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Serologic and molecular survey for major viral pathogens in grazing hybrid wild boars in Northeast China
Alternative TitleSerologic and molecular survey for major viral pathogens in grazing hybrid wild boars in Northeast China
Guo Huancheng1; Ren Zhaowen1; Ding Meiming2; Xiao Wanjun1; Peng Peng3; He Biao1; Feng Ye1; Liu Yan1; Li Xingyu1; Cai Jianqiu1; Zhang Bikai1; Luo Qinghua1; Tu Changchun1
2019
Source PublicationJOURNAL OF INTEGRATIVE AGRICULTURE
ISSN2095-3119
Volume18Issue:9Pages:2133-2140
AbstractHybrid wild boar husbandry is an important component of livestock production in Northeast China. However, the current disease situation of these animals is largely unknown due to a lack of disease surveillance. The present study was conducted to determine the prevalence of several important viral diseases in the hybrid wild boar population of Northeast China. Between September 2015 to December 2016, 169 blood and 61 tissue samples were collected from apparently healthy hybrid wild boars from farms in Jilin, Inner Mongolia and Heilongjiang provinces. ELISA detected serum antibodies against classical swine fever virus (CSFV), porcine reproductive and respiratory syndrome virus (PRRSV), pseudorabies virus (PRV), porcine circovirus type 2 (PCV2) and Japanese encephalitis virus (JEV), but not against African swine fever virus (ASFV), with PCV2 having the highest seropositive rate (87.2-100% in different farms). RT-PCR or PCR performed on the processed samples detected only PCV2, with 33.1% (56/169) of blood samples and 32.8% (20/61) of spleen samples being positive, respectively, indicating widespread PCV2 infection in hybrid wild boars. Phylogenetic analysis of 15 PCV2 ORF2 sequences showed that they belong to genotypes PCV2a, PCV2b and PCV2d, with nucleotide and deduced amino acid homologies of 88.5-100% and 88.1-100%, respectively.
Other AbstractHybrid wild boar husbandry is an important component of livestock production in Northeast China. However, the current disease situation of these animals is largely unknown due to a lack of disease surveillance. The present study was conducted to determine the prevalence of several important viral diseases in the hybrid wild boar population of Northeast China. Between September 2015 to December 2016, 169 blood and 61 tissue samples were collected from apparently healthy hybrid wild boars from farms in Jilin, Inner Mongolia and Heilongjiang provinces. ELISA detected serum antibodies against classical swine fever virus (CSFV), porcine reproductive and respiratory syndrome virus (PRRSV), pseudorabies virus (PRV), porcine circovirus type 2 (PCV2) and Japanese encephalitis virus (JEV), but not against African swine fever virus (ASFV), with PCV2 having the highest seropositive rate (87.2–100% in different farms). RT-PCR or PCR performed on the processed samples detected only PCV2, with 33.1% (56/169) of blood samples and 32.8% (20/61) of spleen samples being positive, respectively, indicating widespread PCV2 infection in hybrid wild boars. Phylogenetic analysis of 15 PCV2 ORF2 sequences showed that they belong to genotypes PCV2a, PCV2b and PCV2d, with nucleotide and deduced amino acid homologies of 88.5–100% and 88.1–100%, respectively.
KeywordSWINE-FEVER VIRUS PORCINE CIRCOVIRUS TYPE-2 JAPANESE ENCEPHALITIS TRANSMISSION PSEUDORABIES EMERGENCE DISEASES disease transmission hybrid wild boars Northeast China serological and molecular survey viral diseases
Indexed ByCSCD
Language英语
Funding Project[National Key Research and Development Program of China] ; [Wildlife Borne Infectious Diseases Monitoring Project of the State Forestry Administration (SFA) of China]
CSCD IDCSCD:6579808
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Document Type期刊论文
Identifierhttp://ir.imr.ac.cn/handle/321006/150457
Collection中国科学院金属研究所
Affiliation1.中山大学
2.Greater Xingan Mt Forestry Grp Corp, Div Wildlife Conservat & Nat Reserve Management, Jiagedaqi 165000, Peoples R China
3.中国科学院金属研究所
4.扬州大学
Recommended Citation
GB/T 7714
Guo Huancheng,Ren Zhaowen,Ding Meiming,et al. Serologic and molecular survey for major viral pathogens in grazing hybrid wild boars in Northeast China[J]. JOURNAL OF INTEGRATIVE AGRICULTURE,2019,18(9):2133-2140.
APA Guo Huancheng.,Ren Zhaowen.,Ding Meiming.,Xiao Wanjun.,Peng Peng.,...&Tu Changchun.(2019).Serologic and molecular survey for major viral pathogens in grazing hybrid wild boars in Northeast China.JOURNAL OF INTEGRATIVE AGRICULTURE,18(9),2133-2140.
MLA Guo Huancheng,et al."Serologic and molecular survey for major viral pathogens in grazing hybrid wild boars in Northeast China".JOURNAL OF INTEGRATIVE AGRICULTURE 18.9(2019):2133-2140.
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