| Reversible histone acetylation involved in transcriptional regulation of WT1 gene | |
| Alternative Title | Reversible Histone Acetylation Involved in Transcriptional Regulation of WT1 Gene |
| Shao Yangguang1; Lu Jun1; Cheng Cao1; Cui Liguo1; Zhang Guoping1; Huang Baiqu1 | |
| 2007 | |
| Source Publication | ACTA BIOCHIMICA ET BIOPHYSICA SINICA
 |
| ISSN | 1672-9145 |
| Volume | 39Issue:12Pages:931-938 |
| Abstract | To validate the involvement of reversible histone acetylation in the transcriptional regulation of human Wilms' tumor 1 gene (WT1), we analyzed the roles of histone deacetylases (HDACs) and histone acetyltransferase in this epigenetic process. Of the six HDACs (HDAC1-6) examined, HDAC4 and HDAC5 were found to have significant repressing effects on the activity of the WT1 reporter gene, as revealed by luciferase reporter assays and quantitative real-time reverse transcription-polymerase chain reaction assays. Luciferase reporter assays showed that the histone acetyltransferase p300 was able to counteract the HDAC4/HDAC5-mediated repression and that p300/CBP synergized with transcription factors Sp1, c-Myb, and Ets-1 in activation of the WT1 reporter. Chromatin immunoprecipitation experiments showed that p300 promotes the acetylation level of histone H3 at the WT1 intronic enhancer. Based on these data, we proposed a hypothetical model for the involvement of reversible histone acetylation in transcriptional regulation of the WT1 gene. This study provides further insight into the mechanisms of transcriptional regulation of the WT1 gene and WT1-associated diseases treatment. |
| Other Abstract | To validate the involvement of reversible histone acetylation in the transcriptional regulation of human Wilms' tumor 1 gene (WT1), we analyzed the roles of histone deacetylases (HDACs) and histone acetyltransferase in this epigenetic process. Of the six HDACs (HDAC1-6) examined, HDAC4 and HDAC5 were found to have significant repressing effects on the activity of the WT1 reporter gene, as revealed by luciferase reporter assays and quantitative real-time reverse transcription-polymerase chain reaction assays. Luciferase reporter assays showed that the histone acetyltransferase p300 was able to counteract the HDAC4/HDAC5-mediated repression and that p300/CBP synergized with transcription factors Sp1, c-Myb, and Ets-1 in activation of the WT1 reporter. Chromatin immunoprecipitation experiments showed that p300 promotes the acetylation level of histone H3 at the WT1 intronic enhancer. Based on these data, we proposed a hypothetical model for the involvement of reversible histone acetylation in transcriptional regulation of the WT1 gene. This study provides further insight into the mechanisms of transcriptional regulation of the WT1 gene and WT1-associated diseases treatment. |
| Keyword | WILMS-TUMOR ACETYLTRANSFERASE P300 DEACETYLASE ENHANCER PROTEIN GATA-1 SP1 histone acetylation WT1 HDAC p300/CBP transcriptional regulation |
| Indexed By | CSCD |
| Language | 英语 |
| CSCD ID | CSCD:3088023 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.imr.ac.cn/handle/321006/151458 |
| Collection | 中国科学院金属研究所 |
| Affiliation | 1.上海市地震局 2.中国科学院金属研究所 |
| Recommended Citation GB/T 7714 | Shao Yangguang,Lu Jun,Cheng Cao,et al. Reversible histone acetylation involved in transcriptional regulation of WT1 gene[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2007,39(12):931-938. |
| APA | Shao Yangguang,Lu Jun,Cheng Cao,Cui Liguo,Zhang Guoping,&Huang Baiqu.(2007).Reversible histone acetylation involved in transcriptional regulation of WT1 gene.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,39(12),931-938. |
| MLA | Shao Yangguang,et al."Reversible histone acetylation involved in transcriptional regulation of WT1 gene".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 39.12(2007):931-938. |
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