The cellular repressor of E1A-stimulated genes promotes human VSMCs differentiation in vitro mediated by RhoA and SRF

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	The cellular repressor of E1A-stimulated genes promotes human VSMCs differentiation in vitro mediated by RhoA and SRF
其他题名	The Cellular Repressor of E1A-stimulated Genes Promotes Human VSMCs Differentiation In vitro Mediated by RhoA and SRF
	Han YL 1; Xu HM 1; Yan CH 1; Hu Y 1; Kang J 1; Liu HW 1
	2006
发表期刊	PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS
ISSN	1000-3282
卷号	33 期号:5 页码:438-445
摘要	To investigate the mechanism of the cellular repressor of E1A-stimulated genes (CREG) on the differentiation of vascular smooth muscle cells (VSMCs), the human internal thoracic artery cells (named HITASY cells) were stably infected with sense-CREG pLNCX(2)(+)/CREG and antisense-CREG pLXSN(-)/CREG retroviral vectors and positive clone was abtained by G418 selection. The cells infected with pLNCX2(+)/CREG take on the differentiated phenotype and overexpression of CREG can enhance SM alpha-actin expression accompanied with increase of the total RhoA and nuclear serum response factor (SRF) expression detected by immunofluorescence and Western blot. And treated with a selective Rho kinase inhibitor Y-27632, the expression of SM at-actin and nuclear SRF protein induced by CREG was effectively reduced. Meanwhile, the opposite results were observed in the CREG-depression HITASY cells. And immunoprecipitation assays indicate that as a secreted protein, CREG can be detected in the media of pLNCX2(+)/CREG cells and can interact with insulin-like growth factor II/mannose 6-phosphate receptor (M6P/IGF2R). Furthermore, that using okadaic acid (an inhibitor of the protein phosphatases 2A) to decrease the number of M6P/IGF2R at the cell surface can significantly inhibit the expression of total RhoA, nuclear SRF and SM a-actin induced by CREG-overexpression. Taken together, as a secreted protein, CREG can enhance the expression of SM alpha-actin by interaction with M6P/IGF2R and perform a pivotal role in the process of VSMCs differentiation and phenotype modulation.
关键词	CELLS RECEPTOR repressor protein E1A phenotype cell vascular smooth muscle human
收录类别	CSCD
语种	英语
CSCD记录号	CSCD:2543256
引用统计	被引频次：7[CSCD] [CSCD记录]
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/151451
专题	中国科学院金属研究所
作者单位	1.中国科学院金属研究所 2.中国科学院大连化学物理研究所
推荐引用方式 GB/T 7714	Han YL,Xu HM,Yan CH,et al. The cellular repressor of E1A-stimulated genes promotes human VSMCs differentiation in vitro mediated by RhoA and SRF[J]. PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,2006,33(5):438-445.
APA	Han YL,Xu HM,Yan CH,Hu Y,Kang J,&Liu HW.(2006).The cellular repressor of E1A-stimulated genes promotes human VSMCs differentiation in vitro mediated by RhoA and SRF.PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,33(5),438-445.
MLA	Han YL,et al."The cellular repressor of E1A-stimulated genes promotes human VSMCs differentiation in vitro mediated by RhoA and SRF".PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS 33.5(2006):438-445.