| Serologic and molecular survey for major viral pathogens in grazing hybrid wild boars in Northeast China | |
| Alternative Title | Serologic and molecular survey for major viral pathogens in grazing hybrid wild boars in Northeast China |
| Guo Huancheng1; Ren Zhaowen1; Ding Meiming2; Xiao Wanjun1; Peng Peng3; He Biao1; Feng Ye1; Liu Yan1; Li Xingyu1; Cai Jianqiu1; Zhang Bikai1; Luo Qinghua1; Tu Changchun1 | |
| 2019 | |
| Source Publication | JOURNAL OF INTEGRATIVE AGRICULTURE
 |
| ISSN | 2095-3119 |
| Volume | 18Issue:9Pages:2133-2140 |
| Abstract | Hybrid wild boar husbandry is an important component of livestock production in Northeast China. However, the current disease situation of these animals is largely unknown due to a lack of disease surveillance. The present study was conducted to determine the prevalence of several important viral diseases in the hybrid wild boar population of Northeast China. Between September 2015 to December 2016, 169 blood and 61 tissue samples were collected from apparently healthy hybrid wild boars from farms in Jilin, Inner Mongolia and Heilongjiang provinces. ELISA detected serum antibodies against classical swine fever virus (CSFV), porcine reproductive and respiratory syndrome virus (PRRSV), pseudorabies virus (PRV), porcine circovirus type 2 (PCV2) and Japanese encephalitis virus (JEV), but not against African swine fever virus (ASFV), with PCV2 having the highest seropositive rate (87.2-100% in different farms). RT-PCR or PCR performed on the processed samples detected only PCV2, with 33.1% (56/169) of blood samples and 32.8% (20/61) of spleen samples being positive, respectively, indicating widespread PCV2 infection in hybrid wild boars. Phylogenetic analysis of 15 PCV2 ORF2 sequences showed that they belong to genotypes PCV2a, PCV2b and PCV2d, with nucleotide and deduced amino acid homologies of 88.5-100% and 88.1-100%, respectively. |
| Other Abstract | Hybrid wild boar husbandry is an important component of livestock production in Northeast China. However, the current disease situation of these animals is largely unknown due to a lack of disease surveillance. The present study was conducted to determine the prevalence of several important viral diseases in the hybrid wild boar population of Northeast China. Between September 2015 to December 2016, 169 blood and 61 tissue samples were collected from apparently healthy hybrid wild boars from farms in Jilin, Inner Mongolia and Heilongjiang provinces. ELISA detected serum antibodies against classical swine fever virus (CSFV), porcine reproductive and respiratory syndrome virus (PRRSV), pseudorabies virus (PRV), porcine circovirus type 2 (PCV2) and Japanese encephalitis virus (JEV), but not against African swine fever virus (ASFV), with PCV2 having the highest seropositive rate (87.2–100% in different farms). RT-PCR or PCR performed on the processed samples detected only PCV2, with 33.1% (56/169) of blood samples and 32.8% (20/61) of spleen samples being positive, respectively, indicating widespread PCV2 infection in hybrid wild boars. Phylogenetic analysis of 15 PCV2 ORF2 sequences showed that they belong to genotypes PCV2a, PCV2b and PCV2d, with nucleotide and deduced amino acid homologies of 88.5–100% and 88.1–100%, respectively. |
| Keyword | SWINE-FEVER VIRUS PORCINE CIRCOVIRUS TYPE-2 JAPANESE ENCEPHALITIS TRANSMISSION PSEUDORABIES EMERGENCE DISEASES disease transmission hybrid wild boars Northeast China serological and molecular survey viral diseases |
| Indexed By | CSCD |
| Language | 英语 |
| Funding Project | [National Key Research and Development Program of China] ; [Wildlife Borne Infectious Diseases Monitoring Project of the State Forestry Administration (SFA) of China] |
| CSCD ID | CSCD:6579808 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.imr.ac.cn/handle/321006/150455 |
| Collection | 中国科学院金属研究所 |
| Affiliation | 1.中山大学 2.Greater Xingan Mt Forestry Grp Corp, Div Wildlife Conservat & Nat Reserve Management, Jiagedaqi 165000, Peoples R China 3.中国科学院金属研究所 4.扬州大学 |
| Recommended Citation GB/T 7714 | Guo Huancheng,Ren Zhaowen,Ding Meiming,et al. Serologic and molecular survey for major viral pathogens in grazing hybrid wild boars in Northeast China[J]. JOURNAL OF INTEGRATIVE AGRICULTURE,2019,18(9):2133-2140. |
| APA | Guo Huancheng.,Ren Zhaowen.,Ding Meiming.,Xiao Wanjun.,Peng Peng.,...&Tu Changchun.(2019).Serologic and molecular survey for major viral pathogens in grazing hybrid wild boars in Northeast China.JOURNAL OF INTEGRATIVE AGRICULTURE,18(9),2133-2140. |
| MLA | Guo Huancheng,et al."Serologic and molecular survey for major viral pathogens in grazing hybrid wild boars in Northeast China".JOURNAL OF INTEGRATIVE AGRICULTURE 18.9(2019):2133-2140. |
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