| Solid-phase synthesis of acyl-plasminogen-streptokinase activator composite(APSAC) and its thrombolytic properties | |
| Alternative Title | Solid-phase Synthesis of Acyl-plasminogen-Streptokinase Activator Composite(APSAC) and Its Thrombolytic Properties |
| Teng LR1; Liang F1; Sun H1; Qin Y1; Hong SS1; Chen J1; Liu LY1 | |
| 2004 | |
| Source Publication | CHEMICAL RESEARCH IN CHINESE UNIVERSITIES
 |
| ISSN | 1005-9040 |
| Volume | 20Issue:5Pages:584-587 |
| Abstract | The dialysis method has been traditionally used for the conversion of native human plasminogen (Glu-Hpg) to lys-plasminogen(Lys-Hpg). Here is described a solid-phase synthesis method for. the preparation of an acyl-plasminogen-streptokinase activator complex (APSAC) from Lys-Hpg, streptokinase (SK) and chemical modification agent(4-amidinophenyl-4'-aminobenzoate hydrochloride) with the L-lysine-Sepharose 4B Column as the carrier. The new method significantly increases the product yield and purity over the liquid-phase methods. The APSAC prepared with the new method exhibits a significant thrombolytic effect with a long half-life of about 8.8 h in rabbits. |
| Other Abstract | The dialysis method has been traditionally used for the conversion of native human plasminogen (GluHpg) to lys-plasminogen(Lys-Hpg). Here is described a solid-phase synthesis method for the preparation of an acyl-plasminogen-streptokinase activator complex(APSAC) from Lys-Hpg, streptokinase (SK) and chemical modification agent(4-amidinophenyl-4'-aminobenzoate hydrochloride) with the L-lysine-Sepharose 4B Column as the carrier. The new method significantly increases the product yield and purity over the liquidphase methods. The APSAC prepared with the new method exhibits a significant thrombolytic effect with a long half-life of about 8. 8 h in rabbits. |
| Keyword | CONVERSION acyl-plasminogen-streptokinase activator complex (APSAC) solid-phase synthesis thrombolytic |
| Indexed By | CSCD |
| Language | 英语 |
| CSCD ID | CSCD:1844816 |
| Citation statistics |
Cited Times:1[CSCD]
[CSCD Record]
|
| Document Type | 期刊论文 |
| Identifier | http://ir.imr.ac.cn/handle/321006/151438 |
| Collection | 中国科学院金属研究所 |
| Affiliation | 1.吉林大学 2.Institute Biol Prod 3.中国科学院金属研究所 |
| Recommended Citation GB/T 7714 | Teng LR,Liang F,Sun H,et al. Solid-phase synthesis of acyl-plasminogen-streptokinase activator composite(APSAC) and its thrombolytic properties[J]. CHEMICAL RESEARCH IN CHINESE UNIVERSITIES,2004,20(5):584-587. |
| APA | Teng LR.,Liang F.,Sun H.,Qin Y.,Hong SS.,...&Liu LY.(2004).Solid-phase synthesis of acyl-plasminogen-streptokinase activator composite(APSAC) and its thrombolytic properties.CHEMICAL RESEARCH IN CHINESE UNIVERSITIES,20(5),584-587. |
| MLA | Teng LR,et al."Solid-phase synthesis of acyl-plasminogen-streptokinase activator composite(APSAC) and its thrombolytic properties".CHEMICAL RESEARCH IN CHINESE UNIVERSITIES 20.5(2004):584-587. |
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