Expression, purification, and characterization of a thermophilic neutral protease from Bacillus stearothermophilus in Bacillus subtilis

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	Expression, purification, and characterization of a thermophilic neutral protease from Bacillus stearothermophilus in Bacillus subtilis
其他题名	Expression, purification, and characterization of a thermophilic neutral protease from Bacillus stearothermophilus in Bacillus subtilis
	Zhang Min 1; Zhao Cong 1; Du LianXiang 1; Lu FuPing 1; Gao Chen 3
	2008
发表期刊	SCIENCE IN CHINA SERIES C-LIFE SCIENCES
ISSN	1006-9305
卷号	51 期号:1 页码:52-59
摘要	The gene coding for a thermophilic neutral protease from Bacillus stearothermophilus was expressed in Bacillus subtilis DB104, under the control of the sacB gene promoter. This was followed by either the native signal peptide sequence of this protease or the signal peptide sequence of the sacB gene. The protease was purified 3.8-fold, with a specific activity of 16530 U mg(-1). As analyzed by SDS-PAGE, the molecular mass of the expressed protease was about 35 kDa, and the optimal temperature and pH of the protease were 65 degrees C and 7.5, respectively. Moreover, it still had about 80% activity after 1 h reaction at 65 degrees C.
其他摘要	The gene coding for a thermophilic neutral protease from Bacillus stearothermophilus was expressed in Bacillus subtilis DB104, under the control of the sacB gene promoter. This was followed by either the native signal peptide sequence of this protease or the signal peptide sequence of the sacB gene. The protease was purified 3.8-fold, with a specific activity of 16530 U mg~(-1). As analyzed by SDS-PAGE, the molecular mass of the expressed protease was about 35 kDa, and the optimal temperature and pH of the protease were 65℃ and 7.5, respectively. Moreover, it still had about 80% activity after 1 h reaction at 65℃.
关键词	GENE CLONING LICHENIFORMIS SECRETION PLASMID ENZYME Bacillus subtilis thermophilic neutral protease expression purification characterization
收录类别	CSCD
语种	英语
CSCD记录号	CSCD:3585990
引用统计
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/150271
专题	中国科学院金属研究所
作者单位	1.Tianjin Univ Sci & Technol, Coll Biotechnology, Tianjin 300457, Peoples R China 2.中国科学院金属研究所 3.南开大学
推荐引用方式 GB/T 7714	Zhang Min,Zhao Cong,Du LianXiang,et al. Expression, purification, and characterization of a thermophilic neutral protease from Bacillus stearothermophilus in Bacillus subtilis[J]. SCIENCE IN CHINA SERIES C-LIFE SCIENCES,2008,51(1):52-59.
APA	Zhang Min,Zhao Cong,Du LianXiang,Lu FuPing,&Gao Chen.(2008).Expression, purification, and characterization of a thermophilic neutral protease from Bacillus stearothermophilus in Bacillus subtilis.SCIENCE IN CHINA SERIES C-LIFE SCIENCES,51(1),52-59.
MLA	Zhang Min,et al."Expression, purification, and characterization of a thermophilic neutral protease from Bacillus stearothermophilus in Bacillus subtilis".SCIENCE IN CHINA SERIES C-LIFE SCIENCES 51.1(2008):52-59.