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Solid-phase synthesis of acyl-plasminogen-streptokinase activator composite(APSAC) and its thrombolytic properties
Alternative TitleSolid-phase Synthesis of Acyl-plasminogen-Streptokinase Activator Composite(APSAC) and Its Thrombolytic Properties
Teng LR1; Liang F1; Sun H1; Qin Y1; Hong SS1; Chen J1; Liu LY1
2004
Source PublicationCHEMICAL RESEARCH IN CHINESE UNIVERSITIES
ISSN1005-9040
Volume20Issue:5Pages:584-587
AbstractThe dialysis method has been traditionally used for the conversion of native human plasminogen (Glu-Hpg) to lys-plasminogen(Lys-Hpg). Here is described a solid-phase synthesis method for. the preparation of an acyl-plasminogen-streptokinase activator complex (APSAC) from Lys-Hpg, streptokinase (SK) and chemical modification agent(4-amidinophenyl-4'-aminobenzoate hydrochloride) with the L-lysine-Sepharose 4B Column as the carrier. The new method significantly increases the product yield and purity over the liquid-phase methods. The APSAC prepared with the new method exhibits a significant thrombolytic effect with a long half-life of about 8.8 h in rabbits.
Other AbstractThe dialysis method has been traditionally used for the conversion of native human plasminogen (GluHpg) to lys-plasminogen(Lys-Hpg). Here is described a solid-phase synthesis method for the preparation of an acyl-plasminogen-streptokinase activator complex(APSAC) from Lys-Hpg, streptokinase (SK) and chemical modification agent(4-amidinophenyl-4'-aminobenzoate hydrochloride) with the L-lysine-Sepharose 4B Column as the carrier. The new method significantly increases the product yield and purity over the liquidphase methods. The APSAC prepared with the new method exhibits a significant thrombolytic effect with a long half-life of about 8. 8 h in rabbits.
KeywordCONVERSION acyl-plasminogen-streptokinase activator complex (APSAC) solid-phase synthesis thrombolytic
Indexed ByCSCD
Language英语
CSCD IDCSCD:1844816
Citation statistics
Document Type期刊论文
Identifierhttp://ir.imr.ac.cn/handle/321006/151438
Collection中国科学院金属研究所
Affiliation1.吉林大学
2.Institute Biol Prod
3.中国科学院金属研究所
Recommended Citation
GB/T 7714
Teng LR,Liang F,Sun H,et al. Solid-phase synthesis of acyl-plasminogen-streptokinase activator composite(APSAC) and its thrombolytic properties[J]. CHEMICAL RESEARCH IN CHINESE UNIVERSITIES,2004,20(5):584-587.
APA Teng LR.,Liang F.,Sun H.,Qin Y.,Hong SS.,...&Liu LY.(2004).Solid-phase synthesis of acyl-plasminogen-streptokinase activator composite(APSAC) and its thrombolytic properties.CHEMICAL RESEARCH IN CHINESE UNIVERSITIES,20(5),584-587.
MLA Teng LR,et al."Solid-phase synthesis of acyl-plasminogen-streptokinase activator composite(APSAC) and its thrombolytic properties".CHEMICAL RESEARCH IN CHINESE UNIVERSITIES 20.5(2004):584-587.
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