | Expression, purification, and characterization of a thermophilic neutral protease from Bacillus stearothermophilus in Bacillus subtilis |
| Zhang Min1; Zhao Cong1; Du LianXiang1; Lu FuPing1; Gao Chen3 | |
| 2008 | |
| Source Publication | SCIENCE IN CHINA SERIES C-LIFE SCIENCES
 |
| ISSN | 1006-9305 |
| Volume | 51Issue:1Pages:52-59 |
| Abstract | The gene coding for a thermophilic neutral protease from Bacillus stearothermophilus was expressed in Bacillus subtilis DB104, under the control of the sacB gene promoter. This was followed by either the native signal peptide sequence of this protease or the signal peptide sequence of the sacB gene. The protease was purified 3.8-fold, with a specific activity of 16530 U mg(-1). As analyzed by SDS-PAGE, the molecular mass of the expressed protease was about 35 kDa, and the optimal temperature and pH of the protease were 65 degrees C and 7.5, respectively. Moreover, it still had about 80% activity after 1 h reaction at 65 degrees C. |
| Other Abstract | The gene coding for a thermophilic neutral protease from Bacillus stearothermophilus was expressed in Bacillus subtilis DB104, under the control of the sacB gene promoter. This was followed by either the native signal peptide sequence of this protease or the signal peptide sequence of the sacB gene. The protease was purified 3.8-fold, with a specific activity of 16530 U mg~(-1). As analyzed by SDS-PAGE, the molecular mass of the expressed protease was about 35 kDa, and the optimal temperature and pH of the protease were 65℃ and 7.5, respectively. Moreover, it still had about 80% activity after 1 h reaction at 65℃. |
| Keyword | GENE CLONING LICHENIFORMIS SECRETION PLASMID ENZYME Bacillus subtilis thermophilic neutral protease expression purification characterization |
| Indexed By | CSCD |
| Language | 英语 |
| CSCD ID | CSCD:3585990 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.imr.ac.cn/handle/321006/150271 |
| Collection | 中国科学院金属研究所 |
| Affiliation | 1.Tianjin Univ Sci & Technol, Coll Biotechnology, Tianjin 300457, Peoples R China 2.中国科学院金属研究所 3.南开大学 |
| Recommended Citation GB/T 7714 | Zhang Min,Zhao Cong,Du LianXiang,et al. Expression, purification, and characterization of a thermophilic neutral protease from Bacillus stearothermophilus in Bacillus subtilis[J]. SCIENCE IN CHINA SERIES C-LIFE SCIENCES,2008,51(1):52-59. |
| APA | Zhang Min,Zhao Cong,Du LianXiang,Lu FuPing,&Gao Chen.(2008).Expression, purification, and characterization of a thermophilic neutral protease from Bacillus stearothermophilus in Bacillus subtilis.SCIENCE IN CHINA SERIES C-LIFE SCIENCES,51(1),52-59. |
| MLA | Zhang Min,et al."Expression, purification, and characterization of a thermophilic neutral protease from Bacillus stearothermophilus in Bacillus subtilis".SCIENCE IN CHINA SERIES C-LIFE SCIENCES 51.1(2008):52-59. |
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